Gene description for FOXK1
Gene name forkhead box K1
Gene symbol FOXK1
Other names/aliases FOXK1L
Species Homo sapiens
 Database cross references - FOXK1
ExoCarta ExoCarta_221937
Vesiclepedia VP_221937
Entrez Gene 221937
HGNC 23480
MIM 616302
UniProt P85037  
 FOXK1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for FOXK1
Molecular Function
    protein binding GO:0005515 IPI
    RNA polymerase II regulatory region sequence-specific DNA binding GO:0000977 IEA
    sequence-specific DNA binding RNA polymerase II transcription factor activity GO:0000981 IBA
Biological Process
    muscle organ development GO:0007517 IEA
    negative regulation of transcription, DNA-templated GO:0045892 IEA
    cell differentiation GO:0030154 IBA
    transcription from RNA polymerase II promoter GO:0006366 IEA
    anatomical structure morphogenesis GO:0009653 IBA
    negative regulation of cell growth GO:0030308 IEA
    regulation of transcription from RNA polymerase II promoter GO:0006357 IBA
    positive regulation of transcription, DNA-templated GO:0045893 IEA
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleolus GO:0005730 IDA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified FOXK1 in exosomes
1
Experiment ID 226
MISEV standards
EM
EV Biophysical techniques
GAPDH|CD9|FLOT1
EV Enriched markers
LMNA|H2AFX|ATP5A1|TOMM20
EV Negative markers
EV Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting
Authors Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 235
MISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Enriched markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 488
MISEV standards
EM
EV Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
CANX
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FOXK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 YWHAB 7529
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FOXK1 is involved
No pathways found





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