Gene description for C1QA
Gene name complement component 1, q subcomponent, A chain
Gene symbol C1QA
Other names/aliases -
Species Homo sapiens
 Database cross references - C1QA
ExoCarta ExoCarta_712
Vesiclepedia VP_712
Entrez Gene 712
HGNC 1241
MIM 120550
UniProt P02745  
 C1QA identified in sEVs derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for C1QA
Molecular Function
    amyloid-beta binding GO:0001540 TAS
    protein binding GO:0005515 IPI
Biological Process
    microglial cell activation GO:0001774 ISS
    complement activation GO:0006956 TAS
    complement activation, classical pathway GO:0006958 IDA
    complement activation, classical pathway GO:0006958 NAS
    cell-cell signaling GO:0007267 TAS
    neuron remodeling GO:0016322 ISS
    innate immune response GO:0045087 IEA
    astrocyte activation GO:0048143 ISS
    synapse organization GO:0050808 ISS
    synapse pruning GO:0098883 ISS
    complement-mediated synapse pruning GO:0150062 ISS
    vertebrate eye-specific patterning GO:0150064 ISS
Subcellular Localization
    extracellular region GO:0005576 IDA
    extracellular region GO:0005576 NAS
    extracellular region GO:0005576 TAS
    collagen trimer GO:0005581 IEA
    complement component C1 complex GO:0005602 NAS
    synapse GO:0045202 ISS
    collagen-containing extracellular matrix GO:0062023 HDA
    complement component C1q complex GO:0062167 IPI
    postsynapse GO:0098794 ISS
    extrinsic component of presynaptic membrane GO:0098888 IEA
    extrinsic component of postsynaptic membrane GO:0098890 IEA
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified C1QA in sEVs
1
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for C1QA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UBE2H 7328
Affinity Capture-MS Homo sapiens
2 COLGALT2  
Affinity Capture-MS Homo sapiens
3 ACOX3 8310
Affinity Capture-MS Homo sapiens
4 NPTX1  
Affinity Capture-MS Homo sapiens
5 MAP2K6 5608
Affinity Capture-MS Homo sapiens
6 POGLUT1 56983
Affinity Capture-MS Homo sapiens
7 HSPB2  
Two-hybrid Homo sapiens
8 CNTNAP3  
Affinity Capture-MS Homo sapiens
9 GNPNAT1 64841
Two-hybrid Homo sapiens
10 HMGB1 3146
Two-hybrid Homo sapiens
11 DCN 1634
Reconstituted Complex Homo sapiens
12 CES3 23491
Affinity Capture-MS Homo sapiens
13 TUBB3 10381
Affinity Capture-MS Homo sapiens
14 DDX19B 11269
Affinity Capture-MS Homo sapiens
15 UBQLN2 29978
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
16 NLGN3  
Two-hybrid Homo sapiens
17 SLC25A47  
Two-hybrid Homo sapiens
18 HIPK3 10114
Two-hybrid Homo sapiens
19 PISD  
Affinity Capture-MS Homo sapiens
20 MRC2 9902
Affinity Capture-MS Homo sapiens
21 TUBA4A 7277
Affinity Capture-MS Homo sapiens
22 INSR 3643
Affinity Capture-MS Homo sapiens
23 ITGAV 3685
Affinity Capture-MS Homo sapiens
24 CTU2 348180
Affinity Capture-MS Homo sapiens
25 NELFA  
Affinity Capture-MS Homo sapiens
26 NRP2 8828
Affinity Capture-MS Homo sapiens
27 USP30  
Affinity Capture-MS Homo sapiens
28 FN1 2335
Reconstituted Complex Homo sapiens
29 BAG6 7917
Two-hybrid Homo sapiens
30 PPP1CC 5501
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
31 C1S 716
Affinity Capture-Western Homo sapiens
32 SGTB  
Two-hybrid Homo sapiens
33 MANBA 4126
Affinity Capture-MS Homo sapiens
34 TXN 7295
Reconstituted Complex Homo sapiens
35 PLOD2 5352
Affinity Capture-MS Homo sapiens
36 FUT11 170384
Affinity Capture-MS Homo sapiens
37 ITGA7 3679
Affinity Capture-MS Homo sapiens
38 PLOD3 8985
Affinity Capture-MS Homo sapiens
39 TMEM132A 54972
Affinity Capture-MS Homo sapiens
40 C1R 715
Affinity Capture-Western Homo sapiens
41 PIAS2  
Two-hybrid Homo sapiens
42 CRP  
Reconstituted Complex Homo sapiens
43 WNT5A 7474
Affinity Capture-MS Homo sapiens
44 TCTN2  
Affinity Capture-MS Homo sapiens
45 SUSD1 64420
Affinity Capture-MS Homo sapiens
46 RNASEL 6041
Affinity Capture-MS Homo sapiens
47 SEL1L 6400
Affinity Capture-MS Homo sapiens
48 PPP1CA 5499
Two-hybrid Homo sapiens
49 P3H3 10536
Affinity Capture-MS Homo sapiens
50 SGTA 6449
Two-hybrid Homo sapiens
51 RAC1 5879
Affinity Capture-Western Homo sapiens
Affinity Capture-Luminescence Homo sapiens
52 PLXNA2 5362
Affinity Capture-MS Homo sapiens
53 LOX 4015
Affinity Capture-MS Homo sapiens
54 UBQLN1 29979
Two-hybrid Homo sapiens
55 TUBB8 347688
Affinity Capture-MS Homo sapiens
56 ENPP1 5167
Affinity Capture-MS Homo sapiens
57 ITGA4 3676
Affinity Capture-MS Homo sapiens
58 APOA1 335
Affinity Capture-MS Homo sapiens
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