Gene description for SPINT1
Gene name
serine peptidase inhibitor, Kunitz type 1
Gene symbol
SPINT1
Other names/aliases
HAI HAI1 MANSC2
Species
Homo sapiens
Database cross references - SPINT1
ExoCarta
ExoCarta_6692
Vesiclepedia
VP_6692
Entrez Gene
6692
HGNC
11246
MIM
605123
UniProt
O43278
SPINT1 identified in sEVs derived from the following tissue/cell type
Colorectal cancer cells
34887515
Hepatocytes
26054723
Prostate cancer cells
25844599
Gene ontology annotations for SPINT1
Experiment description of studies that identified SPINT1 in sEVs
1
Experiment ID
1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
34887515
Organism
Homo sapiens
Experiment description
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Colorectal cancer cells
Sample name
DiFi
Isolation/purification methods
Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting Mass spectrometry RNA sequencing
2
Experiment ID
237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule
mRNA
Identification method
RNA Sequencing
PubMed ID
26054723
Organism
Homo sapiens
Experiment description
Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors
"He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name
Carcinogenesis
Publication year
2015
Sample
Hepatocytes
Sample name
MIHA
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.19 g/mL
Molecules identified in the study
Protein RNA
Methods used in the study
Western blotting Mass spectrometry RNA Sequencing
3
Experiment ID
275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel sensitive
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.12-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry/Flow cytometry/Western blotting
Protein-protein interactions for SPINT1
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
LY86
Affinity Capture-MS
Homo sapiens
2
NRG1
3084
Affinity Capture-MS
Homo sapiens
3
UBQLN2
29978
Two-hybrid
Homo sapiens
4
ELSPBP1
Affinity Capture-MS
Homo sapiens
5
TMEM106A
Affinity Capture-MS
Homo sapiens
6
NAAA
Affinity Capture-MS
Homo sapiens
7
KLK15
Affinity Capture-MS
Homo sapiens
8
UPK1B
7348
Two-hybrid
Homo sapiens
9
KRTAP1-3
Two-hybrid
Homo sapiens
10
HLA-G
3135
Affinity Capture-MS
Homo sapiens
11
LDLRAD1
Affinity Capture-MS
Homo sapiens
12
ADAM33
80332
Two-hybrid
Homo sapiens
13
NLGN3
Two-hybrid
Homo sapiens
14
CFC1
Affinity Capture-MS
Homo sapiens
15
FAM3C
10447
Two-hybrid
Homo sapiens
16
PRG2
Affinity Capture-MS
Homo sapiens
17
TSPAN33
340348
Two-hybrid
Homo sapiens
18
MAPK12
Co-fractionation
Homo sapiens
19
ZNF250
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
20
FOXK1
221937
Affinity Capture-MS
Homo sapiens
21
AQP3
Affinity Capture-MS
Homo sapiens
22
SLC38A7
55238
Two-hybrid
Homo sapiens
23
CLEC7A
Two-hybrid
Homo sapiens
24
MAL
Two-hybrid
Homo sapiens
25
VSIG4
Affinity Capture-MS
Homo sapiens
26
GZMH
Affinity Capture-MS
Homo sapiens
27
AGR3
155465
Two-hybrid
Homo sapiens
28
TM2D3
Affinity Capture-MS
Homo sapiens
29
BRICD5
Affinity Capture-MS
Homo sapiens
30
SDF2L1
23753
Affinity Capture-MS
Homo sapiens
31
FBXO2
26232
Affinity Capture-MS
Homo sapiens
32
FOXK2
Affinity Capture-MS
Homo sapiens
33
TMEM190
Two-hybrid
Homo sapiens
34
TMEM109
79073
Two-hybrid
Homo sapiens
35
SMCO4
Two-hybrid
Homo sapiens
36
FAM46A
Two-hybrid
Homo sapiens
37
UBQLN4
56893
Two-hybrid
Homo sapiens
38
GXYLT1
283464
Affinity Capture-MS
Homo sapiens
39
CST11
Affinity Capture-MS
Homo sapiens
40
CLEC2B
Affinity Capture-MS
Homo sapiens
41
ST8SIA5
Affinity Capture-MS
Homo sapiens
42
ST14
6768
Affinity Capture-MS
Homo sapiens
43
HGFAC
3083
Affinity Capture-MS
Homo sapiens
44
LYPD4
Affinity Capture-MS
Homo sapiens
45
TMEM97
27346
Two-hybrid
Homo sapiens
46
UBXN6
80700
Co-fractionation
Homo sapiens
47
VAMP5
10791
Two-hybrid
Homo sapiens
View the network
image/svg+xml
Pathways in which SPINT1 is involved