Gene description for MFF
Gene name mitochondrial fission factor
Gene symbol MFF
Other names/aliases C2orf33
GL004
Species Homo sapiens
 Database cross references - MFF
ExoCarta ExoCarta_56947
Vesiclepedia VP_56947
Entrez Gene 56947
HGNC 24858
MIM 614785
UniProt Q9GZY8  
 MFF identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for MFF
Molecular Function
    protein binding GO:0005515 IPI
    identical protein binding GO:0042802 IPI
    protein homodimerization activity GO:0042803 IDA
Biological Process
    mitochondrial fission GO:0000266 IDA
    mitochondrial fission GO:0000266 IMP
    mitochondrial fission GO:0000266 ISS
    protein targeting to mitochondrion GO:0006626 IBA
    protein targeting to mitochondrion GO:0006626 IDA
    protein targeting to mitochondrion GO:0006626 IMP
    protein targeting to mitochondrion GO:0006626 ISS
    mitochondrion organization GO:0007005 IMP
    peroxisome fission GO:0016559 IMP
    positive regulation of mitochondrial fission GO:0090141 IBA
Subcellular Localization
    mitochondrion GO:0005739 HTP
    mitochondrion GO:0005739 IDA
    mitochondrial outer membrane GO:0005741 IBA
    mitochondrial outer membrane GO:0005741 IDA
    peroxisome GO:0005777 IBA
    peroxisome GO:0005777 IDA
    synaptic vesicle GO:0008021 IEA
    mitochondrial membrane GO:0031966 IDA
    protein-containing complex GO:0032991 IDA
 Experiment description of studies that identified MFF in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for MFF
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SEC22B 9554
Two-hybrid Homo sapiens
2 LOC100507537  
Two-hybrid Homo sapiens
3 CCDC126 90693
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MTCH1 23787
Proximity Label-MS Homo sapiens
5 SLC18A3  
Two-hybrid Homo sapiens
6 SACM1L 22908
Two-hybrid Homo sapiens
7 TMEM88  
Two-hybrid Homo sapiens
8 TUSC5  
Two-hybrid Homo sapiens
9 SMIM1 388588
Two-hybrid Homo sapiens
10 AIG1  
Two-hybrid Homo sapiens
11 GOSR2 9570
Two-hybrid Homo sapiens
12 CD79A  
Two-hybrid Homo sapiens
13 PTPN1 5770
Proximity Label-MS Homo sapiens
14 TMEM86B  
Two-hybrid Homo sapiens
15 APOD 347
Two-hybrid Homo sapiens
16 PDE2A  
Affinity Capture-MS Homo sapiens
17 SERINC2 347735
Two-hybrid Homo sapiens
18 OPN1SW  
Two-hybrid Homo sapiens
19 TEX264 51368
Two-hybrid Homo sapiens
20 FIS1 51024
Proximity Label-MS Homo sapiens
21 GKN1  
Two-hybrid Homo sapiens
22 FBXW7  
Affinity Capture-MS Homo sapiens
23 STX8 9482
Two-hybrid Homo sapiens
24 OPRM1 4988
Two-hybrid Homo sapiens
25 C14orf180  
Two-hybrid Homo sapiens
26 MARCH5  
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 TMEM201 199953
Two-hybrid Homo sapiens
28 TSPO2  
Two-hybrid Homo sapiens
29 LPAR3  
Two-hybrid Homo sapiens
30 C3orf52  
Two-hybrid Homo sapiens
31 EXD2  
Proximity Label-MS Homo sapiens
32 TMEM140  
Two-hybrid Homo sapiens
33 SLC25A46 91137
Proximity Label-MS Homo sapiens
34 SEC11C 90701
Two-hybrid Homo sapiens
35 BET1 10282
Two-hybrid Homo sapiens
36 SEC23A 10484
Two-hybrid Homo sapiens
37 SNX27 81609
Affinity Capture-MS Homo sapiens
38 CEND1  
Two-hybrid Homo sapiens
39 MOSPD3  
Two-hybrid Homo sapiens
40 SPATA19  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 TMEM42  
Two-hybrid Homo sapiens
42 SLC35B1 10237
Two-hybrid Homo sapiens
43 TMEM254  
Two-hybrid Homo sapiens
44 MTNR1B  
Two-hybrid Homo sapiens
45 PXMP2  
Proximity Label-MS Homo sapiens
46 FCER1G 2207
Two-hybrid Homo sapiens
47 MTCH2 23788
Proximity Label-MS Homo sapiens
48 SAMM50 25813
Proximity Label-MS Homo sapiens
49 TSPAN33 340348
Two-hybrid Homo sapiens
50 IGFBP5 3488
Two-hybrid Homo sapiens
51 JAGN1 84522
Two-hybrid Homo sapiens
52 PSMD14 10213
Affinity Capture-MS Homo sapiens
53 TSPAN2 10100
Two-hybrid Homo sapiens
54 RAET1L  
Two-hybrid Homo sapiens
55 APOL2 23780
Two-hybrid Homo sapiens
56 SLC41A2 84102
Two-hybrid Homo sapiens
57 TMEM97 27346
Two-hybrid Homo sapiens
58 RHBDL1  
Two-hybrid Homo sapiens
59 MEOX2  
Two-hybrid Homo sapiens
60 YWHAQ 10971
Affinity Capture-MS Homo sapiens
61 CLDN19  
Two-hybrid Homo sapiens
62 RHOT2 89941
Proximity Label-MS Homo sapiens
63 PLK3  
Two-hybrid Homo sapiens
64 MFF 56947
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
65 DNM1L 10059
Proximity Label-MS Homo sapiens
66 RMDN3 55177
Proximity Label-MS Homo sapiens
67 TRAM1L1  
Two-hybrid Homo sapiens
68 CDIPT 10423
Two-hybrid Homo sapiens
69 TMEM60  
Two-hybrid Homo sapiens
70 DUSP22  
Affinity Capture-MS Homo sapiens
71 TMEM14C 51522
Two-hybrid Homo sapiens
72 PLN  
Two-hybrid Homo sapiens
73 LAT 27040
Two-hybrid Homo sapiens
74 CYB5B 80777
Two-hybrid Homo sapiens
75 UPK1B 7348
Two-hybrid Homo sapiens
76 AIFM1 9131
Proximity Label-MS Homo sapiens
77 ZFPL1 7542
Two-hybrid Homo sapiens
78 BAG2 9532
Affinity Capture-MS Homo sapiens
79 MTX2 10651
Proximity Label-MS Homo sapiens
80 ERGIC3 51614
Two-hybrid Homo sapiens
81 TMEM128  
Two-hybrid Homo sapiens
82 YWHAZ 7534
Affinity Capture-MS Homo sapiens
83 FXYD6  
Two-hybrid Homo sapiens
84 NRM 11270
Two-hybrid Homo sapiens
85 MAVS 57506
Proximity Label-MS Homo sapiens
86 FKBP8 23770
Proximity Label-MS Homo sapiens
87 AQP3  
Two-hybrid Homo sapiens
88 DUSP16  
Affinity Capture-MS Homo sapiens
89 PLLP  
Two-hybrid Homo sapiens
90 OCIAD1 54940
Proximity Label-MS Homo sapiens
91 GJB1 2705
Two-hybrid Homo sapiens
92 GPR152  
Two-hybrid Homo sapiens
93 FAM209A 200232
Two-hybrid Homo sapiens
94 MAPK6  
Affinity Capture-MS Homo sapiens
95 FAM134C 162427
Two-hybrid Homo sapiens
96 TMEM229B  
Two-hybrid Homo sapiens
97 TMEM237 65062
Two-hybrid Homo sapiens
98 RPA3 6119
Proximity Label-MS Homo sapiens
99 COL4A5  
Two-hybrid Homo sapiens
100 CMTM5  
Two-hybrid Homo sapiens
101 MPZL1 9019
Two-hybrid Homo sapiens
102 POTEKP 440915
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 CTXN3  
Two-hybrid Homo sapiens
104 HAX1  
Proximity Label-MS Homo sapiens
105 MIP  
Two-hybrid Homo sapiens
106 FATE1  
Two-hybrid Homo sapiens
107 PEX3 8504
Proximity Label-MS Homo sapiens
108 AKAP1 8165
Proximity Label-MS Homo sapiens
109 TMX2 51075
Two-hybrid Homo sapiens
110 AQP9  
Affinity Capture-MS Homo sapiens
111 FDCSP  
Two-hybrid Homo sapiens
112 UNC50  
Two-hybrid Homo sapiens
113 ATP6V0B 533
Two-hybrid Homo sapiens
114 PRKY  
Affinity Capture-MS Homo sapiens
115 PLP1 5354
Two-hybrid Homo sapiens
116 ILF3 3609
Co-fractionation Homo sapiens
117 TMEM218  
Two-hybrid Homo sapiens
118 TNFSF14  
Affinity Capture-MS Homo sapiens
119 NTS  
Two-hybrid Homo sapiens
120 FAM3C 10447
Two-hybrid Homo sapiens
121 VAMP4 8674
Two-hybrid Homo sapiens
122 MGAT5 4249
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 UNC93B1 81622
Two-hybrid Homo sapiens
124 LHFPL5  
Two-hybrid Homo sapiens
125 C17orf62 79415
Two-hybrid Homo sapiens
126 MMGT1 93380
Two-hybrid Homo sapiens
127 ADIPOQ 9370
Two-hybrid Homo sapiens
128 TM6SF1  
Two-hybrid Homo sapiens
129 BCL2  
Affinity Capture-MS Homo sapiens
130 C1QL4  
Two-hybrid Homo sapiens
131 RAB7A 7879
Proximity Label-MS Homo sapiens
132 SEC22A  
Two-hybrid Homo sapiens
133 FAM210B  
Two-hybrid Homo sapiens
134 SLC7A1 6541
Two-hybrid Homo sapiens
135 ANKRD46 157567
Two-hybrid Homo sapiens
136 ASB14  
Affinity Capture-MS Homo sapiens
137 DDX28  
Co-fractionation Homo sapiens
138 KIAA1644  
Two-hybrid Homo sapiens
139 CXCL16 58191
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MFF is involved
No pathways found





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