Gene description for TSPAN33
Gene name tetraspanin 33
Gene symbol TSPAN33
Other names/aliases PEN
PEN.
TSPAN-33
Species Homo sapiens
 Database cross references - TSPAN33
ExoCarta ExoCarta_340348
Vesiclepedia VP_340348
Entrez Gene 340348
HGNC 28743
MIM 610120
UniProt Q86UF1  
 TSPAN33 identified in exosomes derived from the following tissue/cell type
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for TSPAN33
Molecular Function
    protein binding GO:0005515 IPI
    enzyme binding GO:0019899 IPI
Biological Process
    pore complex assembly GO:0046931 IMP
    protein maturation GO:0051604 IBA
    protein maturation GO:0051604 IDA
    protein localization to plasma membrane GO:0072659 IBA
    protein localization to plasma membrane GO:0072659 IDA
Subcellular Localization
    endoplasmic reticulum lumen GO:0005788 TAS
    plasma membrane GO:0005886 IBA
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    adherens junction GO:0005912 IEA
    cell surface GO:0009986 IEA
    pore complex GO:0046930 IMP
    tetraspanin-enriched microdomain GO:0097197 IEA
 Experiment description of studies that identified TSPAN33 in exosomes
1
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TSPAN33
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CD99L2 83692
Affinity Capture-MS Homo sapiens
2 ADAM10 102
Affinity Capture-Western Homo sapiens
3 CLDN7 1366
Two-hybrid Homo sapiens
4 CKM  
Affinity Capture-MS Homo sapiens
5 GJA8  
Two-hybrid Homo sapiens
6 MYADM 91663
Affinity Capture-MS Homo sapiens
7 GPX8 493869
Two-hybrid Homo sapiens
8 SHISA4 149345
Two-hybrid Homo sapiens
9 GAD2  
Two-hybrid Homo sapiens
10 MYH13 8735
Affinity Capture-MS Homo sapiens
11 CKMT2  
Affinity Capture-MS Homo sapiens
12 HBA2 3040
Affinity Capture-MS Homo sapiens
13 MYH8 4626
Affinity Capture-MS Homo sapiens
14 TMEM106C 79022
Two-hybrid Homo sapiens
15 MTIF3  
Two-hybrid Homo sapiens
16 CRYAB 1410
Affinity Capture-MS Homo sapiens
17 EZR 7430
Affinity Capture-MS Homo sapiens
18 DPY19L3  
Affinity Capture-MS Homo sapiens
19 VKORC1L1 154807
Affinity Capture-MS Homo sapiens
20 SPINT1 6692
Two-hybrid Homo sapiens
21 ERGIC3 51614
Two-hybrid Homo sapiens
22 DES 1674
Affinity Capture-MS Homo sapiens
23 NEB 4703
Affinity Capture-MS Homo sapiens
24 RELL2  
Two-hybrid Homo sapiens
25 MYL1 4632
Affinity Capture-MS Homo sapiens
26 MYOM2 9172
Affinity Capture-MS Homo sapiens
27 CHRM2  
Two-hybrid Homo sapiens
28 ACTN3 89
Affinity Capture-MS Homo sapiens
29 TMEM194A  
Two-hybrid Homo sapiens
30 PLEKHA7 144100
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 MYH1  
Affinity Capture-MS Homo sapiens
32 MYLPF 29895
Affinity Capture-MS Homo sapiens
33 GORAB 92344
Two-hybrid Homo sapiens
34 FAM209A 200232
Two-hybrid Homo sapiens
35 MYBPC2 4606
Affinity Capture-MS Homo sapiens
36 PDZD11 51248
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 PYGM 5837
Affinity Capture-MS Homo sapiens
38 GPR152  
Two-hybrid Homo sapiens
39 LDB3  
Affinity Capture-MS Homo sapiens
40 ATP2A1 487
Affinity Capture-MS Homo sapiens
41 TTN 7273
Affinity Capture-MS Homo sapiens
42 DDR1 780
Affinity Capture-MS Homo sapiens
43 TNNC2  
Affinity Capture-MS Homo sapiens
44 KCNK5  
Two-hybrid Homo sapiens
45 TMEM45B 120224
Two-hybrid Homo sapiens
46 MYBPC1  
Affinity Capture-MS Homo sapiens
47 MYOM1  
Affinity Capture-MS Homo sapiens
48 SIT1 27240
Two-hybrid Homo sapiens
49 MYH7 4625
Affinity Capture-MS Homo sapiens
50 CISD2 493856
Two-hybrid Homo sapiens
51 MSN 4478
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 TNNT3  
Affinity Capture-MS Homo sapiens
53 LHFPL5  
Two-hybrid Homo sapiens
54 MFF 56947
Two-hybrid Homo sapiens
55 ANKRD13A 88455
Affinity Capture-MS Homo sapiens
56 MGST3 4259
Two-hybrid Homo sapiens
57 MYH4  
Affinity Capture-MS Homo sapiens
58 DARS2 55157
Two-hybrid Homo sapiens
59 ACTA1 58
Affinity Capture-MS Homo sapiens
60 MYH2  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which TSPAN33 is involved
PathwayEvidenceSource
Amyloid fiber formation TAS Reactome
Metabolism of proteins TAS Reactome





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