Gene description for POLR2I
Gene name polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
Gene symbol POLR2I
Other names/aliases RPB9
hRPB14.5
Species Homo sapiens
 Database cross references - POLR2I
ExoCarta ExoCarta_5438
Entrez Gene 5438
HGNC 9196
MIM 180662
UniProt P36954  
 POLR2I identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Hepatocytes 26054723    
 Gene ontology annotations for POLR2I
Molecular Function
    DNA-directed RNA polymerase activity GO:0003899 IEA
    zinc ion binding GO:0008270 IEA
    DNA binding GO:0003677 IEA
Biological Process
    viral process GO:0016032 TAS
    gene expression GO:0010467 TAS
    somatic stem cell maintenance GO:0035019 TAS
    nucleotide-excision repair GO:0006289 TAS
    transcription from RNA polymerase II promoter GO:0006366 TAS
    RNA splicing GO:0008380 TAS
    positive regulation of viral transcription GO:0050434 TAS
    piRNA metabolic process GO:0034587 TAS
    DNA repair GO:0006281 TAS
    7-methylguanosine mRNA capping GO:0006370 TAS
    maintenance of transcriptional fidelity during DNA-templated transcription elongation from RNA polymerase II promoter GO:0001193 IBA
    transcription-coupled nucleotide-excision repair GO:0006283 TAS
    transcription initiation from RNA polymerase II promoter GO:0006367 TAS
    mRNA splicing, via spliceosome GO:0000398 TAS
    transcription elongation from RNA polymerase II promoter GO:0006368 TAS
Subcellular Localization
    nucleus GO:0005634 IDA
    DNA-directed RNA polymerase II, core complex GO:0005665 IDA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified POLR2I in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 237
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
 Protein-protein interactions for POLR2I
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 CRSP7  
Affinity Capture-MS Homo sapiens
2 MED19  
Affinity Capture-MS Homo sapiens
3 IXL  
Affinity Capture-MS Homo sapiens
4 TRG20  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which POLR2I is involved
PathwayEvidenceSource
Abortive elongation of HIV-1 transcript in the absence of Tat TAS Reactome
Dual incision reaction in TC-NER TAS Reactome
Formation of HIV elongation complex in the absence of HIV Tat IEA Reactome
Formation of HIV-1 elongation complex containing HIV-1 Tat IEA Reactome
Formation of RNA Pol II elongation complex TAS Reactome
Formation of the Early Elongation Complex TAS Reactome
Formation of the HIV-1 Early Elongation Complex IEA Reactome
Formation of transcription-coupled NER (TC-NER) repair complex TAS Reactome
HIV elongation arrest and recovery IEA Reactome
HIV Transcription Initiation IEA Reactome
MicroRNA (miRNA) biogenesis TAS Reactome
mRNA Capping TAS Reactome
mRNA Splicing - Major Pathway TAS Reactome
mRNA Splicing - Minor Pathway TAS Reactome
Pausing and recovery of HIV elongation TAS Reactome
Pausing and recovery of Tat-mediated HIV elongation TAS Reactome
PIWI-interacting RNA (piRNA) biogenesis TAS Reactome
POU5F1 (OCT4), SOX2, NANOG activate genes related to proliferation TAS Reactome
Processing of Capped Intron-Containing Pre-mRNA TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE TAS Reactome
RNA Pol II CTD phosphorylation and interaction with CE IEA Reactome
RNA Polymerase II HIV Promoter Escape IEA Reactome
RNA Polymerase II Pre-transcription Events TAS Reactome
RNA Polymerase II Promoter Escape TAS Reactome
RNA Polymerase II Transcription Elongation TAS Reactome
RNA Polymerase II Transcription Initiation TAS Reactome
RNA Polymerase II Transcription Initiation And Promoter Clearance TAS Reactome
RNA Polymerase II Transcription Pre-Initiation And Promoter Opening TAS Reactome
Tat-mediated elongation of the HIV-1 transcript TAS Reactome
Tat-mediated HIV elongation arrest and recovery IEA Reactome
Transcription of the HIV genome IEA Reactome
Transcription-coupled NER (TC-NER) TAS Reactome
Transcriptional regulation by small RNAs TAS Reactome
Transcriptional regulation of pluripotent stem cells TAS Reactome
Viral Messenger RNA Synthesis TAS Reactome





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