Gene description for ATG5
Gene name autophagy related 5
Gene symbol ATG5
Other names/aliases APG5
APG5-LIKE
APG5L
ASP
hAPG5
Species Homo sapiens
 Database cross references - ATG5
ExoCarta ExoCarta_9474
Vesiclepedia VP_9474
Entrez Gene 9474
HGNC 589
MIM 604261
UniProt Q9H1Y0  
 ATG5 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for ATG5
Molecular Function
    protein binding GO:0005515 IPI
    Atg8-family ligase activity GO:0019776 IDA
Biological Process
    autophagosome assembly GO:0000045 IBA
    autophagosome assembly GO:0000045 IDA
    autophagosome assembly GO:0000045 ISO
    autophagosome assembly GO:0000045 ISS
    mitophagy GO:0000423 IBA
    blood vessel remodeling GO:0001974 IEA
    regulation of cytokine production involved in immune response GO:0002718 IEA
    chromatin organization GO:0006325 IEA
    autophagy GO:0006914 IGI
    autophagy GO:0006914 IMP
    autophagy GO:0006914 ISS
    cellular response to nitrogen starvation GO:0006995 IBA
    response to xenobiotic stimulus GO:0009410 IEA
    response to fungus GO:0009620 IEA
    response to iron(II) ion GO:0010040 IEA
    cardiac muscle cell apoptotic process GO:0010659 IEA
    negative regulation of cardiac muscle cell apoptotic process GO:0010667 IEA
    macroautophagy GO:0016236 IMP
    macroautophagy GO:0016236 ISO
    protein ubiquitination GO:0016567 IEA
    antigen processing and presentation of endogenous antigen GO:0019883 IEA
    negative regulation of protein ubiquitination GO:0031397 IEA
    negative regulation of type I interferon production GO:0032480 ISO
    piecemeal microautophagy of the nucleus GO:0034727 IBA
    aggrephagy GO:0035973 IBA
    aggrephagy GO:0035973 IMP
    negative stranded viral RNA replication GO:0039689 IEA
    vasodilation GO:0042311 IEA
    post-translational protein modification GO:0043687 ISS
    negative thymic T cell selection GO:0045060 IEA
    innate immune response GO:0045087 IEA
    negative regulation of innate immune response GO:0045824 ISO
    otolith development GO:0048840 IEA
    negative regulation of defense response to virus GO:0050687 IDA
    negative regulation of phagocytosis GO:0050765 IEA
    regulation of release of sequestered calcium ion into cytosol GO:0051279 IEA
    defense response to virus GO:0051607 IEA
    ventricular cardiac muscle cell development GO:0055015 IEA
    heart contraction GO:0060047 IEA
    chaperone-mediated autophagy GO:0061684 IEA
    positive regulation of stress granule assembly GO:0062029 IEA
    mucus secretion GO:0070254 IEA
    positive regulation of mucus secretion GO:0070257 IEA
    cellular response to nitrosative stress GO:0071500 IEA
    axonal transport GO:0098930 IEA
    regulation of postsynaptic membrane neurotransmitter receptor levels GO:0099072 IEA
    postsynaptic modulation of chemical synaptic transmission GO:0099170 IEA
    regulation of autophagosome maturation GO:1901096 IMP
    regulation of cilium assembly GO:1902017 ISS
    response to fluoride GO:1902617 IEA
    negative regulation of autophagic cell death GO:1904093 IMP
    positive regulation of viral translation GO:1904973 IDA
    negative regulation of reactive oxygen species metabolic process GO:2000378 IEA
Subcellular Localization
    cytoplasm GO:0005737 ISS
    autophagosome GO:0005776 IBA
    autophagosome GO:0005776 IDA
    cytosol GO:0005829 TAS
    axoneme GO:0005930 ISS
    membrane GO:0016020 IDA
    axon GO:0030424 IEA
    phagocytic vesicle membrane GO:0030670 TAS
    protein-containing complex GO:0032991 NAS
    phagophore assembly site membrane GO:0034045 IBA
    phagophore assembly site membrane GO:0034045 ISO
    phagophore assembly site membrane GO:0034045 ISS
    Atg12-Atg5-Atg16 complex GO:0034274 IBA
    Atg12-Atg5-Atg16 complex GO:0034274 IPI
    mitochondria-associated endoplasmic reticulum membrane contact site GO:0044233 IEA
    phagophore GO:0061908 IBA
    Schaffer collateral - CA1 synapse GO:0098685 IEA
    postsynapse GO:0098794 IEA
    glutamatergic synapse GO:0098978 IEA
    transferase complex GO:1990234 ISO
 Experiment description of studies that identified ATG5 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for ATG5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAP1LC3C  
Affinity Capture-MS Homo sapiens
2 KRT37 8688
Affinity Capture-MS Homo sapiens
3 ISG15 9636
Proximity Label-MS Homo sapiens
4 UTRN 7402
Proximity Label-MS Homo sapiens
5 MDC1  
Affinity Capture-MS Homo sapiens
6 USP13  
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 SYCE1  
Affinity Capture-MS Homo sapiens
8 LDHA 3939
Proximity Label-MS Homo sapiens
9 WIPI2 26100
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 VPS13C 54832
Proximity Label-MS Homo sapiens
11 PSMD10 5716
Affinity Capture-Western Homo sapiens
12 FGFR3 2261
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
13 LUZP1 7798
Proximity Label-MS Homo sapiens
14 ATG13 9776
Affinity Capture-Western Homo sapiens
15 MAP1LC3B 81631
Affinity Capture-MS Homo sapiens
16 IGF2BP3 10643
Affinity Capture-RNA Homo sapiens
17 PGK1 5230
Proximity Label-MS Homo sapiens
18 TECPR1 25851
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Co-purification Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-crystal Structure Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
19 CSRP1 1465
Proximity Label-MS Homo sapiens
20 IMPDH2 3615
Affinity Capture-MS Homo sapiens
21 SRRM2 23524
Proximity Label-MS Homo sapiens
22 ETFA 2108
Proximity Label-MS Homo sapiens
23 CALCOCO2  
Reconstituted Complex Homo sapiens
24 STAT3 6774
Proximity Label-MS Homo sapiens
25 FLG 2312
Proximity Label-MS Homo sapiens
26 CENPQ  
Affinity Capture-MS Homo sapiens
27 ATG16L1 55054
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Co-crystal Structure Homo sapiens
Co-fractionation Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
PCA Homo sapiens
Affinity Capture-MS Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
28 MAVS 57506
Affinity Capture-Western Homo sapiens
29 ATG2B 55102
Proximity Label-MS Homo sapiens
30 FAS 355
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
31 SAMHD1 25939
Proximity Label-MS Homo sapiens
32 CCT2 10576
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 PFN1 5216
Proximity Label-MS Homo sapiens
34 NF2 4771
Affinity Capture-MS Homo sapiens
35 HIST1H4A 8359
Proximity Label-MS Homo sapiens
36 IKBIP 121457
Affinity Capture-MS Homo sapiens
37 RB1CC1 9821
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
38 IFIT5 24138
Proximity Label-MS Homo sapiens
39 TAB3 257397
Co-localization Homo sapiens
40 OPTN 10133
Reconstituted Complex Homo sapiens
41 EXOC4 60412
Affinity Capture-Western Homo sapiens
42 HTT 3064
Affinity Capture-Western Homo sapiens
43 RNF213 57674
Proximity Label-MS Homo sapiens
44 VCP 7415
Two-hybrid Homo sapiens
45 OGT 8473
Reconstituted Complex Homo sapiens
46 TAB2  
Co-localization Homo sapiens
47 MEOX2  
Two-hybrid Homo sapiens
48 ULK1  
Affinity Capture-Western Homo sapiens
49 DHX9 1660
Proximity Label-MS Homo sapiens
50 ATG16L2  
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
51 CYLD  
Affinity Capture-Western Homo sapiens
52 CCDC12  
Affinity Capture-MS Homo sapiens
53 WDFY3 23001
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
54 XPO1 7514
Affinity Capture-MS Homo sapiens
55 TEKT4  
Two-hybrid Homo sapiens
56 ATG12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
57 TJP1 7082
Proximity Label-MS Homo sapiens
58 HSD17B10 3028
Proximity Label-MS Homo sapiens
59 METTL21B  
Affinity Capture-MS Homo sapiens
60 PDPK1 5170
Affinity Capture-Western Homo sapiens
61 KAZN  
Affinity Capture-MS Homo sapiens
62 KRT27 342574
Affinity Capture-MS Homo sapiens
63 STAT1 6772
Proximity Label-MS Homo sapiens
64 AKAP2 11217
Proximity Label-MS Homo sapiens
65 ZC3HAV1 56829
Proximity Label-MS Homo sapiens
66 HLA-A 3105
Proximity Label-MS Homo sapiens
67 LMNA 4000
Proximity Label-MS Homo sapiens
68 TAP1 6890
Proximity Label-MS Homo sapiens
69 BIRC5  
Co-localization Homo sapiens
70 MTHFD1L 25902
Proximity Label-MS Homo sapiens
71 RAB33A 9363
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
72 DHX15 1665
Proximity Label-MS Homo sapiens
73 FADD 8772
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
74 BSN  
Affinity Capture-Western Homo sapiens
75 GBP1 2633
Proximity Label-MS Homo sapiens
76 RPL6 6128
Proximity Label-MS Homo sapiens
77 TTC28 23331
Proximity Label-MS Homo sapiens
78 CCHCR1  
Two-hybrid Homo sapiens
79 CAPN1 823
Biochemical Activity Homo sapiens
80 KEAP1 9817
Two-hybrid Homo sapiens
81 RPS4X 6191
Proximity Label-MS Homo sapiens
82 SQSTM1 8878
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Proximity Label-MS Homo sapiens
83 ATG101  
Affinity Capture-Western Homo sapiens
84 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
85 TKT 7086
Affinity Capture-MS Homo sapiens
86 BCL10  
Affinity Capture-Western Homo sapiens
87 S100A9 6280
Proximity Label-MS Homo sapiens
88 SIRT1  
Affinity Capture-Western Homo sapiens
89 CCT7 10574
Affinity Capture-MS Homo sapiens
90 BCL2L1 598
Affinity Capture-Western Homo sapiens
91 KRT38 8687
Affinity Capture-MS Homo sapiens
92 KXD1 79036
Affinity Capture-MS Homo sapiens
93 RAB33B 83452
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
94 RPL23A 6147
Proximity Label-MS Homo sapiens
95 GNB2L1 10399
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
96 WARS 7453
Proximity Label-MS Homo sapiens
97 CAV1 857
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
98 ATG3 64422
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
99 ALDOA 226
Proximity Label-MS Homo sapiens
100 CAPN2 824
Biochemical Activity Homo sapiens
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