Gene description for PKN2
Gene name protein kinase N2
Gene symbol PKN2
Other names/aliases PAK2
PRK2
PRKCL2
PRO2042
Pak-2
STK7
Species Homo sapiens
 Database cross references - PKN2
ExoCarta ExoCarta_5586
Entrez Gene 5586
HGNC 9406
MIM 602549
UniProt Q16513  
 PKN2 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Malignant pleural effusions 14975938    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for PKN2
Molecular Function
    RNA polymerase binding GO:0070063 IPI
    protein kinase activity GO:0004672 TAS
    protein kinase C activity GO:0004697 IEA
    kinase activity GO:0016301 IDA
    poly(A) RNA binding GO:0044822 IDA
    ATP binding GO:0005524 IEA
    histone deacetylase binding GO:0042826 IDA
    protein serine/threonine kinase activity GO:0004674 IDA
    protein binding GO:0005515 IPI
Biological Process
    cell adhesion GO:0007155 IEA
    positive regulation of cytokinesis GO:0032467 IMP
    transcription, DNA-templated GO:0006351 IEA
    regulation of cell motility GO:2000145 IMP
    apoptotic process GO:0006915 IEA
    positive regulation of mitotic cell cycle GO:0045931 IMP
    signal transduction GO:0007165 TAS
    positive regulation of viral genome replication GO:0045070 IMP
    epithelial cell migration GO:0010631 IDA
    regulation of transcription, DNA-templated GO:0006355 IEA
    small GTPase mediated signal transduction GO:0007264 TAS
    cell division GO:0051301 IEA
    apical junction assembly GO:0043297 IMP
    cell cycle GO:0007049 IEA
    protein phosphorylation GO:0006468 IDA
Subcellular Localization
    apical junction complex GO:0043296 IDA
    nucleus GO:0005634 IDA
    plasma membrane GO:0005886 IDA
    cytoplasm GO:0005737 IDA
    cleavage furrow GO:0032154 IDA
    nucleoplasm GO:0005654 IDA
    midbody GO:0030496 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    cell junction GO:0030054 IDA
    centrosome GO:0005813 IDA
    lamellipodium GO:0030027 IDA
    cytosol GO:0005829 TAS
    intermediate filament cytoskeleton GO:0045111 IDA
 Experiment description of studies that identified PKN2 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 52
ISEV standards
✔
EM
EV Biophysical techniques
✔
HSP90
EV Cytosolic markers
✔
MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 14975938    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes isolated from human malignant pleural effusions.
Authors Bard MP, Hegmans JP, Hemmes A, Luider TM, Willemsen R, Severijnen LA, van Meerbeeck JP, Burgers SA, Hoogsteden HC, Lambrecht BN
Journal name AJRCMB
Publication year 2004
Sample Malignant pleural effusions
Sample name Pleural Fluid - Lung cancer
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
5
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for PKN2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 NCK2 8440
Invitro Homo sapiens
2 PDK1  
Affinity Capture-Western Homo sapiens
3 MAP3K2  
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Two-hybrid Homo sapiens
4 AKT1 207
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 NCK1 4690
Reconstituted Complex Homo sapiens
6 RHOA 387
Reconstituted Complex Homo sapiens
7 PLCG1 5335
Reconstituted Complex Homo sapiens
8 PTPN13 5783
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
9 PDPK1  
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which PKN2 is involved
PathwayEvidenceSource
RHO GTPases activate PKNs TAS Reactome





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