Gene description for MAP2K3
Gene name mitogen-activated protein kinase kinase 3
Gene symbol MAP2K3
Other names/aliases MAPKK3
MEK3
MKK3
PRKMK3
SAPKK-2
SAPKK2
Species Homo sapiens
 Database cross references - MAP2K3
ExoCarta ExoCarta_5606
Vesiclepedia VP_5606
Entrez Gene 5606
HGNC 6843
MIM 602315
UniProt P46734  
 MAP2K3 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for MAP2K3
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IEA
    MAP kinase kinase activity GO:0004708 IBA
    MAP kinase kinase activity GO:0004708 IDA
    MAP kinase kinase activity GO:0004708 TAS
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    JUN kinase kinase activity GO:0008545 IEA
    protein kinase binding GO:0019901 IPI
    histone H3Y41 kinase activity GO:0035401 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2AXY142 kinase activity GO:0140801 IEA
Biological Process
    MAPK cascade GO:0000165 IBA
    regulation of cytokine production GO:0001817 IEA
    response to ischemia GO:0002931 IEA
    chromatin remodeling GO:0006338 IEA
    inflammatory response GO:0006954 IEA
    signal transduction GO:0007165 TAS
    JNK cascade GO:0007254 IEA
    heart development GO:0007507 IEA
    stress-activated protein kinase signaling cascade GO:0031098 IDA
    negative regulation of hippo signaling GO:0035331 IMP
    cellular response to vascular endothelial growth factor stimulus GO:0035924 IMP
    p38MAPK cascade GO:0038066 IMP
    p38MAPK cascade GO:0038066 TAS
    positive regulation of MAPK cascade GO:0043410 IEA
    positive regulation of blood vessel endothelial cell migration GO:0043536 IMP
    positive regulation of protein kinase activity GO:0045860 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    cardiac muscle contraction GO:0060048 IEA
    cellular response to lipopolysaccharide GO:0071222 IEA
    cellular response to sorbitol GO:0072709 IEA
    cellular senescence GO:0090398 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 TAS
    cytosol GO:0005829 TAS
    membrane GO:0016020 HDA
 Experiment description of studies that identified MAP2K3 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAP2K3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 PPP2R4 5524
Affinity Capture-Western Homo sapiens
3 FOXA3  
Affinity Capture-MS Homo sapiens
4 RPS3A 6189
Affinity Capture-MS Homo sapiens
5 AKAP13 11214
Co-fractionation Homo sapiens
6 KLK5 25818
Affinity Capture-MS Homo sapiens
7 RPL30 6156
Affinity Capture-MS Homo sapiens
8 SRPK2 6733
Affinity Capture-MS Homo sapiens
9 TARDBP 23435
Affinity Capture-MS Homo sapiens
10 BUD13  
Affinity Capture-MS Homo sapiens
11 FFAR1  
Affinity Capture-MS Homo sapiens
12 APP 351
Reconstituted Complex Homo sapiens
13 APEX1 328
Affinity Capture-RNA Homo sapiens
14 DIMT1 27292
Affinity Capture-MS Homo sapiens
15 MYH6 4624
Affinity Capture-MS Homo sapiens
16 TGFB1 7040
Affinity Capture-MS Homo sapiens
17 DDX24 57062
Affinity Capture-MS Homo sapiens
18 KLK15  
Affinity Capture-MS Homo sapiens
19 SPAG9 9043
Affinity Capture-Western Homo sapiens
20 ZAK 51776
Co-fractionation Homo sapiens
21 RPL24 6152
Affinity Capture-MS Homo sapiens
22 NPM1 4869
Affinity Capture-MS Homo sapiens
23 DUSP2  
Affinity Capture-MS Homo sapiens
24 MAPK14 1432
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
FRET Homo sapiens
25 TINF2  
Two-hybrid Homo sapiens
26 MAP3K3 4215
Biochemical Activity Homo sapiens
27 RPL29 6159
Affinity Capture-MS Homo sapiens
28 ZNF598 90850
Affinity Capture-MS Homo sapiens
29 RPL19 6143
Affinity Capture-MS Homo sapiens
30 RPS26 6231
Affinity Capture-MS Homo sapiens
31 MAPK8IP2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
32 MAPK8IP3  
Affinity Capture-Western Homo sapiens
33 CD70 970
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 MAPK3 5595
Reconstituted Complex Homo sapiens
35 MAP3K2 10746
Biochemical Activity Homo sapiens
36 ANKRD17 26057
Affinity Capture-MS Homo sapiens
37 RPL4 6124
Affinity Capture-MS Homo sapiens
38 NPHS1 4868
Phenotypic Suppression Homo sapiens
39 GOLGA2 2801
Affinity Capture-MS Homo sapiens
40 MAPK8IP1 9479
Affinity Capture-Western Homo sapiens
41 UBFD1 56061
Cross-Linking-MS (XL-MS) Homo sapiens
42 DDX5 1655
Affinity Capture-MS Homo sapiens
43 U2AF2 11338
Affinity Capture-MS Homo sapiens
44 PKN1 5585
Affinity Capture-Western Homo sapiens
45 DHX9 1660
Affinity Capture-MS Homo sapiens
46 FOXS1  
Affinity Capture-MS Homo sapiens
47 SRPK1 6732
Affinity Capture-MS Homo sapiens
48 UBXN6 80700
Affinity Capture-MS Homo sapiens
49 SLC25A5 292
Affinity Capture-MS Homo sapiens
50 PSG11  
Affinity Capture-MS Homo sapiens
51 TUBB 203068
Affinity Capture-MS Homo sapiens
52 APLNR  
Affinity Capture-MS Homo sapiens
53 MICAL2 9645
Affinity Capture-MS Homo sapiens
54 CUL3 8452
Affinity Capture-MS Homo sapiens
55 YWHAZ 7534
Affinity Capture-MS Homo sapiens
56 RPL21 6144
Affinity Capture-MS Homo sapiens
57 RPL3 6122
Affinity Capture-MS Homo sapiens
58 EGFR 1956
Affinity Capture-MS Homo sapiens
59 FOXL1  
Affinity Capture-MS Homo sapiens
60 METTL21B  
Affinity Capture-MS Homo sapiens
61 HNRNPUL1 11100
Affinity Capture-MS Homo sapiens
62 RPL7 6129
Affinity Capture-MS Homo sapiens
63 HIP1R 9026
Co-fractionation Homo sapiens
64 HNRNPCL2 440563
Affinity Capture-MS Homo sapiens
65 MAP2K3 5606
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
66 PCBP1 5093
Affinity Capture-MS Homo sapiens
67 TAOK2 9344
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
Co-localization Homo sapiens
68 DNAI2  
Affinity Capture-MS Homo sapiens
69 MAP3K4 4216
Biochemical Activity Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
70 CDK20  
Affinity Capture-MS Homo sapiens
71 HSPD1 3329
Affinity Capture-MS Homo sapiens
72 RPL13 6137
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
73 DVL1 1855
Affinity Capture-MS Homo sapiens
74 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
75 MAP2K6 5608
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Phenotypic Enhancement Homo sapiens
Co-localization Homo sapiens
76 ALDOC 230
Two-hybrid Homo sapiens
77 SIRT6  
Affinity Capture-MS Homo sapiens
78 ILF3 3609
Affinity Capture-MS Homo sapiens
79 SMAD7  
Affinity Capture-Western Homo sapiens
80 PLCB2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
81 MFSD4  
Affinity Capture-MS Homo sapiens
82 ASIC4  
Affinity Capture-MS Homo sapiens
83 HIST1H2BC 8347
Cross-Linking-MS (XL-MS) Homo sapiens
84 TUBB3 10381
Affinity Capture-MS Homo sapiens
85 Mapk14  
Affinity Capture-Western Mus musculus
86 HOXB9  
Affinity Capture-MS Homo sapiens
87 HSP90AA1 3320
Affinity Capture-MS Homo sapiens
88 HNRNPK 3190
Affinity Capture-MS Homo sapiens
89 TRIP12 9320
Affinity Capture-MS Homo sapiens
90 RPS28 6234
Affinity Capture-MS Homo sapiens
91 NCL 4691
Affinity Capture-MS Homo sapiens
92 LRRK2 120892
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
93 CDK9 1025
Affinity Capture-MS Homo sapiens
94 RPS6 6194
Affinity Capture-MS Homo sapiens
95 RPL28 6158
Affinity Capture-MS Homo sapiens
96 SNW1 22938
Affinity Capture-MS Homo sapiens
97 MYC  
Dosage Lethality Homo sapiens
98 MATN2 4147
Affinity Capture-MS Homo sapiens
99 CCR1  
Affinity Capture-MS Homo sapiens
100 CUL4A 8451
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which MAP2K3 is involved
PathwayEvidenceSource
activated TAK1 mediates p38 MAPK activation TAS Reactome
Bacterial Infection Pathways TAS Reactome
Cellular responses to stimuli TAS Reactome
Cellular responses to stress TAS Reactome
Cellular Senescence TAS Reactome
Cytokine Signaling in Immune system TAS Reactome
Disease TAS Reactome
Immune System TAS Reactome
Infectious disease TAS Reactome
Innate Immune System TAS Reactome
Interleukin-17 signaling TAS Reactome
MAP kinase activation TAS Reactome
MyD88 cascade initiated on plasma membrane TAS Reactome
MyD88 dependent cascade initiated on endosome TAS Reactome
MyD88-independent TLR4 cascade TAS Reactome
MyD88:MAL(TIRAP) cascade initiated on plasma membrane TAS Reactome
Oxidative Stress Induced Senescence TAS Reactome
Signaling by Interleukins TAS Reactome
Toll Like Receptor 10 (TLR10) Cascade TAS Reactome
Toll Like Receptor 2 (TLR2) Cascade TAS Reactome
Toll Like Receptor 3 (TLR3) Cascade TAS Reactome
Toll Like Receptor 4 (TLR4) Cascade TAS Reactome
Toll Like Receptor 5 (TLR5) Cascade TAS Reactome
Toll Like Receptor 7/8 (TLR7/8) Cascade TAS Reactome
Toll Like Receptor 9 (TLR9) Cascade TAS Reactome
Toll Like Receptor TLR1:TLR2 Cascade TAS Reactome
Toll Like Receptor TLR6:TLR2 Cascade TAS Reactome
Toll-like Receptor Cascades TAS Reactome
TRAF6 mediated induction of NFkB and MAP kinases upon TLR7/8 or 9 activation TAS Reactome
TRIF (TICAM1)-mediated TLR4 signaling TAS Reactome
Uptake and actions of bacterial toxins TAS Reactome
Uptake and function of anthrax toxins TAS Reactome





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