Gene description for ATG5
Gene name autophagy related 5
Gene symbol ATG5
Other names/aliases APG5
APG5-LIKE
APG5L
ASP
hAPG5
Species Homo sapiens
 Database cross references - ATG5
ExoCarta ExoCarta_9474
Vesiclepedia VP_9474
Entrez Gene 9474
HGNC 589
MIM 604261
UniProt Q9H1Y0  
 ATG5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ATG5
Molecular Function
    Atg8 ligase activity GO:0019776 IBA
    protein binding GO:0005515 IPI
Biological Process
    vasodilation GO:0042311 IEA
    autophagosome assembly GO:0000045 ISS
    positive regulation of mucus secretion GO:0070257 IEA
    C-terminal protein lipidation GO:0006501 IBA
    response to drug GO:0042493 IEA
    autophagy GO:0006914 ISS
    blood vessel remodeling GO:0001974 IEA
    regulation of release of sequestered calcium ion into cytosol GO:0051279 IEA
    regulation of cilium assembly GO:1902017 ISS
    negative regulation of protein ubiquitination GO:0031397 IEA
    negative regulation of type I interferon production GO:0032480 TAS
    regulation of cytokine secretion involved in immune response GO:0002739 IEA
    mitochondrion degradation GO:0000422 IBA
    post-translational protein modification GO:0043687 ISS
    heart contraction GO:0060047 IEA
    ventricular cardiac muscle cell development GO:0055015 IEA
    response to fungus GO:0009620 IEA
    nucleophagy GO:0044804 IBA
    cellular response to nitrogen starvation GO:0006995 IBA
    negative regulation of apoptotic process GO:0043066 IEA
    otolith development GO:0048840 IEA
    negative stranded viral RNA replication GO:0039689 IEA
    regulation of reactive oxygen species metabolic process GO:2000377 IEA
    innate immune response GO:0045087 TAS
    apoptotic process GO:0006915 IEA
    negative regulation of histone H4-K16 acetylation GO:2000619 IEA
Subcellular Localization
    autophagosome GO:0005776 IDA
    axoneme GO:0005930 ISS
    membrane GO:0016020 IDA
    Atg12-Atg5-Atg16 complex GO:0034274 IBA
    pre-autophagosomal structure membrane GO:0034045 ISS
    ER-mitochondrion membrane contact site GO:0044233 IEA
    cytoplasm GO:0005737 ISS
 Experiment description of studies that identified ATG5 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ATG5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APG12L  
Affinity Capture-MS Homo sapiens
2 IMPDH2 3615
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ATG5 is involved
PathwayEvidenceSource
Negative regulators of RIG-I/MDA5 signaling TAS Reactome





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