Gene description for SRC
Gene name SRC proto-oncogene, non-receptor tyrosine kinase
Gene symbol SRC
Other names/aliases ASV
SRC1
c-SRC
p60-Src
Species Homo sapiens
 Database cross references - SRC
ExoCarta ExoCarta_6714
Entrez Gene 6714
HGNC 11283
MIM 190090
UniProt P12931  
 SRC identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
B cells 20458337    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Colorectal cancer cells 25890246    
Melanoma cells 25950383    
Melanoma cells 25950383    
Neuroblastoma cells 25944692    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Squamous carcinoma cells 20124223    
Urine 19056867    
Urine 22418980    
Urine 22418980    
 Gene ontology annotations for SRC
Molecular Function
    integrin binding GO:0005178 TAS
    enzyme binding GO:0019899 IPI
    kinase activity GO:0016301 TAS
    SH3/SH2 adaptor activity GO:0005070 TAS
    phosphoprotein binding GO:0051219 IPI
    hormone receptor binding GO:0051427 IBA
    growth factor receptor binding GO:0070851 IPI
    protein binding GO:0005515 IPI
    SH2 domain binding GO:0042169 IPI
    scaffold protein binding GO:0097110 IPI
    heme binding GO:0020037 IDA
    non-membrane spanning protein tyrosine kinase activity GO:0004715 TAS
    protein kinase activity GO:0004672 TAS
    protein tyrosine kinase activity GO:0004713 TAS
    insulin receptor binding GO:0005158 IEA
    ephrin receptor binding GO:0046875 IPI
    estrogen receptor binding GO:0030331 IEA
    ATP binding GO:0005524 IEA
    receptor binding GO:0005102 IPI
    ion channel binding GO:0044325 IPI
    protein kinase C binding GO:0005080 IEA
    protein C-terminus binding GO:0008022 IEA
Biological Process
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IMP
    bone resorption GO:0045453 ISS
    axon guidance GO:0007411 TAS
    signal transduction GO:0007165 TAS
    regulation of protein binding GO:0043393 IEA
    stimulatory C-type lectin receptor signaling pathway GO:0002223 TAS
    regulation of podosome assembly GO:0071801 IBA
    negative regulation of protein homooligomerization GO:0032463 IMP
    regulation of cell cycle GO:0051726 IBA
    positive regulation of epithelial cell migration GO:0010634 IMP
    Ras protein signal transduction GO:0007265 TAS
    uterus development GO:0060065 IEA
    response to mechanical stimulus GO:0009612 IEA
    negative regulation of focal adhesion assembly GO:0051895 ISS
    oogenesis GO:0048477 IEA
    peptidyl-tyrosine autophosphorylation GO:0038083 IBA
    intracellular signal transduction GO:0035556 IDA
    response to interleukin-1 GO:0070555 IMP
    cellular response to hypoxia GO:0071456 IEA
    branching involved in mammary gland duct morphogenesis GO:0060444 IEA
    positive regulation of glucose metabolic process GO:0010907 IEA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    peptidyl-serine phosphorylation GO:0018105 IEA
    regulation of caveolin-mediated endocytosis GO:2001286 IMP
    regulation of cell projection assembly GO:0060491 IEA
    cell proliferation GO:0008283 IEA
    blood coagulation GO:0007596 TAS
    positive regulation of lamellipodium morphogenesis GO:2000394 IMP
    positive regulation of cytokine secretion GO:0050715 IEA
    progesterone receptor signaling pathway GO:0050847 ISS
    fibroblast growth factor receptor signaling pathway GO:0008543 TAS
    regulation of cell-cell adhesion GO:0022407 IMP
    positive regulation of MAP kinase activity GO:0043406 IEA
    negative regulation of intrinsic apoptotic signaling pathway GO:2001243 IMP
    cellular response to insulin stimulus GO:0032869 IEA
    cellular response to peptide hormone stimulus GO:0071375 ISS
    positive regulation of apoptotic process GO:0043065 IEA
    forebrain development GO:0030900 IEA
    angiotensin-activated signaling pathway involved in heart process GO:0086098 ISS
    transforming growth factor beta receptor signaling pathway GO:0007179 IMP
    positive regulation of ERK1 and ERK2 cascade GO:0070374 IEA
    cell adhesion GO:0007155 IBA
    positive regulation of smooth muscle cell migration GO:0014911 IEA
    positive regulation of peptidyl-tyrosine phosphorylation GO:0050731 IC
    cellular response to progesterone stimulus GO:0071393 ISS
    positive regulation of transcription, DNA-templated GO:0045893 IEA
    stress fiber assembly GO:0043149 IMP
    response to nutrient levels GO:0031667 IEA
    cellular response to platelet-derived growth factor stimulus GO:0036120 IEA
    peptidyl-tyrosine phosphorylation GO:0018108 IDA
    Fc-gamma receptor signaling pathway involved in phagocytosis GO:0038096 TAS
    leukocyte migration GO:0050900 TAS
    response to drug GO:0042493 IEA
    negative regulation of transcription, DNA-templated GO:0045892 IEA
    positive regulation of platelet-derived growth factor receptor signaling pathway GO:0010641 IEA
    signal complex assembly GO:0007172 TAS
    positive regulation of protein autophosphorylation GO:0031954 IEA
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IMP
    small GTPase mediated signal transduction GO:0007264 TAS
    response to virus GO:0009615 IEA
    membrane organization GO:0061024 TAS
    regulation of early endosome to late endosome transport GO:2000641 IMP
    response to acidic pH GO:0010447 IEA
    cell cycle GO:0007049 IEA
    regulation of vascular permeability GO:0043114 TAS
    regulation of intracellular estrogen receptor signaling pathway GO:0033146 IEA
    positive regulation of integrin activation GO:0033625 TAS
    platelet activation GO:0030168 TAS
    positive regulation of podosome assembly GO:0071803 IEA
    positive regulation of insulin receptor signaling pathway GO:0046628 IEA
    positive regulation of cyclin-dependent protein serine/threonine kinase activity GO:0045737 IEA
    response to hydrogen peroxide GO:0042542 IEA
    viral process GO:0016032 IEA
    protein autophosphorylation GO:0046777 IDA
    positive regulation of phosphatidylinositol 3-kinase activity GO:0043552 IEA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IEA
    ephrin receptor signaling pathway GO:0048013 TAS
    response to mineralocorticoid GO:0051385 IEA
    positive regulation of DNA biosynthetic process GO:2000573 IEA
    osteoclast development GO:0036035 IBA
    negative regulation of apoptotic process GO:0043066 IMP
    T cell costimulation GO:0031295 TAS
    cell differentiation GO:0030154 IBA
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    innate immune response GO:0045087 TAS
    adherens junction organization GO:0034332 IEA
    regulation of epithelial cell migration GO:0010632 IMP
    integrin-mediated signaling pathway GO:0007229 IMP
    negative regulation of anoikis GO:2000811 IMP
    negative regulation of mitochondrial depolarization GO:0051902 IMP
    transcytosis GO:0045056 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    cellular response to fatty acid GO:0071398 IEA
    cellular response to lipopolysaccharide GO:0071222 IEA
    positive regulation of protein kinase B signaling GO:0051897 IMP
    substrate adhesion-dependent cell spreading GO:0034446 IEA
    response to electrical stimulus GO:0051602 IEA
    activation of protein kinase B activity GO:0032148 IEA
    single organismal cell-cell adhesion GO:0016337 IEA
    regulation of bone resorption GO:0045124 TAS
    regulation of cell proliferation GO:0042127 IBA
Subcellular Localization
    perinuclear region of cytoplasm GO:0048471 IEA
    nucleus GO:0005634 IEA
    extrinsic component of cytoplasmic side of plasma membrane GO:0031234 IBA
    mitochondrial inner membrane GO:0005743 IDA
    actin filament GO:0005884 IEA
    lysosome GO:0005764 IDA
    neuron projection GO:0043005 IEA
    ruffle membrane GO:0032587 IEA
    extracellular exosome GO:0070062 IDA
    mitochondrion GO:0005739 IDA
    plasma membrane GO:0005886 TAS
    late endosome GO:0005770 IDA
    postsynaptic density GO:0014069 IEA
    cytoplasm GO:0005737 IDA
    caveola GO:0005901 IDA
    cytosol GO:0005829 TAS
 Experiment description of studies that identified SRC in exosomes
1
Experiment ID 79
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 81
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
4
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 282
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
8
Experiment ID 283
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Ultracentrifugation - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
9
Experiment ID 286
ISEV standards
✔
CEM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81|EpCAM
EV Membrane markers
✘ EV Negative markers
✔
DLS
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25890246    
Organism Homo sapiens
Experiment description Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct.
Authors Xu R, Greening DW, Rai A, Ji H, Simpson RJ.
Journal name Methods
Publication year 2015
Sample Colorectal cancer cells
Sample name LIM1863 - Sequential centrifugal ultrafiltration - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Sequential centrifugal ultrafiltration
Centrifugal concentration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
10
Experiment ID 259
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name A375M
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
11
Experiment ID 260
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
FLOT1|CD81
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25950383    
Organism Homo sapiens
Experiment description Proteome characterization of melanoma exosomes reveals a specific signature for metastatic cell lines
Authors Lazar I, Clement E, Ducoux-Petit M, Denat L, Soldan V, Dauvillier S, Balor S4, Burlet-Schiltz O1, Larue L, Muller C Nieto L
Journal name Pigment Cell Melanoma Res
Publication year 2015
Sample Melanoma cells
Sample name 1205Lu
Isolation/purification methods Differential centrifugation
Unltracentrifugation
Sucrose density gradient
Flotation density 1.13 - 1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 224
ISEV standards
✔
EM|AFM
EV Biophysical techniques
✔
Alix|TSG101
EV Cytosolic markers
✔
CD63|CD81
EV Membrane markers
✔
GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25944692    
Organism Homo sapiens
Experiment description Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S
Journal name Oncotarget
Publication year 2015
Sample Neuroblastoma cells
Sample name SH-SY5Y
Isolation/purification methods Differential centrifugation
Ultracentrifugation
OptiPrep density gradient
Flotation density 1.10 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Western blotting
13
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
14
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
15
Experiment ID 231
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD63|CD9
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
16
Experiment ID 232
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
17
Experiment ID 233
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✘ EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
18
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
19
Experiment ID 63
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
AQP2
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
20
Experiment ID 196
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal high density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
21
Experiment ID 197
ISEV standards
✔
EM
EV Biophysical techniques
✔
Alix|TSG101|HSP70
EV Cytosolic markers
✔
CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 22418980    
Organism Homo sapiens
Experiment description A multiplex quantitative proteomics strategy for protein biomarker studies in urinary exosomes.
Authors Raj DA, Fiume I, Capasso G, Pocsfalvi G.
Journal name Kidney Int
Publication year 2012
Sample Urine
Sample name Urine - Normal low density
Isolation/purification methods Differential centrifugation
Sucrose cushion
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for SRC
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ADRBK1 156
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
2 ND2  
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
3 AXL 558
Affinity Capture-MS Homo sapiens
Far Western Homo sapiens
4 NCOA6  
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
5 ARHGAP1 392
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
6 SHC1 6464
Affinity Capture-MS Homo sapiens
7 MATK  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
8 PXN  
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
9 CSK 1445
Invivo Homo sapiens
10 THRB 7068
Reconstituted Complex Homo sapiens
11 STAT5A 6776
Affinity Capture-MS Homo sapiens
12 GJA1 2697
Affinity Capture-MS Homo sapiens
13 SNURF  
Phenotypic Enhancement Homo sapiens
14 YWHAE 7531
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
15 CRK 1398
Affinity Capture-Western Homo sapiens
16 ADRB2  
Affinity Capture-Western Homo sapiens
17 SLC9A2  
Reconstituted Complex Homo sapiens
18 GFAP 2670
Affinity Capture-MS Homo sapiens
19 CD2AP 23607
Invitro Homo sapiens
20 EPS8 2059
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
21 GRB10 2887
Biochemical Activity Homo sapiens
22 RET 5979
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
23 EVL 51466
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
24 Adrb3  
Affinity Capture-Western Mus musculus
25 LRP1 4035
Biochemical Activity Homo sapiens
26 WAS 7454
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
27 AFAP1 60312
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
28 PRKCE  
Invitro Homo sapiens
29 MAPK8IP3  
Biochemical Activity Homo sapiens
30 MUC1 4582
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
31 SRC 6714
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 STAT3 6774
Invitro Homo sapiens
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
33 MST1R 4486
Invitro Homo sapiens
34 SYK 6850
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
35 YWHAG 7532
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
36 PLCG1 5335
Affinity Capture-Western Homo sapiens
37 PLD2 5338
Reconstituted Complex Homo sapiens
Biochemical Activity Homo sapiens
Affinity Capture-Western Homo sapiens
38 PLD1 5337
Biochemical Activity Homo sapiens
39 KHDRBS1 10657
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
40 TCF1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
41 YWHAH 7533
Invivo Homo sapiens
42 TYRO3  
Affinity Capture-Western Homo sapiens
43 DOCK1 1793
Affinity Capture-Western Homo sapiens
44 ERBB2 2064
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
45 ADAM12  
Invivo Homo sapiens
Invitro Homo sapiens
46 CD36 948
Invivo Homo sapiens
47 MAP2 4133
Reconstituted Complex Homo sapiens
48 YWHAB 7529
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
49 DDR2 4921
Invivo Homo sapiens
50 TNFSF11  
Affinity Capture-Western Homo sapiens
51 BCAR1 9564
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
52 FASLG 356
Invivo Homo sapiens
53 DAG1 1605
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
54 PLSCR1 5359
Biochemical Activity Homo sapiens
55 PDE6G  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
56 PDGFRB 5159
Affinity Capture-MS Homo sapiens
57 TUB  
Invivo Homo sapiens
Invitro Homo sapiens
58 CSF1R  
Affinity Capture-Western Homo sapiens
59 LYN 4067
Protein-peptide Homo sapiens
60 CDH1 999
Affinity Capture-Western Homo sapiens
61 FYN 2534
Protein-peptide Homo sapiens
62 PTK2B 2185
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
63 ARNT 405
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
64 SRF  
Invitro Homo sapiens
65 PIK3R1 5295
Invivo Homo sapiens
Affinity Capture-Western Homo sapiens
66 PTPN21  
Affinity Capture-Western Homo sapiens
67 GRIN2A  
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
68 BMX  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
69 PECAM1 5175
Affinity Capture-Western Homo sapiens
70 ASAP2 8853
Affinity Capture-Western Homo sapiens
71 ARHGAP17 55114
Reconstituted Complex Homo sapiens
72 SH3BP1  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
73 KIFAP3  
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
74 ADRB3  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
75 PRKCZ 5590
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
76 TRAF6 7189
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
77 RPS6KB1  
Biochemical Activity Homo sapiens
78 EGFR 1956
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
79 PTK2 5747
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
80 CD59 966
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
81 TRAF3 7187
Affinity Capture-Western Homo sapiens
82 RXRA  
Reconstituted Complex Homo sapiens
83 STAT1 6772
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
84 GRIN2B  
Invitro Homo sapiens
Invivo Homo sapiens
85 AHR 196
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
86 MPZL1 9019
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
87 MET 4233
Invivo Homo sapiens
88 RAF1 5894
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
89 GAB3  
Reconstituted Complex Homo sapiens
90 TRPC6 7225
Affinity Capture-Western Homo sapiens
91 TRPV4 59341
Invivo Homo sapiens
Invitro Homo sapiens
92 RPS6KB2  
Biochemical Activity Homo sapiens
93 ASAP1 50807
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
94 IL6R  
Invitro Homo sapiens
95 ITK 3702
Reconstituted Complex Homo sapiens
96 SYN1  
Invivo Homo sapiens
97 CD46 4179
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
98 GSN 2934
Affinity Capture-Western Homo sapiens
99 RASA1 5921
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
100 ESR2  
Affinity Capture-Western Homo sapiens
101 RARA 5914
Reconstituted Complex Homo sapiens
102 MYLK 4638
Invivo Homo sapiens
103 DAB2 1601
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
104 GRB2 2885
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-Western Homo sapiens
105 RICS  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
106 PELP1 27043
Affinity Capture-Western Homo sapiens
107 EPHB2 2048
Two-hybrid Homo sapiens
108 CDC37 11140
Reconstituted Complex Homo sapiens
109 ARR3 407
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
110 EFNB1 1947
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
111 FOXO1A  
Reconstituted Complex Homo sapiens
112 HSP90AA1 3320
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
113 ADAM15 8751
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
114 GAB2 9846
Invitro Homo sapiens
115 HNRNPK 3190
Affinity Capture-MS Homo sapiens
116 ESR1  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
117 SKAP1 8631
Reconstituted Complex Homo sapiens
118 DOK1 1796
Reconstituted Complex Homo sapiens
119 TRIP6  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
120 SHB  
Invivo Homo sapiens
Invitro Homo sapiens
121 GNB2L1 10399
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
122 AR 367
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
123 ANXA2 302
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
124 TRIP4  
Affinity Capture-Western Homo sapiens
125 CAV1 857
Invitro Homo sapiens
126 EP300 2033
Reconstituted Complex Homo sapiens
127 CTNND1 1500
Affinity Capture-Western Homo sapiens
128 TRAF1 7185
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which SRC is involved
PathwayEvidenceSource
Signaling by ERBB2 TAS Reactome





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