Gene description for SPINT1
Gene name serine peptidase inhibitor, Kunitz type 1
Gene symbol SPINT1
Other names/aliases HAI
HAI1
MANSC2
Species Homo sapiens
 Database cross references - SPINT1
ExoCarta ExoCarta_6692
Vesiclepedia VP_6692
Entrez Gene 6692
HGNC 11246
MIM 605123
UniProt O43278  
 SPINT1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 34887515    
Hepatocytes 26054723    
Prostate cancer cells 25844599    
 Gene ontology annotations for SPINT1
Molecular Function
    serine-type endopeptidase inhibitor activity GO:0004867 IBA
    protein binding GO:0005515 IPI
Biological Process
    neural tube closure GO:0001843 IEA
    epidermis development GO:0008544 IBA
    extracellular matrix organization GO:0030198 IBA
    positive regulation of glial cell differentiation GO:0045687 IEA
    epithelium development GO:0060429 IBA
    branching involved in labyrinthine layer morphogenesis GO:0060670 IEA
    placenta blood vessel development GO:0060674 IEA
    cellular response to BMP stimulus GO:0071773 IEA
    negative regulation of neural precursor cell proliferation GO:2000178 IEA
Subcellular Localization
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    cytoplasm GO:0005737 IEA
    plasma membrane GO:0005886 IBA
    membrane GO:0016020 TAS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified SPINT1 in exosomes
1
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
2
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
3
Experiment ID 275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors "Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
 Protein-protein interactions for SPINT1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LY86  
Affinity Capture-MS Homo sapiens
2 NRG1 3084
Affinity Capture-MS Homo sapiens
3 UBQLN2 29978
Two-hybrid Homo sapiens
4 ELSPBP1  
Affinity Capture-MS Homo sapiens
5 TMEM106A  
Affinity Capture-MS Homo sapiens
6 NAAA  
Affinity Capture-MS Homo sapiens
7 KLK15  
Affinity Capture-MS Homo sapiens
8 UPK1B 7348
Two-hybrid Homo sapiens
9 KRTAP1-3  
Two-hybrid Homo sapiens
10 HLA-G 3135
Affinity Capture-MS Homo sapiens
11 LDLRAD1  
Affinity Capture-MS Homo sapiens
12 ADAM33 80332
Two-hybrid Homo sapiens
13 NLGN3  
Two-hybrid Homo sapiens
14 CFC1  
Affinity Capture-MS Homo sapiens
15 FAM3C 10447
Two-hybrid Homo sapiens
16 PRG2  
Affinity Capture-MS Homo sapiens
17 TSPAN33 340348
Two-hybrid Homo sapiens
18 MAPK12  
Co-fractionation Homo sapiens
19 ZNF250  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 FOXK1 221937
Affinity Capture-MS Homo sapiens
21 AQP3  
Affinity Capture-MS Homo sapiens
22 SLC38A7 55238
Two-hybrid Homo sapiens
23 CLEC7A  
Two-hybrid Homo sapiens
24 MAL  
Two-hybrid Homo sapiens
25 VSIG4  
Affinity Capture-MS Homo sapiens
26 GZMH  
Affinity Capture-MS Homo sapiens
27 AGR3 155465
Two-hybrid Homo sapiens
28 TM2D3  
Affinity Capture-MS Homo sapiens
29 BRICD5  
Affinity Capture-MS Homo sapiens
30 SDF2L1 23753
Affinity Capture-MS Homo sapiens
31 FBXO2 26232
Affinity Capture-MS Homo sapiens
32 FOXK2  
Affinity Capture-MS Homo sapiens
33 TMEM190  
Two-hybrid Homo sapiens
34 TMEM109 79073
Two-hybrid Homo sapiens
35 SMCO4  
Two-hybrid Homo sapiens
36 FAM46A  
Two-hybrid Homo sapiens
37 UBQLN4 56893
Two-hybrid Homo sapiens
38 GXYLT1 283464
Affinity Capture-MS Homo sapiens
39 CST11  
Affinity Capture-MS Homo sapiens
40 CLEC2B  
Affinity Capture-MS Homo sapiens
41 ST8SIA5  
Affinity Capture-MS Homo sapiens
42 ST14 6768
Affinity Capture-MS Homo sapiens
43 HGFAC 3083
Affinity Capture-MS Homo sapiens
44 LYPD4  
Affinity Capture-MS Homo sapiens
45 TMEM97 27346
Two-hybrid Homo sapiens
46 UBXN6 80700
Co-fractionation Homo sapiens
47 VAMP5 10791
Two-hybrid Homo sapiens
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