Gene description for RPS29
Gene name ribosomal protein S29
Gene symbol RPS29
Other names/aliases DBA13
S29
Species Homo sapiens
 Database cross references - RPS29
ExoCarta ExoCarta_6235
Entrez Gene 6235
HGNC 10419
MIM 603633
UniProt P62273  
 RPS29 identified in exosomes derived from the following tissue/cell type
B cells 20458337    
B cells 20458337    
Squamous carcinoma cells 20124223    
 Gene ontology annotations for RPS29
Molecular Function
    zinc ion binding GO:0008270 IDA
    structural constituent of ribosome GO:0003735 IDA
Biological Process
    translational elongation GO:0006414 TAS
    nuclear-transcribed mRNA catabolic process, nonsense-mediated decay GO:0000184 TAS
    viral process GO:0016032 TAS
    cellular protein metabolic process GO:0044267 TAS
    translation GO:0006412 TAS
    translational termination GO:0006415 TAS
    viral transcription GO:0019083 TAS
    translational initiation GO:0006413 TAS
    gene expression GO:0010467 TAS
    viral life cycle GO:0019058 TAS
    SRP-dependent cotranslational protein targeting to membrane GO:0006614 TAS
Subcellular Localization
    extracellular exosome GO:0070062 IDA
    small ribosomal subunit GO:0015935 IDA
    cytosol GO:0005829 TAS
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytoplasm GO:0005737 IDA
    focal adhesion GO:0005925 IDA
 Experiment description of studies that identified RPS29 in exosomes
1
Experiment ID 79
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
2
Experiment ID 80
ISEV standards
✘ EV Biophysical techniques
✘ EV Cytosolic markers
✔
CD81|MHCII
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20458337    
Organism Homo sapiens
Experiment description MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W.
Journal name ICB
Publication year 2010
Sample B cells
Sample name RN (HLA-DR15)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Immunobeads (MHC Class II)
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [FT-ICR]
Western blotting
3
Experiment ID 191
ISEV standards
✘ EV Biophysical techniques
✔
Alix
EV Cytosolic markers
✔
CD81|CD9
EV Membrane markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RPS29
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF4A2 1974
Affinity Capture-MS Homo sapiens
2 EXT2 2132
Affinity Capture-MS Homo sapiens
3 GC20  
Affinity Capture-MS Homo sapiens
4 DUSP24  
Affinity Capture-MS Homo sapiens
5 DDX56  
Affinity Capture-MS Homo sapiens
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