Gene description for MAP2K6
Gene name mitogen-activated protein kinase kinase 6
Gene symbol MAP2K6
Other names/aliases MAPKK6
MEK6
MKK6
PRKMK6
SAPKK-3
SAPKK3
Species Homo sapiens
 Database cross references - MAP2K6
ExoCarta ExoCarta_5608
Vesiclepedia VP_5608
Entrez Gene 5608
HGNC 6846
MIM 601254
UniProt P52564  
 MAP2K6 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells Unpublished / Not applicable
Thymus 23844026    
 Gene ontology annotations for MAP2K6
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    protein kinase binding GO:0019901 IPI
    protein tyrosine kinase activity GO:0004713 IEA
    ATP binding GO:0005524 IEA
    MAP kinase kinase activity GO:0004708 IEA
    protein binding GO:0005515 IPI
Biological Process
    cardiac muscle contraction GO:0060048 IEA
    nucleotide-binding domain, leucine rich repeat containing receptor signaling pathway GO:0035872 TAS
    muscle cell differentiation GO:0042692 TAS
    transcription, DNA-templated GO:0006351 IEA
    apoptotic process GO:0006915 IEA
    ovulation cycle process GO:0022602 IEA
    signal transduction by protein phosphorylation GO:0023014 IBA
    regulation of transcription, DNA-templated GO:0006355 IEA
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    innate immune response GO:0045087 TAS
    regulation of mitotic cell cycle GO:0007346 IBA
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    regulation of apoptotic process GO:0042981 IBA
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    positive regulation of muscle cell differentiation GO:0051149 TAS
    positive regulation of prostaglandin secretion GO:0032308 IEA
    response to ischemia GO:0002931 IEA
    cell cycle arrest GO:0007050 TAS
    response to drug GO:0042493 IEA
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    signal transduction GO:0007165 TAS
    nucleotide-binding oligomerization domain containing signaling pathway GO:0070423 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    activation of MAPK activity GO:0000187 TAS
    positive regulation of apoptotic process GO:0043065 IEA
    stress-activated MAPK cascade GO:0051403 TAS
    DNA damage induced protein phosphorylation GO:0006975 TAS
    cellular response to sorbitol GO:0072709 IEA
    toll-like receptor signaling pathway GO:0002224 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    stress-activated protein kinase signaling cascade GO:0031098 IBA
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    peptidyl-tyrosine phosphorylation GO:0018108 IEA
    positive regulation of nitric-oxide synthase biosynthetic process GO:0051770 IEA
    toll-like receptor 5 signaling pathway GO:0034146 TAS
Subcellular Localization
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IBA
    cytoskeleton GO:0005856 IEA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified MAP2K6 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 235
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
6
Experiment ID 126
MISEV standards
✘ EV Biophysical techniques
✔
GAPDH
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
7
Experiment ID 217
MISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|CD81|CD9|CD63
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MAP2K6
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 MAPK14 1432
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
2 MAP2K6 5608
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
3 MAP3K7  
Biochemical Activity Homo sapiens
Biochemical Activity Homo sapiens
4 TAOK2 9344
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
5 SMAD7  
Affinity Capture-Western Homo sapiens
6 MAP3K5 4217
Reconstituted Complex Homo sapiens
7 PLCB2  
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
8 MAP3K4 4216
Invitro Homo sapiens
Invivo Homo sapiens
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