Gene description for MAP3K2
Gene name mitogen-activated protein kinase kinase kinase 2
Gene symbol MAP3K2
Other names/aliases MEKK2
MEKK2B
Species Homo sapiens
 Database cross references - MAP3K2
ExoCarta ExoCarta_10746
Vesiclepedia VP_10746
Entrez Gene 10746
HGNC 6854
MIM 609487
UniProt Q9Y2U5  
 MAP3K2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for MAP3K2
Molecular Function
    protein kinase activity GO:0004672 IDA
    MAP kinase kinase kinase activity GO:0004709 IEA
    ATP binding GO:0005524 IEA
    MAP kinase kinase activity GO:0004708 IBA
    protein kinase binding GO:0019901 IPI
    metal ion binding GO:0046872 IEA
    protein binding GO:0005515 IPI
    protein serine/threonine kinase activity GO:0004674 TAS
Biological Process
    positive regulation of transcription, DNA-templated GO:0045893 IDA
    cellular response to mechanical stimulus GO:0071260 IEP
    activation of JUN kinase activity GO:0007257 TAS
    activation of MAPK activity GO:0000187 TAS
    protein phosphorylation GO:0006468 TAS
    activation of MAPKK activity GO:0000186 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IEA
    intracellular GO:0005622 IBA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified MAP3K2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for MAP3K2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAP2K7 5609
Invivo Homo sapiens
Invitro Homo sapiens
2 YWHAE 7531
Reconstituted Complex Homo sapiens
3 SFN 2810
Affinity Capture-MS Homo sapiens
4 PKN2 5586
Invivo Homo sapiens
Two-hybrid Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 MAPK8 5599
Invivo Homo sapiens
Invitro Homo sapiens
6 MAP2K5  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
7 SH2D2A  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 MAP2K4 6416
Invitro Homo sapiens
View the network image/svg+xml
 Pathways in which MAP3K2 is involved
No pathways found





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