Gene description for APOD
Gene name apolipoprotein D
Gene symbol APOD
Other names/aliases -
Species Homo sapiens
 Database cross references - APOD
ExoCarta ExoCarta_347
Entrez Gene 347
HGNC 612
MIM 107740
UniProt P05090  
 APOD identified in exosomes derived from the following tissue/cell type
Plasma 19028452    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Urine 15326289    
Urine 19056867    
Urine 21595033    
Urine 21595033    
Urine 21595033    
Urine 22418980    
Urine 22418980    
Urine 25452312    
 Gene ontology annotations for APOD
Molecular Function
    cholesterol binding GO:0015485 IDA
    protein binding GO:0005515 IPI
    lipid transporter activity GO:0005319 NAS
Biological Process
    negative regulation of smooth muscle cell proliferation GO:0048662 IDA
    negative regulation of platelet-derived growth factor receptor signaling pathway GO:0010642 IDA
    negative regulation of monocyte chemotactic protein-1 production GO:0071638 IDA
    tissue regeneration GO:0042246 ISS
    lipid transport GO:0006869 NAS
    negative regulation of protein import into nucleus GO:0042308 IDA
    response to reactive oxygen species GO:0000302 IDA
    negative regulation of focal adhesion assembly GO:0051895 IMP
    angiogenesis GO:0001525 NAS
    lipid metabolic process GO:0006629 IDA
    brain development GO:0007420 ISS
    negative regulation of T cell migration GO:2000405 IDA
    response to drug GO:0042493 ISS
    response to axon injury GO:0048678 ISS
    peripheral nervous system axon regeneration GO:0014012 ISS
    aging GO:0007568 NAS
    negative regulation of cytokine production involved in inflammatory response GO:1900016 IDA
    glucose metabolic process GO:0006006 IDA
    negative regulation of smooth muscle cell-matrix adhesion GO:2000098 IMP
    negative regulation of lipoprotein lipid oxidation GO:0060588 IDA
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IDA
    extracellular space GO:0005615 IDA
    cytosolic ribosome GO:0022626 ISS
    endoplasmic reticulum GO:0005783 ISS
    extracellular region GO:0005576 NAS
    neuronal cell body GO:0043025 ISS
    extracellular exosome GO:0070062 IDA
    dendrite GO:0030425 ISS
 Experiment description of studies that identified APOD in exosomes
1
Experiment ID 44
ISEV standards
IEM
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19028452    
Organism Homo sapiens
Experiment description Proteomic profiling of human plasma exosomes identifies PPARgamma as an exosome-associated protein.
Authors Looze C, Yui D, Leung L, Ingham M, Kaler M, Yao X, Wu WW, Shen RF, Daniels MP, Levine SJ
Journal name BBRC
Publication year 2009
Sample Plasma
Sample name Plasma
Isolation/purification methods Filtration
Size exclusion
Sucrose density gradient
Flotation density 1.15-1.16 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
Immunoelectron Microscopy
2
Experiment ID 231
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD63|CD9
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 13
ISEV standards
IEM
EV Biophysical techniques
Alix|RAB4|RAB5B|RAB11|TSG101
EV Cytosolic markers
CD9|AQP2|AQP1
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 15326289    
Organism Homo sapiens
Experiment description Identification and proteomic profiling of exosomes in human urine.
Authors Pisitkun T, Shen RF, Knepper MA
Journal name PNAS
Publication year 2004
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA XP]
Western blotting
6
Experiment ID 63
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
AQP2
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
7
Experiment ID 193
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63|CD9
EV Membrane markers
PHB
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 194
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of basement membrane nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 195
ISEV standards
EV Biophysical techniques
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 21595033    
Organism Homo sapiens
Experiment description Proteomic analysis of urinary exosomes from patients of early IgA nephropathy and thin basement membrane nephropathy.
Authors Moon PG, Lee JE, You S, Kim TK, Cho JH, Kim IS, Kwon TH, Kim CD, Park SH, Hwang D, Kim YL, Baek MC.
Journal name Proteomics
Publication year 2011
Sample Urine
Sample name Urine - Patients of early IgA nephropathy
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 196
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 197
ISEV standards
EM
EV Biophysical techniques
Alix|TSG101|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
12
Experiment ID 238
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25452312    
Organism Homo sapiens
Experiment description N-linked (N-) glycoproteomics of urimary exosomes
Authors Saraswat M, Joenvaara S, Musante L, Peltoniemi H4, Holthofer H, Renkonen R.
Journal name Mol Cell Proteomics
Publication year 2014
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Filtration
Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for APOD
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APOD 347
Co-purification Homo sapiens
Co-purification Homo sapiens
2 STK24 8428
Affinity Capture-MS Homo sapiens
3 GRINL1A  
Two-hybrid Homo sapiens
4 APOA2 336
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
View the network image/svg+xml
 Pathways in which APOD is involved
PathwayEvidenceSource
Transport of fatty acids TAS Reactome





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