Gene description for PDPK1
Gene name 3-phosphoinositide dependent protein kinase 1
Gene symbol PDPK1
Other names/aliases PDK1
PDPK2
PDPK2P
PRO0461
Species Homo sapiens
 Database cross references - PDPK1
ExoCarta ExoCarta_5170
Vesiclepedia VP_5170
Entrez Gene 5170
HGNC 8816
MIM 605213
UniProt O15530  
 PDPK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PDPK1
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IDA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    phospholipase activator activity GO:0016004 IMP
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    phospholipase binding GO:0043274 IPI
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    type B pancreatic cell development GO:0003323 IEA
    chromatin remodeling GO:0006338 IEA
    protein phosphorylation GO:0006468 IDA
    negative regulation of protein kinase activity GO:0006469 IDA
    hyperosmotic response GO:0006972 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 IMP
    insulin receptor signaling pathway GO:0008286 IEA
    positive regulation of phospholipase activity GO:0010518 IMP
    negative regulation of cardiac muscle cell apoptotic process GO:0010667 IEA
    cell migration GO:0016477 IMP
    peptidyl-threonine phosphorylation GO:0018107 IDA
    calcium-mediated signaling GO:0019722 IMP
    actin cytoskeleton organization GO:0030036 TAS
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IDA
    T cell costimulation GO:0031295 TAS
    activation of protein kinase B activity GO:0032148 IDA
    cellular response to insulin stimulus GO:0032869 IMP
    negative regulation of toll-like receptor signaling pathway GO:0034122 IEA
    intracellular signal transduction GO:0035556 IBA
    intracellular signal transduction GO:0035556 IDA
    regulation of canonical NF-kappaB signal transduction GO:0043122 IMP
    regulation of mast cell degranulation GO:0043304 IEA
    positive regulation of blood vessel endothelial cell migration GO:0043536 IMP
    positive regulation of angiogenesis GO:0045766 IMP
    protein autophosphorylation GO:0046777 TAS
    insulin-like growth factor receptor signaling pathway GO:0048009 IEA
    positive regulation of release of sequestered calcium ion into cytosol GO:0051281 IDA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 TAS
    cellular response to epidermal growth factor stimulus GO:0071364 IMP
    extrinsic apoptotic signaling pathway GO:0097191 IMP
    positive regulation of protein localization to plasma membrane GO:1903078 IMP
    positive regulation of sprouting angiogenesis GO:1903672 IMP
    positive regulation of vascular endothelial cell proliferation GO:1905564 IMP
    negative regulation of endothelial cell apoptotic process GO:2000352 IMP
Subcellular Localization
    nucleus GO:0005634 IEA
    cytoplasm GO:0005737 IDA
    cytoplasm GO:0005737 IEA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    focal adhesion GO:0005925 IEA
    postsynaptic density GO:0014069 IEA
    membrane GO:0016020 IEA
    cytoplasmic vesicle GO:0031410 IEA
    cell projection GO:0042995 IDA
 Experiment description of studies that identified PDPK1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PDPK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SENP3 26168
Affinity Capture-Western Homo sapiens
2 PRKCD 5580
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
3 ATG5 9474
Affinity Capture-Western Homo sapiens
4 FAXC  
Affinity Capture-MS Homo sapiens
5 DDIT4  
Two-hybrid Homo sapiens
6 APP 351
Reconstituted Complex Homo sapiens
7 Cct2 12461
Affinity Capture-MS Mus musculus
8 RPS6KA3 6197
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
9 CASP3 836
Affinity Capture-MS Homo sapiens
10 NR2F2  
Co-fractionation Homo sapiens
11 SLC31A1 1317
Affinity Capture-MS Homo sapiens
12 PIK3C3 5289
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
13 XPO7 23039
Two-hybrid Homo sapiens
14 PEA15 8682
Two-hybrid Homo sapiens
15 Kctd5  
Affinity Capture-MS Mus musculus
16 C2orf44  
Two-hybrid Homo sapiens
17 CPA4 51200
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 YWHAH 7533
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
19 SOCS3 9021
Two-hybrid Homo sapiens
20 PKN2 5586
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
21 WDR5 11091
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Protein-peptide Homo sapiens
22 SMARCB1 6598
Two-hybrid Homo sapiens
23 PLS3 5358
Co-fractionation Homo sapiens
24 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 ZDHHC17 23390
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
26 ZNF133  
Two-hybrid Homo sapiens
27 TBL2 26608
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 PHAX  
Two-hybrid Homo sapiens
29 PSME1 5720
Co-fractionation Homo sapiens
30 PNO1 56902
Two-hybrid Homo sapiens
31 RBKS  
Co-fractionation Homo sapiens
32 UBE2I 7329
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
33 RPS6KB1 6198
Affinity Capture-Western Homo sapiens
34 UBFD1 56061
Co-fractionation Homo sapiens
35 GIT1 28964
Two-hybrid Homo sapiens
36 WIPI2 26100
Affinity Capture-Western Homo sapiens
37 POLDIP2 26073
Two-hybrid Homo sapiens
38 LUC7L2 51631
Two-hybrid Homo sapiens
39 PKN1 5585
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
40 YWHAQ 10971
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
41 MAPK8 5599
Reconstituted Complex Homo sapiens
42 SND1 27044
Affinity Capture-MS Homo sapiens
43 SRPK1 6732
Biochemical Activity Homo sapiens
44 TCAP  
Two-hybrid Homo sapiens
45 KATNBL1  
Two-hybrid Homo sapiens
46 XPO1 7514
Affinity Capture-MS Homo sapiens
47 BAG2 9532
Affinity Capture-MS Homo sapiens
48 SEC13 6396
Co-fractionation Homo sapiens
49 PRKCZ 5590
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
50 SLC9A3R2 9351
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
51 CARD11  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
52 EGFR 1956
Negative Genetic Homo sapiens
53 ZC3HC1  
Two-hybrid Homo sapiens
54 SBF1 6305
Two-hybrid Homo sapiens
55 MAP3K7  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
56 LRRC16A 55604
Co-fractionation Homo sapiens
57 SDC2 6383
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
58 STRAP 11171
Affinity Capture-Western Homo sapiens
59 PRKCI 5584
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
60 PRKCQ 5588
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
61 NRBP1 29959
Affinity Capture-MS Homo sapiens
62 RPA3 6119
Proximity Label-MS Homo sapiens
63 SEC31A 22872
Co-fractionation Homo sapiens
64 BLMH 642
Two-hybrid Homo sapiens
65 AKT2 208
Affinity Capture-Western Homo sapiens
66 PDPK1 5170
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-MS Homo sapiens
67 ILK 3611
Affinity Capture-Western Homo sapiens
68 SCRN2 90507
Affinity Capture-MS Homo sapiens
69 COX2 4513
Affinity Capture-Western Homo sapiens
70 AKT1 207
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
71 RBBP5 5929
Affinity Capture-MS Homo sapiens
72 PA2G4 5036
Affinity Capture-MS Homo sapiens
73 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
74 SGK1  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
75 FAM199X 139231
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
76 CDSN 1041
Affinity Capture-MS Homo sapiens
77 G3BP1 10146
Affinity Capture-MS Homo sapiens
78 MRI1 84245
Co-fractionation Homo sapiens
79 PRKACA 5566
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
80 FAM213B 127281
Two-hybrid Homo sapiens
81 IDE 3416
Co-fractionation Homo sapiens
82 MAP1LC3A 84557
Affinity Capture-Western Homo sapiens
83 PCCB 5096
Co-fractionation Homo sapiens
84 XRCC6 2547
Two-hybrid Homo sapiens
85 KRAS 3845
Negative Genetic Homo sapiens
86 PRKCB 5579
Reconstituted Complex Homo sapiens
87 SERPINA4 5267
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 PRSS23 11098
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which PDPK1 is involved
PathwayEvidenceSource
Activation of AKT2 TAS Reactome
Activation of NMDA receptors and postsynaptic events IEA Reactome
Adaptive Immune System TAS Reactome
C-type lectin receptors (CLRs) TAS Reactome
CD28 co-stimulation TAS Reactome
CD28 dependent PI3K/Akt signaling TAS Reactome
Cellular responses to mechanical stimuli TAS Reactome
Cellular responses to stimuli TAS Reactome
CLEC7A (Dectin-1) signaling TAS Reactome
Constitutive Signaling by AKT1 E17K in Cancer TAS Reactome
Costimulation by the CD28 family TAS Reactome
CREB1 phosphorylation through NMDA receptor-mediated activation of RAS signaling IEA Reactome
Disease TAS Reactome
Diseases of signal transduction by growth factor receptors and second messengers TAS Reactome
Downstream TCR signaling TAS Reactome
ESR-mediated signaling TAS Reactome
ESR-mediated signaling IEA Reactome
Estrogen-stimulated signaling through PRKCZ TAS Reactome
Estrogen-stimulated signaling through PRKCZ IEA Reactome
Extra-nuclear estrogen signaling TAS Reactome
Extra-nuclear estrogen signaling IEA Reactome
Fc epsilon receptor (FCERI) signaling TAS Reactome
FCERI mediated NF-kB activation TAS Reactome
G beta:gamma signalling through PI3Kgamma TAS Reactome
G-protein beta:gamma signalling TAS Reactome
Gene expression (Transcription) TAS Reactome
Generic Transcription Pathway TAS Reactome
GPCR downstream signalling TAS Reactome
GPVI-mediated activation cascade TAS Reactome
GPVI-mediated activation cascade IEA Reactome
Hemostasis TAS Reactome
Hemostasis IEA Reactome
High laminar flow shear stress activates signaling by PIEZO1 and PECAM1:CDH5:KDR in endothelial cells TAS Reactome
IGF1R signaling cascade TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Innate Immune System TAS Reactome
Insulin receptor signalling cascade TAS Reactome
Integrin signaling TAS Reactome
Intracellular signaling by second messengers TAS Reactome
IRS-mediated signalling TAS Reactome
IRS-related events triggered by IGF1R TAS Reactome
Neuronal System IEA Reactome
Neurotransmitter receptors and postsynaptic signal transmission IEA Reactome
PI3K Cascade TAS Reactome
PI3K/AKT Signaling in Cancer TAS Reactome
PIP3 activates AKT signaling TAS Reactome
Platelet activation, signaling and aggregation TAS Reactome
Platelet activation, signaling and aggregation IEA Reactome
Platelet Aggregation (Plug Formation) TAS Reactome
Post NMDA receptor activation events IEA Reactome
Regulation of TP53 Activity TAS Reactome
Regulation of TP53 Degradation TAS Reactome
Regulation of TP53 Expression and Degradation TAS Reactome
Response of endothelial cells to shear stress TAS Reactome
RHO GTPase Effectors TAS Reactome
RHO GTPases activate PKNs TAS Reactome
RNA Polymerase II Transcription TAS Reactome
Role of LAT2/NTAL/LAB on calcium mobilization TAS Reactome
RSK activation IEA Reactome
SARS-CoV Infections TAS Reactome
SARS-CoV-1 Infection TAS Reactome
SARS-CoV-1 targets host intracellular signalling and regulatory pathways TAS Reactome
SARS-CoV-1-host interactions TAS Reactome
SARS-CoV-2 Infection TAS Reactome
SARS-CoV-2 targets host intracellular signalling and regulatory pathways TAS Reactome
SARS-CoV-2-host interactions TAS Reactome
Signal Transduction TAS Reactome
Signal Transduction IEA Reactome
Signaling by GPCR TAS Reactome
Signaling by Insulin receptor TAS Reactome
Signaling by Nuclear Receptors TAS Reactome
Signaling by Nuclear Receptors IEA Reactome
Signaling by Receptor Tyrosine Kinases TAS Reactome
Signaling by Rho GTPases TAS Reactome
Signaling by Rho GTPases, Miro GTPases and RHOBTB3 TAS Reactome
Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R) TAS Reactome
Signaling by VEGF TAS Reactome
TCR signaling TAS Reactome
Transcriptional Regulation by TP53 TAS Reactome
Transmission across Chemical Synapses IEA Reactome
VEGFA-VEGFR2 Pathway TAS Reactome
VEGFR2 mediated cell proliferation TAS Reactome
VEGFR2 mediated vascular permeability TAS Reactome
Viral Infection Pathways TAS Reactome





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