Gene ontology annotations for APOA1
Experiment description of studies that identified APOA1 in sEVs
1
Experiment ID
494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
Osteoarthritic cartilage
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
2
Experiment ID
496
MISEV standards
✘
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
Healthy cartilage
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
3
Experiment ID
497
MISEV standards
✘
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
4
Experiment ID
498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
5
Experiment ID
20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
17956143
Organism
Homo sapiens
Experiment description
Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors
"Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name
JPR
Publication year
2007
Sample
Colorectal cancer cells
Sample name
HT29
Isolation/purification methods
Differential centrifugation Sucrose density gradient Diafiltration
Flotation density
1.16 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
6
Experiment ID
285
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘
Negative markers
✔
DLS
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
7
Experiment ID
286
MISEV standards
✔
CEM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81|EpCAM
Enriched markers
✘
Negative markers
✔
DLS
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25890246
Organism
Homo sapiens
Experiment description
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors
"Xu R, Greening DW, Rai A, Ji H, Simpson RJ."
Journal name
Methods
Publication year
2015
Sample
Colorectal cancer cells
Sample name
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
8
Experiment ID
234
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocellular carcinoma cells
Sample name
HKCI-C3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RT-PCR RNA Sequencing
9
Experiment ID
237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocytes
Sample name
MIHA
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RNA Sequencing
10
Experiment ID
237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocytes
Sample name
MIHA
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RNA Sequencing
11
Experiment ID
266
MISEV standards
✘
Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25265333
Organism
Homo sapiens
Experiment description
Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors
"Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name
J Proteome Res
Publication year
2014
Sample
Liver cancer cells
Sample name
Huh-7 - Wild type
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
12
Experiment ID
267
MISEV standards
✘
Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25265333
Organism
Homo sapiens
Experiment description
Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors
"Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name
J Proteome Res
Publication year
2014
Sample
Liver cancer cells
Sample name
Huh-7 - Transfected with HBx plasmids
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
13
Experiment ID
268
MISEV standards
✘
Biophysical techniques
✔
TSG101|Alix
Enriched markers
✔
CANX|HSP90B1
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25265333
Organism
Homo sapiens
Experiment description
Quantitative Proteomic Analysis of Exosome Protein Content Changes Induced by Hepatitis B Virus in Huh-7 Cells Using SILAC Labeling and LC-MS/MS
Authors
"Zhao X, Wu Y, Duan J, Ma Y, Shen Z, Wei L, Cui X, Zhang J, Xie Y, Liu J"
Journal name
J Proteome Res
Publication year
2014
Sample
Liver cancer cells
Sample name
Huh-7 - Transfected with HBx-null HBV replicon plasmids
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
14
Experiment ID
254
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
MNT-1
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
15
Experiment ID
255
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
G1
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
16
Experiment ID
256
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
501mel
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
17
Experiment ID
257
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
Daju
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
18
Experiment ID
258
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
SKMEL28
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
19
Experiment ID
259
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
A375M
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
20
Experiment ID
260
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|FLOT1|CD81
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25950383
Organism
Homo sapiens
Experiment description
Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors
"Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L"
Journal name
Pigment Cell Melanoma Res
Publication year
2015
Sample
Melanoma cells
Sample name
1205Lu
Isolation/purification methods
Differential centrifugation Unltracentrifugation Sucrose density gradient
Flotation density
1.13 - 1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
21
Experiment ID
126
MISEV standards
✘
Biophysical techniques
✔
GAPDH
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [LTQ-FT Ultra]
PubMed ID
Unpublished / Not applicable
Organism
Homo sapiens
Experiment description
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors
"Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name
Mesenchymal Stem Cell Therapy
Publication year
2011
Sample
Mesenchymal stem cells
Sample name
huES9.E1
Isolation/purification methods
HPLC
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Antobody array Mass spectrometry
22
Experiment ID
488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Homo sapiens
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Mesenchymal stem cells
Sample name
UCMSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
23
Experiment ID
606
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|HSP90|TSG101|GAPDH
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Western blotting
PubMed ID
36624553
Organism
Homo sapiens
Experiment description
Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors
"Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L. "
Journal name
Proteomics
Publication year
2023
Sample
Plasma
Sample name
Plasma - Ion exchange adsorption 12.5 mg
Isolation/purification methods
Filtration Size exclusion chromatography Ion exchange adsorption Centrifugation Centrifugal ultrafiltration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
24
Experiment ID
606
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|HSP90|TSG101|GAPDH
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36624553
Organism
Homo sapiens
Experiment description
Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors
"Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L. "
Journal name
Proteomics
Publication year
2023
Sample
Plasma
Sample name
Plasma - Ion exchange adsorption 12.5 mg
Isolation/purification methods
Filtration Size exclusion chromatography Ion exchange adsorption Centrifugation Centrifugal ultrafiltration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
25
Experiment ID
607
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|HSP90|TSG101|GAPDH
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Western blotting
PubMed ID
36624553
Organism
Homo sapiens
Experiment description
Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors
"Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L. "
Journal name
Proteomics
Publication year
2023
Sample
Plasma
Sample name
Plasma - Ion exchange adsorption 25 mg
Isolation/purification methods
Filtration Size exclusion chromatography Ion exchange adsorption Centrifugation Centrifugal ultrafiltration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
26
Experiment ID
607
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|HSP90|TSG101|GAPDH
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36624553
Organism
Homo sapiens
Experiment description
Combination of size-exclusion chromatography and ion exchange adsorption for improving the proteomic analysis of plasma-derived extracellular vesicles
Authors
"Wang Y, Zhang Y, Li Z, Wei S, Chi X, Yan X, Lv H, Zhao L, Zhao L. "
Journal name
Proteomics
Publication year
2023
Sample
Plasma
Sample name
Plasma - Ion exchange adsorption 25 mg
Isolation/purification methods
Filtration Size exclusion chromatography Ion exchange adsorption Centrifugation Centrifugal ultrafiltration
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
27
Experiment ID
231
MISEV standards
✘
Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
28
Experiment ID
232
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
29
Experiment ID
233
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
30
Experiment ID
143
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
"Hosseini-Beheshti E, Guns ES."
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
LNCaP - Rep 3
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
31
Experiment ID
275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel sensitive
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.12-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry/Flow cytometry/Western blotting
32
Experiment ID
834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
33
Experiment ID
835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
34
Experiment ID
66
MISEV standards
✔
IEM
Biophysical techniques
✔
TSG101|Alix|CD63|CD81
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19199708
Organism
Homo sapiens
Experiment description
Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Authors
"Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR"
Journal name
JPR
Publication year
2009
Sample
Saliva
Sample name
Saliva
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting Immunoelectron Microscopy
35
Experiment ID
457
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|TSG101
Enriched markers
✔
CALR
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Western blotting
PubMed ID
36517516
Organism
Homo sapiens
Experiment description
Exosomal miR-1304-3p promotes breast cancer progression in African Americans by activating cancer-associated adipocytes
Authors
"Zhao D, Wu K, Sharma S, Xing F, Wu SY, Tyagi A, Deshpande R, Singh R, Wabitsch M, Mo YY, Watabe K."
Journal name
Nat Commun
Publication year
2022
Sample
Serum
Sample name
Breast cancer patients - African American
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting
36
Experiment ID
217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
37
Experiment ID
63
MISEV standards
✘
Biophysical techniques
✔
AQP2
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
19056867
Organism
Homo sapiens
Experiment description
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors
"Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name
JASN
Publication year
2009
Sample
Urine
Sample name
Urine - Normal
Isolation/purification methods
Differential centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
Protein-protein interactions for APOA1
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
MIS12
Affinity Capture-MS
Homo sapiens
2
SMPDL3A
10924
Affinity Capture-MS
Homo sapiens
3
KLHL28
Affinity Capture-MS
Homo sapiens
4
HBB
3043
Affinity Capture-MS
Homo sapiens
5
STATH
Affinity Capture-MS
Homo sapiens
6
KRT1
3848
Affinity Capture-MS
Homo sapiens
7
CMTM5
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
Two-hybrid
Homo sapiens
8
ABCA13
Reconstituted Complex
Homo sapiens
9
GJA1
2697
Affinity Capture-MS
Homo sapiens
10
TTR
7276
Affinity Capture-MS
Homo sapiens
11
Papss1
Affinity Capture-MS
Mus musculus
12
ALB
213
Affinity Capture-MS
Homo sapiens
13
ALDH2
217
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
14
ABCA1
19
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
15
SETX
Affinity Capture-MS
Homo sapiens
16
ITIH2
3698
Cross-Linking-MS (XL-MS)
Homo sapiens
17
TFPI
7035
Cross-Linking-MS (XL-MS)
Homo sapiens
18
KRT73
319101
Cross-Linking-MS (XL-MS)
Homo sapiens
19
HYPK
25764
Affinity Capture-MS
Homo sapiens
20
SOX2
Affinity Capture-MS
Homo sapiens
21
FNDC5
Affinity Capture-MS
Homo sapiens
22
APOD
347
Cross-Linking-MS (XL-MS)
Homo sapiens
23
HPX
3263
Affinity Capture-MS
Homo sapiens
24
APOC1
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
25
HP
3240
Protein-peptide
Homo sapiens
Affinity Capture-MS
Homo sapiens
26
CLEC4G
Two-hybrid
Homo sapiens
27
FGA
2243
Affinity Capture-MS
Homo sapiens
Co-localization
Homo sapiens
28
ABCA6
23460
Affinity Capture-MS
Homo sapiens
29
NDRG1
10397
Two-hybrid
Homo sapiens
FRET
Homo sapiens
30
APOC3
345
Cross-Linking-MS (XL-MS)
Homo sapiens
Affinity Capture-MS
Homo sapiens
31
TLN1
7094
Cross-Linking-MS (XL-MS)
Homo sapiens
32
EGFL8
Affinity Capture-MS
Homo sapiens
33
VKORC1
79001
Two-hybrid
Homo sapiens
34
OR13C3
Affinity Capture-MS
Homo sapiens
35
GPLD1
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
36
SFXN4
Affinity Capture-MS
Homo sapiens
37
SCARB1
949
Reconstituted Complex
Homo sapiens
38
UCHL5
51377
Affinity Capture-MS
Homo sapiens
39
PHF11
51131
Affinity Capture-MS
Homo sapiens
40
VCAM1
7412
Affinity Capture-MS
Homo sapiens
41
FNDC1
84624
Affinity Capture-MS
Homo sapiens
42
PDE1A
Two-hybrid
Homo sapiens
43
MYH6
4624
Cross-Linking-MS (XL-MS)
Homo sapiens
44
SPEF2
79925
Affinity Capture-MS
Homo sapiens
45
SERPINA1
5265
Affinity Capture-MS
Homo sapiens
46
SNX27
81609
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
47
PCYOX1
51449
Cross-Linking-MS (XL-MS)
Homo sapiens
48
APOC2
344
Affinity Capture-MS
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
49
DDX19B
11269
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
50
DDX31
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
51
GNG8
Affinity Capture-MS
Homo sapiens
52
C1QC
714
Affinity Capture-MS
Homo sapiens
53
FBLN1
2192
Two-hybrid
Homo sapiens
54
ST6GALNAC6
30815
Affinity Capture-MS
Homo sapiens
55
WHSC1
7468
Affinity Capture-MS
Homo sapiens
56
CLU
1191
Affinity Capture-MS
Homo sapiens
57
ROBO3
Affinity Capture-MS
Homo sapiens
58
A2M
2
Cross-Linking-MS (XL-MS)
Homo sapiens
59
GOLGA8A
23015
Affinity Capture-MS
Homo sapiens
60
AHNAK
79026
Cross-Linking-MS (XL-MS)
Homo sapiens
61
TMEM43
79188
Affinity Capture-MS
Homo sapiens
62
C1QA
712
Affinity Capture-MS
Homo sapiens
63
VCP
7415
Affinity Capture-Western
Homo sapiens
64
CFAP54
Cross-Linking-MS (XL-MS)
Homo sapiens
65
APOA2
336
Affinity Capture-MS
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
66
KRT9
3857
Affinity Capture-MS
Homo sapiens
67
U2AF2
11338
Affinity Capture-MS
Homo sapiens
68
TOMM20
9804
Two-hybrid
Homo sapiens
69
APOL1
8542
Co-purification
Homo sapiens
70
PRR4
11272
Affinity Capture-MS
Homo sapiens
71
GDPD1
284161
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
72
ITGB2
3689
Cross-Linking-MS (XL-MS)
Homo sapiens
73
TNFRSF10C
Two-hybrid
Homo sapiens
74
LCN1
3933
Affinity Capture-MS
Homo sapiens
75
ATP4A
495
Affinity Capture-MS
Homo sapiens
76
RRP1B
23076
Affinity Capture-MS
Homo sapiens
77
BRAP
Two-hybrid
Homo sapiens
78
DDX39B
7919
Affinity Capture-MS
Homo sapiens
79
SAFB2
9667
Affinity Capture-MS
Homo sapiens
80
SERPINA3
12
Cross-Linking-MS (XL-MS)
Homo sapiens
81
IGKC
3514
Affinity Capture-MS
Homo sapiens
82
HBA1
3039
Affinity Capture-MS
Homo sapiens
83
KIAA0754
Affinity Capture-MS
Homo sapiens
84
FAM83C
Affinity Capture-MS
Homo sapiens
85
APOF
319
Co-purification
Homo sapiens
86
SARAF
Affinity Capture-MS
Homo sapiens
87
KRT3
3850
Cross-Linking-MS (XL-MS)
Homo sapiens
88
PPP2R2B
5521
Affinity Capture-MS
Homo sapiens
89
FANCD2
Affinity Capture-MS
Homo sapiens
90
HMGCLL1
Affinity Capture-MS
Homo sapiens
91
AGPAT1
10554
Affinity Capture-MS
Homo sapiens
92
ITLN2
Affinity Capture-MS
Homo sapiens
93
APOB
338
Affinity Capture-Western
Homo sapiens
Affinity Capture-Western
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
94
QDPR
5860
Affinity Capture-MS
Homo sapiens
95
CIR1
Affinity Capture-MS
Homo sapiens
96
NR3C1
2908
Affinity Capture-MS
Homo sapiens
97
CP
1356
Affinity Capture-MS
Homo sapiens
98
UPF3A
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
99
TNS3
64759
Affinity Capture-MS
Homo sapiens
100
FN1
2335
Affinity Capture-MS
Homo sapiens
101
MAP7D1
55700
Two-hybrid
Homo sapiens
102
MAPK6
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
103
ZNF558
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
104
C6
729
Cross-Linking-MS (XL-MS)
Homo sapiens
105
GOT1L1
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
106
NLRP1
Affinity Capture-MS
Homo sapiens
107
AKT2
208
Two-hybrid
Homo sapiens
108
CD40LG
959
Affinity Capture-Western
Homo sapiens
109
FAM169A
Affinity Capture-MS
Homo sapiens
110
MYH7
4625
Cross-Linking-MS (XL-MS)
Homo sapiens
111
MSN
4478
Cross-Linking-MS (XL-MS)
Homo sapiens
112
Lep
Reconstituted Complex
Mus musculus
113
STX17
55014
Affinity Capture-MS
Homo sapiens
114
KRT16
3868
Affinity Capture-MS
Homo sapiens
115
UBE2U
Affinity Capture-MS
Homo sapiens
116
APP
351
Reconstituted Complex
Homo sapiens
Affinity Capture-Western
Homo sapiens
Reconstituted Complex
Homo sapiens
117
ORM1
5004
Affinity Capture-MS
Homo sapiens
118
APOA1BP
128240
Affinity Capture-Western
Homo sapiens
119
SNCA
6622
Affinity Capture-MS
Homo sapiens
120
CTSL
1514
Affinity Capture-MS
Homo sapiens
121
PDE4B
Affinity Capture-MS
Homo sapiens
122
SCGB1D1
Affinity Capture-MS
Homo sapiens
123
CNN1
1264
Cross-Linking-MS (XL-MS)
Homo sapiens
124
MOCOS
Affinity Capture-MS
Homo sapiens
125
SPP1
6696
Two-hybrid
Homo sapiens
126
LECT1
Affinity Capture-MS
Homo sapiens
127
SAA1
6288
Cross-Linking-MS (XL-MS)
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
128
LGALS3
3958
Affinity Capture-MS
Homo sapiens
129
Cep135
Affinity Capture-MS
Mus musculus
130
LCAT
3931
Biochemical Activity
Homo sapiens
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
Reconstituted Complex
Homo sapiens
131
ERVK-10
Affinity Capture-MS
Homo sapiens
132
KRT71
112802
Cross-Linking-MS (XL-MS)
Homo sapiens
133
PLTP
5360
Reconstituted Complex
Homo sapiens
Co-purification
Homo sapiens
134
PDE6D
5147
Affinity Capture-MS
Homo sapiens
135
TF
7018
Affinity Capture-MS
Homo sapiens
136
JAK3
3718
Two-hybrid
Homo sapiens
137
PTPN5
Affinity Capture-MS
Homo sapiens
138
MBNL1
4154
Affinity Capture-MS
Homo sapiens
139
FBLN7
Affinity Capture-MS
Homo sapiens
140
KLK10
5655
Affinity Capture-MS
Homo sapiens
141
DNTT
1791
Affinity Capture-MS
Homo sapiens
142
FLNA
2316
Cross-Linking-MS (XL-MS)
Homo sapiens
143
GOT1
2805
Affinity Capture-MS
Homo sapiens
144
SAA2
6289
Cross-Linking-MS (XL-MS)
Homo sapiens
Two-hybrid
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
145
ZNRD1
Two-hybrid
Homo sapiens
146
CUL7
9820
Affinity Capture-MS
Homo sapiens
147
DTWD1
Affinity Capture-MS
Homo sapiens
148
IGKV2-40
Affinity Capture-MS
Homo sapiens
149
SAA4
6291
Cross-Linking-MS (XL-MS)
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
150
MATN2
4147
Affinity Capture-MS
Homo sapiens
151
CCR1
Affinity Capture-MS
Homo sapiens
152
TXNDC2
Affinity Capture-MS
Homo sapiens
153
P2RX6
Affinity Capture-MS
Homo sapiens
154
LBP
3929
Affinity Capture-Western
Homo sapiens
155
SERTAD3
Affinity Capture-MS
Homo sapiens
156
APOA1
335
Co-crystal Structure
Homo sapiens
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
Co-crystal Structure
Homo sapiens
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
157
PLXDC1
Cross-Linking-MS (XL-MS)
Homo sapiens
View the network
image/svg+xml
Pathways in which APOA1 is involved