Gene description for FBLN5
Gene name fibulin 5
Gene symbol FBLN5
Other names/aliases ADCL2
ARCL1A
ARMD3
DANCE
EVEC
FIBL-5
UP50
Species Homo sapiens
 Database cross references - FBLN5
ExoCarta ExoCarta_10516
Vesiclepedia VP_10516
Entrez Gene 10516
HGNC 3602
MIM 604580
UniProt Q9UBX5  
 FBLN5 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for FBLN5
Molecular Function
    integrin binding GO:0005178 ISS
    calcium ion binding GO:0005509 IEA
    protein binding GO:0005515 IPI
    extracellular matrix constituent conferring elasticity GO:0030023 RCA
    protein homodimerization activity GO:0042803 IDA
Biological Process
    cell-matrix adhesion GO:0007160 TAS
    protein localization to cell surface GO:0034394 ISS
    secretion GO:0046903 IDA
    elastic fiber assembly GO:0048251 IBA
    elastic fiber assembly GO:0048251 IMP
    elastic fiber assembly GO:0048251 ISS
    regulation of removal of superoxide radicals GO:2000121 ISS
Subcellular Localization
    extracellular region GO:0005576 HDA
    extracellular region GO:0005576 TAS
    extracellular space GO:0005615 HDA
    extracellular matrix GO:0031012 TAS
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 HDA
    collagen-containing extracellular matrix GO:0062023 IDA
    extracellular exosome GO:0070062 HDA
    elastic fiber GO:0071953 ISS
 Experiment description of studies that identified FBLN5 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Osteoarthritic cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 496
MISEV standards
✘ Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name Healthy cartilage
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for FBLN5
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ZNF407  
Affinity Capture-MS Homo sapiens
2 ZNF282  
Affinity Capture-MS Homo sapiens
3 FOXF1 2294
Affinity Capture-MS Homo sapiens
4 ARHGAP21 57584
Affinity Capture-MS Homo sapiens
5 ZNF440  
Affinity Capture-MS Homo sapiens
6 UTRN 7402
Affinity Capture-MS Homo sapiens
7 MAGEA3  
Affinity Capture-MS Homo sapiens
8 PAXIP1  
Two-hybrid Homo sapiens
9 ZBTB34 403341
Affinity Capture-MS Homo sapiens
10 EMILIN2 84034
Affinity Capture-MS Homo sapiens
11 ATF7  
Affinity Capture-MS Homo sapiens
12 FOXF2  
Affinity Capture-MS Homo sapiens
13 DISC1 27185
Affinity Capture-MS Homo sapiens
14 RPL10 6134
Affinity Capture-MS Homo sapiens
15 ZNF320  
Affinity Capture-MS Homo sapiens
16 POM121  
Affinity Capture-MS Homo sapiens
17 ZNF92  
Affinity Capture-MS Homo sapiens
18 ATN1  
Two-hybrid Homo sapiens
19 ZNF45  
Affinity Capture-MS Homo sapiens
20 PCGF2 7703
Affinity Capture-MS Homo sapiens
21 ZBTB33  
Affinity Capture-MS Homo sapiens
22 SULF2 55959
Affinity Capture-MS Homo sapiens
23 ZNF408  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 DYRK1A 1859
Affinity Capture-MS Homo sapiens
25 TGFB1 7040
Affinity Capture-MS Homo sapiens
26 ZNF74  
Affinity Capture-MS Homo sapiens
27 ATXN2L 11273
Affinity Capture-MS Homo sapiens
28 SENP1  
Affinity Capture-MS Homo sapiens
29 POLR2I 5438
Affinity Capture-MS Homo sapiens
30 PHC3  
Affinity Capture-MS Homo sapiens
31 ZNF518A 9849
Affinity Capture-MS Homo sapiens
32 DUSP11  
Affinity Capture-MS Homo sapiens
33 ZNF460  
Affinity Capture-MS Homo sapiens
34 FBXW8 26259
Affinity Capture-MS Homo sapiens
35 RECQL4  
Affinity Capture-MS Homo sapiens
36 ST14 6768
Affinity Capture-MS Homo sapiens
37 OBSL1 23363
Affinity Capture-MS Homo sapiens
38 ZNF737  
Affinity Capture-MS Homo sapiens
39 POLRMT 5442
Affinity Capture-MS Homo sapiens
40 SMOC1 64093
Affinity Capture-MS Homo sapiens
41 ZXDB  
Affinity Capture-MS Homo sapiens
42 PEAK1 79834
Affinity Capture-MS Homo sapiens
43 IGFL3  
Affinity Capture-MS Homo sapiens
44 SETD5 55209
Affinity Capture-MS Homo sapiens
45 CEP57  
Affinity Capture-MS Homo sapiens
46 GTSE1 51512
Affinity Capture-MS Homo sapiens
47 TTLL5  
Affinity Capture-MS Homo sapiens
48 ZNF273  
Affinity Capture-MS Homo sapiens
49 ZNHIT6 54680
Affinity Capture-MS Homo sapiens
50 FAM120A 23196
Affinity Capture-MS Homo sapiens
51 GFI1B  
Two-hybrid Homo sapiens
52 HOXB3 3213
Affinity Capture-MS Homo sapiens
53 PRNP 5621
Two-hybrid Homo sapiens
54 POLR1A 25885
Affinity Capture-MS Homo sapiens
55 ZNF550  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 ZIC2  
Affinity Capture-MS Homo sapiens
57 ZBTB40  
Affinity Capture-MS Homo sapiens
58 HRG 3273
Affinity Capture-MS Homo sapiens
59 ZNF444  
Affinity Capture-MS Homo sapiens
60 TOP3B 8940
Two-hybrid Homo sapiens
61 FBLN1 2192
Affinity Capture-MS Homo sapiens
62 KLHDC2  
Affinity Capture-MS Homo sapiens
63 PAX3  
Affinity Capture-MS Homo sapiens
64 SUPT7L  
Affinity Capture-MS Homo sapiens
65 KRBA1  
Affinity Capture-MS Homo sapiens
66 TKTL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
67 TAF9B  
Affinity Capture-MS Homo sapiens
68 KIAA1683  
Affinity Capture-MS Homo sapiens
69 ZFP41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 GDF11 10220
Affinity Capture-MS Homo sapiens
71 EMILIN3  
Affinity Capture-MS Homo sapiens
72 ZNF324  
Affinity Capture-MS Homo sapiens
73 CHEK1  
Affinity Capture-MS Homo sapiens
74 MEOX2  
Two-hybrid Homo sapiens
75 TRMT2B  
Affinity Capture-MS Homo sapiens
76 EED  
Affinity Capture-MS Homo sapiens
77 SLX4IP  
Affinity Capture-MS Homo sapiens
78 TAF6L 10629
Affinity Capture-MS Homo sapiens
79 ZNF8  
Affinity Capture-MS Homo sapiens
80 ZBTB44  
Affinity Capture-MS Homo sapiens
81 ZNF747  
Affinity Capture-MS Homo sapiens
82 TRIP13 9319
Two-hybrid Homo sapiens
83 CBX4  
Affinity Capture-MS Homo sapiens
84 RECQL5  
Affinity Capture-MS Homo sapiens
85 KIF1B 23095
Affinity Capture-MS Homo sapiens
86 FAM76B  
Affinity Capture-MS Homo sapiens
87 IQSEC1 9922
Affinity Capture-MS Homo sapiens
88 MAP7 9053
Affinity Capture-MS Homo sapiens
89 R3HDM1  
Affinity Capture-MS Homo sapiens
90 ZNF77  
Affinity Capture-MS Homo sapiens
91 FOXG1  
Affinity Capture-MS Homo sapiens
92 LTBP4 8425
Affinity Capture-MS Homo sapiens
93 EGFL7 51162
Affinity Capture-MS Homo sapiens
94 TRIM68 55128
Affinity Capture-MS Homo sapiens
95 POLR2A 5430
Affinity Capture-MS Homo sapiens
96 OTX1  
Two-hybrid Homo sapiens
97 ZNF492  
Affinity Capture-MS Homo sapiens
98 ARID1B 57492
Affinity Capture-MS Homo sapiens
99 JUN 3725
Affinity Capture-MS Homo sapiens
100 POLR1E  
Affinity Capture-MS Homo sapiens
101 ASPH 444
Affinity Capture-MS Homo sapiens
102 DNAJC10 54431
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
103 ZNF724P  
Affinity Capture-MS Homo sapiens
104 LTBP1 4052
Affinity Capture-MS Homo sapiens
105 CHD8 57680
Affinity Capture-MS Homo sapiens
106 BMI1  
Affinity Capture-MS Homo sapiens
107 PATZ1  
Affinity Capture-MS Homo sapiens
108 SENP5  
Affinity Capture-MS Homo sapiens
109 ZNF136  
Affinity Capture-MS Homo sapiens
110 ZNF696  
Affinity Capture-MS Homo sapiens
111 FOXK1 221937
Affinity Capture-MS Homo sapiens
112 YRDC 79693
Affinity Capture-MS Homo sapiens
113 HOXC9  
Affinity Capture-MS Homo sapiens
114 CCDC8  
Affinity Capture-MS Homo sapiens
115 PRR14L 253143
Affinity Capture-MS Homo sapiens
116 ZNF765  
Affinity Capture-MS Homo sapiens
117 ZNF669  
Affinity Capture-MS Homo sapiens
118 ALG13 79868
Affinity Capture-MS Homo sapiens
119 LOXL1 4016
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
120 METTL17  
Affinity Capture-MS Homo sapiens
121 ZNF235  
Affinity Capture-MS Homo sapiens
122 ZNF430  
Affinity Capture-MS Homo sapiens
123 RAD54L2  
Affinity Capture-MS Homo sapiens
124 ZNF746  
Affinity Capture-MS Homo sapiens
125 KLHDC3  
Affinity Capture-MS Homo sapiens
126 NUFIP2 57532
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
127 DGCR8  
Affinity Capture-MS Homo sapiens
128 TRMT61B  
Affinity Capture-MS Homo sapiens
129 SECISBP2L  
Affinity Capture-MS Homo sapiens
130 TROAP  
Affinity Capture-MS Homo sapiens
131 ZNF764 92595
Affinity Capture-MS Homo sapiens
132 ZNF253  
Affinity Capture-MS Homo sapiens
133 DDX58 23586
Affinity Capture-RNA Homo sapiens
134 PRRC2B  
Affinity Capture-MS Homo sapiens
135 CREB5 9586
Affinity Capture-MS Homo sapiens
136 AGRN 375790
Affinity Capture-MS Homo sapiens
137 ZNF627  
Affinity Capture-MS Homo sapiens
138 APPBP2  
Affinity Capture-MS Homo sapiens
139 ZNF195  
Affinity Capture-MS Homo sapiens
140 ZNF620  
Affinity Capture-MS Homo sapiens
141 HIC2 23119
Affinity Capture-MS Homo sapiens
142 KIF18B 146909
Affinity Capture-MS Homo sapiens
143 NTN5  
Affinity Capture-MS Homo sapiens
144 ZBTB9  
Affinity Capture-MS Homo sapiens
145 RBPMS2  
Affinity Capture-MS Homo sapiens
146 RNF111 54778
Affinity Capture-MS Homo sapiens
147 LAMA5 3911
Affinity Capture-MS Homo sapiens
148 CUL7 9820
Affinity Capture-MS Homo sapiens
149 PHC2  
Affinity Capture-MS Homo sapiens
150 ZNF331  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
151 ZBED4 9889
Affinity Capture-MS Homo sapiens
152 CILP2  
Affinity Capture-MS Homo sapiens
153 ZNF865  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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