Gene description for MAP3K2
Gene name mitogen-activated protein kinase kinase kinase 2
Gene symbol MAP3K2
Other names/aliases MEKK2
MEKK2B
Species Homo sapiens
 Database cross references - MAP3K2
ExoCarta ExoCarta_10746
Vesiclepedia VP_10746
Entrez Gene 10746
HGNC 6854
MIM 609487
UniProt Q9Y2U5  
 MAP3K2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MAP3K2
Molecular Function
    protein kinase activity GO:0004672 IDA
    protein serine/threonine kinase activity GO:0004674 IBA
    JUN kinase kinase kinase activity GO:0004706 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    protein kinase binding GO:0019901 IPI
    metal ion binding GO:0046872 IEA
    protein serine kinase activity GO:0106310 IEA
Biological Process
    MAPK cascade GO:0000165 IEA
    intracellular signal transduction GO:0035556 IDA
    cellular response to mechanical stimulus GO:0071260 IEP
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 IDA
 Experiment description of studies that identified MAP3K2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAP3K2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 STUB1 10273
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
2 YWHAH 7533
Affinity Capture-MS Homo sapiens
3 SFN 2810
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 TRAF7  
Affinity Capture-MS Homo sapiens
5 BIRC2  
Biochemical Activity Homo sapiens
6 USP2  
Biochemical Activity Homo sapiens
7 BTRC 8945
Affinity Capture-Western Homo sapiens
8 MAP2K7 5609
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
Biochemical Activity Homo sapiens
9 XIAP  
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
10 NEDD4 4734
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
11 GSK3B 2932
Affinity Capture-Western Homo sapiens
12 MAP2K4 6416
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
13 YWHAZ 7534
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 YWHAB 7529
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 Map2k4  
Biochemical Activity Mus musculus
16 STK38 11329
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
17 YWHAE 7531
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 CTNNB1 1499
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
19 MAP3K3 4215
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
20 Stub1 56424
Two-hybrid Mus musculus
21 IKBKG 8517
Reconstituted Complex Homo sapiens
22 SMURF1 57154
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
23 MAP3K2 10746
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
24 MAP2K5  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
25 PIBF1  
Two-hybrid Homo sapiens
26 PARK2  
Affinity Capture-MS Homo sapiens
27 CBY1  
Affinity Capture-MS Homo sapiens
28 HSP90AA1 3320
Affinity Capture-Luminescence Homo sapiens
29 STK38L 23012
Affinity Capture-Western Homo sapiens
30 AXIN2 8313
Affinity Capture-Western Homo sapiens
31 AXIN1  
Affinity Capture-Western Homo sapiens
32 LATS1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
33 MAP2K3 5606
Biochemical Activity Homo sapiens
34 MAPK7  
Affinity Capture-Western Homo sapiens
35 YAP1 10413
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
36 STX3 6809
Affinity Capture-MS Homo sapiens
37 TRIM21 6737
Affinity Capture-MS Homo sapiens
38 WWTR1 25937
Affinity Capture-Western Homo sapiens
39 RAB3IP  
Affinity Capture-MS Homo sapiens
40 YWHAQ 10971
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 STAMBP 10617
Biochemical Activity Homo sapiens
43 MAPK8 5599
Affinity Capture-Western Homo sapiens
Co-fractionation Homo sapiens
44 LATS2 26524
Affinity Capture-Western Homo sapiens
45 SH2D2A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
46 NEDD4L 23327
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which MAP3K2 is involved
No pathways found





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