Gene description for PDPK1
Gene name 3-phosphoinositide dependent protein kinase 1
Gene symbol PDPK1
Other names/aliases PDK1
PDPK2
PDPK2P
PRO0461
Species Homo sapiens
 Database cross references - PDPK1
ExoCarta ExoCarta_5170
Vesiclepedia VP_5170
Entrez Gene 5170
HGNC 8816
MIM 605213
UniProt O15530  
 PDPK1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for PDPK1
Molecular Function
    ATP binding GO:0005524 IEA
    phospholipase activator activity GO:0016004 IMP
    protein binding GO:0005515 IPI
    protein serine/threonine kinase activity GO:0004674 TAS
    kinase activity GO:0016301 EXP
    insulin receptor binding GO:0005158 IEA
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IDA
    protein kinase binding GO:0019901 IEA
    phospholipase binding GO:0043274 IPI
Biological Process
    actin cytoskeleton organization GO:0030036 TAS
    blood coagulation GO:0007596 TAS
    intracellular signal transduction GO:0035556 IDA
    extrinsic apoptotic signaling pathway GO:0097191 IMP
    insulin receptor signaling pathway GO:0008286 TAS
    regulation of transcription, DNA-templated GO:0006355 IEA
    positive regulation of phospholipase activity GO:0010518 IMP
    negative regulation of cardiac muscle cell apoptotic process GO:0010667 IEA
    calcium-mediated signaling GO:0019722 IMP
    protein autophosphorylation GO:0046777 TAS
    innate immune response GO:0045087 TAS
    activation of protein kinase B activity GO:0032148 IDA
    T cell costimulation GO:0031295 TAS
    small GTPase mediated signal transduction GO:0007264 TAS
    cell migration GO:0016477 IMP
    peptidyl-threonine phosphorylation GO:0018107 IDA
    regulation of endothelial cell migration GO:0010594 IEA
    focal adhesion assembly GO:0048041 IEA
    negative regulation of protein kinase activity GO:0006469 IDA
    positive regulation of establishment of protein localization to plasma membrane GO:0090004 IMP
    transcription, DNA-templated GO:0006351 IEA
    platelet activation GO:0030168 TAS
    hyperosmotic response GO:0006972 IEA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    cellular response to insulin stimulus GO:0032869 IMP
    positive regulation of release of sequestered calcium ion into cytosol GO:0051281 IDA
    phosphatidylinositol-mediated signaling GO:0048015 TAS
    Fc-epsilon receptor signaling pathway GO:0038095 TAS
    negative regulation of toll-like receptor signaling pathway GO:0034122 IEA
    regulation of mast cell degranulation GO:0043304 IEA
    synaptic transmission GO:0007268 TAS
    protein phosphorylation GO:0006468 IEA
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    regulation of I-kappaB kinase/NF-kappaB signaling GO:0043122 IMP
    T cell receptor signaling pathway GO:0050852 TAS
    cellular response to epidermal growth factor stimulus GO:0071364 IMP
    negative regulation of transforming growth factor beta receptor signaling pathway GO:0030512 IDA
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    type B pancreatic cell development GO:0003323 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
Subcellular Localization
    cytoplasmic membrane-bounded vesicle GO:0016023 IEA
    focal adhesion GO:0005925 IEA
    nucleoplasm GO:0005654 TAS
    cytosol GO:0005829 TAS
    membrane GO:0016020 IEA
    plasma membrane GO:0005886 TAS
    cell projection GO:0042995 IDA
    cytoplasm GO:0005737 IEA
 Experiment description of studies that identified PDPK1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for PDPK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAH 7533
Invivo Homo sapiens
Invitro Homo sapiens
2 PKN1 5585
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
3 YWHAQ 10971
Invivo Homo sapiens
Invitro Homo sapiens
4 PRKCI 5584
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
5 FTS  
Affinity Capture-MS Homo sapiens
6 RPS6KA3 6197
Affinity Capture-Western Homo sapiens
7 PKN2 5586
Affinity Capture-Western Homo sapiens
8 PRKCZ 5590
Two-hybrid Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
9 PRKCB 5579
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
10 SLC9A3R2 9351
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
11 SGK1  
Reconstituted Complex Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
12 PRSS23 11098
Two-hybrid Homo sapiens
13 PRKCD 5580
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
14 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
15 AKT1 207
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
16 PDPK1 5170
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
17 PRKACA 5566
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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