Gene description for SH3BP1
Gene name SH3-domain binding protein 1
Gene symbol SH3BP1
Other names/aliases ARHGAP43
Species Homo sapiens
 Database cross references - SH3BP1
ExoCarta ExoCarta_23616
Vesiclepedia VP_23616
Entrez Gene 23616
HGNC 10824
UniProt Q9Y3L3  
 SH3BP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
 Gene ontology annotations for SH3BP1
Molecular Function
    GTPase activator activity GO:0005096 IBA
    GTPase activator activity GO:0005096 IMP
    GTPase activator activity GO:0005096 TAS
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
    semaphorin receptor binding GO:0030215 IPI
Biological Process
    phagocytosis, engulfment GO:0006911 IMP
    actin filament organization GO:0007015 IMP
    cell migration GO:0016477 IMP
    regulation of actin filament depolymerization GO:0030834 IMP
    regulation of actin cytoskeleton organization GO:0032956 IBA
    regulation of actin cytoskeleton organization GO:0032956 IMP
    cell junction assembly GO:0034329 IMP
    regulation of Rac protein signal transduction GO:0035020 IBA
    regulation of blood vessel endothelial cell migration GO:0043535 IMP
    positive regulation of GTPase activity GO:0043547 IMP
    establishment of epithelial cell apical/basal polarity GO:0045198 IMP
    filopodium assembly GO:0046847 IEA
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    negative regulation of small GTPase mediated signal transduction GO:0051058 IBA
    negative regulation of small GTPase mediated signal transduction GO:0051058 IMP
    semaphorin-plexin signaling pathway GO:0071526 IMP
    ruffle assembly GO:0097178 IEA
Subcellular Localization
    exocyst GO:0000145 IDA
    phagocytic cup GO:0001891 IDA
    nucleus GO:0005634 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IBA
    adherens junction GO:0005912 IDA
    bicellular tight junction GO:0005923 IDA
    lamellipodium GO:0030027 IDA
    cell leading edge GO:0031252 IDA
 Experiment description of studies that identified SH3BP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for SH3BP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ARHGAP17 55114
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 NOP16 51491
Affinity Capture-MS Homo sapiens
3 TPP2 7174
Cross-Linking-MS (XL-MS) Homo sapiens
4 POT1  
Two-hybrid Homo sapiens
5 CCDC86  
Affinity Capture-MS Homo sapiens
6 PLEC 5339
Affinity Capture-MS Homo sapiens
7 EXOC8 149371
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 TIPRL 261726
Co-fractionation Homo sapiens
9 EIF6 3692
Affinity Capture-MS Homo sapiens
10 WBP11  
Affinity Capture-MS Homo sapiens
11 CRK 1398
Reconstituted Complex Homo sapiens
12 ZC3H14  
Affinity Capture-MS Homo sapiens
13 SAP18 10284
Affinity Capture-MS Homo sapiens
14 CD2AP 23607
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 PACSIN2 11252
Affinity Capture-MS Homo sapiens
16 Asap1  
Two-hybrid Mus musculus
17 CAPZA2 830
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
18 ZCCHC9  
Affinity Capture-MS Homo sapiens
19 ABL1 25
Reconstituted Complex Homo sapiens
20 CAPZA1 829
Affinity Capture-MS Homo sapiens
21 NLRP4  
Co-fractionation Homo sapiens
22 MAGEA9  
Affinity Capture-MS Homo sapiens
23 WIF1 11197
Affinity Capture-MS Homo sapiens
24 CAPZB 832
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 TERF1 7013
Two-hybrid Homo sapiens
26 ARHGAP44  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 HECW2  
Affinity Capture-MS Homo sapiens
28 NLRP3  
Co-fractionation Homo sapiens
29 WDR74 54663
Affinity Capture-MS Homo sapiens
30 KXD1 79036
Affinity Capture-MS Homo sapiens
31 TRIP10 9322
Two-hybrid Homo sapiens
32 SRC 6714
Reconstituted Complex Homo sapiens
33 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 RPS29 6235
Affinity Capture-MS Homo sapiens
35 RAC1 5879
FRET Homo sapiens
36 TRNT1 51095
Co-fractionation Homo sapiens
37 THRSP  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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