Gene description for FOXK1
Gene name forkhead box K1
Gene symbol FOXK1
Other names/aliases FOXK1L
Species Homo sapiens
 Database cross references - FOXK1
ExoCarta ExoCarta_221937
Vesiclepedia VP_221937
Entrez Gene 221937
HGNC 23480
MIM 616302
UniProt P85037  
 FOXK1 identified in exosomes derived from the following tissue/cell type
Endothelial cells 26027894    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for FOXK1
Molecular Function
    transcription cis-regulatory region binding GO:0000976 IDA
    RNA polymerase II cis-regulatory region sequence-specific DNA binding GO:0000978 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 IBA
    DNA-binding transcription factor activity, RNA polymerase II-specific GO:0000981 ISA
    DNA-binding transcription repressor activity, RNA polymerase II-specific GO:0001227 IDA
    DNA-binding transcription factor activity GO:0003700 IDA
    protein binding GO:0005515 IPI
    14-3-3 protein binding GO:0071889 IPI
    sequence-specific double-stranded DNA binding GO:1990837 IDA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IEA
    intracellular glucose homeostasis GO:0001678 IDA
    regulation of transcription by RNA polymerase II GO:0006357 IBA
    muscle organ development GO:0007517 IEA
    negative regulation of autophagy GO:0010507 IDA
    regulation of glucose metabolic process GO:0010906 IDA
    cell differentiation GO:0030154 IEA
    response to starvation GO:0042594 IDA
    negative regulation of DNA-templated transcription GO:0045892 IDA
    positive regulation of DNA-templated transcription GO:0045893 IDA
    canonical glycolysis GO:0061621 IDA
Subcellular Localization
    chromatin GO:0000785 ISA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 TAS
    cytoplasm GO:0005737 IDA
 Experiment description of studies that identified FOXK1 in exosomes
1
Experiment ID 226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘ Particle analysis
Identified molecule mRNA
Identification method Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID 26027894    
Organism Homo sapiens
Experiment description "Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors "Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name Journal of Extracellular Vesicles
Publication year 2015
Sample Endothelial cells
Sample name HMEC-1
Isolation/purification methods Differential ultracentrifugation
Sucrose density gradient
Flotation density 1.10 g/mL
Molecules identified in the study miRNA
Protein
snoRNA
lncRNA
yRNA
snRNA
mRNA
ncRNA
mtRNA
vtRNA
scaRNA
lincRNA
Methods used in the study Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
Western blotting
2
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
3
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FOXK1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KLF8  
Proximity Label-MS Homo sapiens
2 HDAC2 3066
Affinity Capture-MS Homo sapiens
3 NPRL2  
Affinity Capture-MS Homo sapiens
4 ELF1 1997
Proximity Label-MS Homo sapiens
5 COIL  
Proximity Label-MS Homo sapiens
6 FEV  
Proximity Label-MS Homo sapiens
7 RB1 5925
Affinity Capture-MS Homo sapiens
8 TBC1D4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 RABEP2 79874
Affinity Capture-MS Homo sapiens
10 PLBD1 79887
Affinity Capture-MS Homo sapiens
11 TGIF2LX  
Affinity Capture-MS Homo sapiens
12 BTF3 689
Affinity Capture-MS Homo sapiens
13 NFATC1 4772
Affinity Capture-MS Homo sapiens
14 TLX3  
Proximity Label-MS Homo sapiens
15 SOX2  
Proximity Label-MS Homo sapiens
16 USP24 23358
Co-fractionation Homo sapiens
17 CHEK2  
Affinity Capture-MS Homo sapiens
18 DVL3 1857
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 AGGF1  
Affinity Capture-MS Homo sapiens
20 RABGGTB 5876
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 ASXL1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
22 ANKRD28 23243
Affinity Capture-MS Homo sapiens
23 ELF4 2000
Proximity Label-MS Homo sapiens
24 SCRIB 23513
Affinity Capture-MS Homo sapiens
25 CENPA  
Proximity Label-MS Homo sapiens
26 APEX1 328
Proximity Label-MS Homo sapiens
27 TEX264 51368
Affinity Capture-MS Homo sapiens
28 NCOR1  
Affinity Capture-MS Homo sapiens
29 RNPS1 10921
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
30 PFKL 5211
Co-fractionation Homo sapiens
31 PDGFRB 5159
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 UBE2O 63893
Affinity Capture-MS Homo sapiens
33 TBL1XR1 79718
Affinity Capture-MS Homo sapiens
34 RFXANK  
Affinity Capture-MS Homo sapiens
35 METTL22  
Affinity Capture-MS Homo sapiens
36 NUP35 129401
Proximity Label-MS Homo sapiens
37 NUDT3 11165
Co-fractionation Homo sapiens
38 ARID4B  
Affinity Capture-MS Homo sapiens
39 VASP 7408
Co-fractionation Homo sapiens
40 Fam60a 56306
Affinity Capture-MS Mus musculus
41 PPP6R1 22870
Affinity Capture-MS Homo sapiens
42 YWHAG 7532
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
43 FAM129A 116496
Co-fractionation Homo sapiens
44 AGTPBP1  
Affinity Capture-MS Homo sapiens
45 TULP3 7289
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 TMEM192 201931
Affinity Capture-MS Homo sapiens
47 YWHAH 7533
Affinity Capture-MS Homo sapiens
48 ASH2L 9070
Proximity Label-MS Homo sapiens
49 LHX4  
Proximity Label-MS Homo sapiens
50 MAPK14 1432
Affinity Capture-MS Homo sapiens
51 MYCN  
Affinity Capture-MS Homo sapiens
52 NPAS1  
Affinity Capture-MS Homo sapiens
53 KLF15  
Proximity Label-MS Homo sapiens
54 SOX5 6660
Proximity Label-MS Homo sapiens
55 AMOT  
Affinity Capture-MS Homo sapiens
56 DVL2 1856
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 TP53BP1 7158
Affinity Capture-MS Homo sapiens
58 DYRK1B  
Affinity Capture-MS Homo sapiens
59 YWHAB 7529
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
60 GARS 2617
Co-fractionation Homo sapiens
61 BAG5 9529
Affinity Capture-MS Homo sapiens
62 UBR4 23352
Affinity Capture-MS Homo sapiens
63 EIF2AK3  
Affinity Capture-MS Homo sapiens
64 KLF9 687
Proximity Label-MS Homo sapiens
65 RBBP7 5931
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
66 PHKA2 5256
Affinity Capture-MS Homo sapiens
67 UBE4B 10277
Co-fractionation Homo sapiens
68 HIST1H4A 8359
Affinity Capture-MS Homo sapiens
69 MCC 4163
Affinity Capture-MS Homo sapiens
70 FHL3 2275
Affinity Capture-MS Homo sapiens
71 TP53 7157
Affinity Capture-MS Homo sapiens
72 IRF2 3660
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
73 CTBP1 1487
Affinity Capture-MS Homo sapiens
74 BRMS1 25855
Affinity Capture-MS Homo sapiens
75 TBR1  
Proximity Label-MS Homo sapiens
76 ELK3  
Proximity Label-MS Homo sapiens
77 CCT5 22948
Co-fractionation Homo sapiens
78 GOLGA2 2801
Proximity Label-MS Homo sapiens
79 USP47 55031
Affinity Capture-MS Homo sapiens
80 PABPC1 26986
Co-fractionation Homo sapiens
81 SREBF2 6721
Positive Genetic Homo sapiens
82 LHX2  
Proximity Label-MS Homo sapiens
83 NTRK1 4914
Affinity Capture-MS Homo sapiens
84 FOXK2  
Affinity Capture-MS Homo sapiens
85 DCAF7 10238
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 LHX3  
Proximity Label-MS Homo sapiens
87 FBLN5 10516
Affinity Capture-MS Homo sapiens
88 SUDS3  
Affinity Capture-MS Homo sapiens
89 RAB11FIP5 26056
Co-fractionation Homo sapiens
90 HCFC1 3054
Affinity Capture-Western Homo sapiens
91 TANC2  
Affinity Capture-MS Homo sapiens
92 MYCL  
Affinity Capture-MS Homo sapiens
93 KLF12  
Proximity Label-MS Homo sapiens
94 CEBPA  
Proximity Label-MS Homo sapiens
Protein-peptide Homo sapiens
95 USP28 57646
Affinity Capture-MS Homo sapiens
96 ARID4A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
97 ING2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
98 RBL1  
Affinity Capture-MS Homo sapiens
99 PSG11  
Affinity Capture-MS Homo sapiens
100 ZBED6CL  
Affinity Capture-MS Homo sapiens
101 PAX6  
Proximity Label-MS Homo sapiens
102 ASXL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
103 GAPVD1 26130
Co-fractionation Homo sapiens
104 AHCY 191
Affinity Capture-MS Homo sapiens
105 ASXL3  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
106 AHCYL1 10768
Affinity Capture-MS Homo sapiens
107 XPO1 7514
Affinity Capture-MS Homo sapiens
108 BAG2 9532
Affinity Capture-MS Homo sapiens
109 FAM60A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
110 CCT6A 908
Co-fractionation Homo sapiens
111 EEF2 1938
Co-fractionation Homo sapiens
112 KCMF1 56888
Affinity Capture-MS Homo sapiens
113 MEX3A  
Affinity Capture-RNA Homo sapiens
114 CD6 923
Affinity Capture-MS Homo sapiens
115 TNFRSF1A 7132
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 TFAP4 7023
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
117 PHKG2 5261
Affinity Capture-MS Homo sapiens
118 BAP1 8314
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-purification Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
119 IKZF1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
120 BRMS1L  
Affinity Capture-MS Homo sapiens
121 CCDC85A  
Affinity Capture-MS Homo sapiens
122 SAP30 8819
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
123 PRMT5 10419
Co-fractionation Homo sapiens
124 RPA3 6119
Proximity Label-MS Homo sapiens
125 TPM1 7168
Co-fractionation Homo sapiens
126 UBA2 10054
Co-fractionation Homo sapiens
127 DNAI2  
Affinity Capture-MS Homo sapiens
128 ZBTB39  
Affinity Capture-MS Homo sapiens
129 UHRF1BP1 54887
Affinity Capture-MS Homo sapiens
130 RBL2 5934
Affinity Capture-MS Homo sapiens
131 TP63  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 CLASRP  
Affinity Capture-MS Homo sapiens
133 CMYA5 202333
Co-fractionation Homo sapiens
134 KIAA0513 9764
Affinity Capture-MS Homo sapiens
135 FAXC  
Affinity Capture-MS Homo sapiens
136 LMNA 4000
Co-fractionation Homo sapiens
137 NFATC2  
Affinity Capture-MS Homo sapiens
138 FHL2 2274
Affinity Capture-MS Homo sapiens
139 RIPPLY3  
Affinity Capture-MS Homo sapiens
140 NAA10 8260
Co-fractionation Homo sapiens
141 METTL21B  
Affinity Capture-MS Homo sapiens
142 AR 367
Proximity Label-MS Homo sapiens
143 TLX1  
Proximity Label-MS Homo sapiens
144 TRAK2 66008
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
145 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
146 ZNF428  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
147 FOXO3  
Affinity Capture-MS Homo sapiens
148 Bap1  
Affinity Capture-MS Mus musculus
149 Max  
Affinity Capture-MS Mus musculus
150 SAP130  
Affinity Capture-MS Homo sapiens
151 MAX  
Affinity Capture-MS Homo sapiens
152 SIN3A  
Affinity Capture-MS Homo sapiens
153 SOX15  
Proximity Label-MS Homo sapiens
154 ABHD10 55347
Affinity Capture-MS Homo sapiens
155 KLHDC3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
156 MEN1 4221
Affinity Capture-MS Homo sapiens
157 KLF3  
Proximity Label-MS Homo sapiens
158 SPINT1 6692
Affinity Capture-MS Homo sapiens
159 DOPEY2 9980
Affinity Capture-MS Homo sapiens
160 BID  
Co-fractionation Homo sapiens
161 FYCO1 79443
Affinity Capture-MS Homo sapiens
162 HDAC1 3065
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
163 PAX9  
Proximity Label-MS Homo sapiens
164 DDX58 23586
Affinity Capture-RNA Homo sapiens
165 PSMA1 5682
Co-fractionation Homo sapiens
166 PPM1G 5496
Affinity Capture-MS Homo sapiens
167 KLF5  
Proximity Label-MS Homo sapiens
168 GSTM3 2947
Affinity Capture-MS Homo sapiens
169 AHCYL2 23382
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
170 ANKRD52 283373
Affinity Capture-MS Homo sapiens
171 HSPBP1 23640
Affinity Capture-MS Homo sapiens
172 ING1  
Affinity Capture-MS Homo sapiens
173 EEF1D 1936
Co-fractionation Homo sapiens
174 PYGB 5834
Co-fractionation Homo sapiens
175 UBA7 7318
Affinity Capture-MS Homo sapiens
176 ATP1A1 476
Affinity Capture-MS Homo sapiens
177 KANK2 25959
Affinity Capture-MS Homo sapiens
178 SERBP1 26135
Affinity Capture-MS Homo sapiens
179 ELF2  
Proximity Label-MS Homo sapiens
180 UBXN6 80700
Affinity Capture-MS Homo sapiens
181 GATAD1  
Affinity Capture-MS Homo sapiens
182 VIM 7431
Co-fractionation Homo sapiens
183 AP5Z1 9907
Affinity Capture-MS Homo sapiens
184 SP7  
Proximity Label-MS Homo sapiens
185 GPS2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
186 POLD2 5425
Co-fractionation Homo sapiens
187 EP300 2033
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
188 NHLH1  
Proximity Label-MS Homo sapiens
View the network image/svg+xml
 Pathways in which FOXK1 is involved
PathwayEvidenceSource
Deubiquitination TAS Reactome
Metabolism of proteins TAS Reactome
Post-translational protein modification TAS Reactome
UCH proteinases TAS Reactome





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here