Experiment description of studies that identified RPS26 in exosomes |
1 |
Experiment ID |
79 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD81|MHCII
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20458337
|
Organism |
Homo sapiens |
Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1 |
Authors |
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. |
Journal name |
ICB
|
Publication year |
2010 |
Sample |
B cells |
Sample name |
RN (HLA-DR15) |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [FT-ICR] Western blotting |
|
|
2 |
Experiment ID |
80 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD81|MHCII
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20458337
|
Organism |
Homo sapiens |
Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2 |
Authors |
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. |
Journal name |
ICB
|
Publication year |
2010 |
Sample |
B cells |
Sample name |
RN (HLA-DR15) |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [FT-ICR] Western blotting |
|
|
3 |
Experiment ID |
81 |
ISEV standards |
✘
|
EV Biophysical techniques |
✘
|
EV Cytosolic markers |
✔
CD81|MHCII
|
EV Membrane markers |
✘
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
20458337
|
Organism |
Homo sapiens |
Experiment description |
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3 |
Authors |
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W. |
Journal name |
ICB
|
Publication year |
2010 |
Sample |
B cells |
Sample name |
RN (HLA-DR15) |
Isolation/purification methods |
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II) |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry [FT-ICR] Western blotting |
|
|
4 |
Experiment ID |
282 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Ultracentrifugation - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
5 |
Experiment ID |
283 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Ultracentrifugation - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
6 |
Experiment ID |
285 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 1 |
Isolation/purification methods |
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
7 |
Experiment ID |
286 |
ISEV standards |
✔
CEM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81|EpCAM
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
DLS
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25890246
|
Organism |
Homo sapiens |
Experiment description |
Highly-purified exosomes and shed microvesicles isolated from the human colon cancer cell line LIM1863 by sequential centrifugal ultrafiltration are biochemically and functionally distinct. |
Authors |
Xu R, Greening DW, Rai A, Ji H, Simpson RJ. |
Journal name |
Methods
|
Publication year |
2015 |
Sample |
Colorectal cancer cells |
Sample name |
LIM1863 - Sequential centrifugal ultrafiltration - Rep 2 |
Isolation/purification methods |
Differential centrifugation Filtration Sequential centrifugal ultrafiltration Centrifugal concentration |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
8 |
Experiment ID |
224 |
ISEV standards |
✔
EM|AFM
|
EV Biophysical techniques |
✔
Alix|TSG101
|
EV Cytosolic markers |
✔
CD63|CD81
|
EV Membrane markers |
✔
GOLGA2
|
EV Negative markers |
✘
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
25944692
|
Organism |
Homo sapiens |
Experiment description |
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes |
Authors |
Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S |
Journal name |
Oncotarget
|
Publication year |
2015 |
Sample |
Neuroblastoma cells |
Sample name |
SH-SY5Y |
Isolation/purification methods |
Differential centrifugation Ultracentrifugation OptiPrep density gradient |
Flotation density |
1.10 g/mL
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry Western blotting |
|
|
9 |
Experiment ID |
217 |
ISEV standards |
✔
EM
|
EV Biophysical techniques |
✔
TSG101
|
EV Cytosolic markers |
✔
CD81|CD9|CD63
|
EV Membrane markers |
✘
|
EV Negative markers |
✔
NTA
|
EV Particle analysis
|
|
Identified molecule |
protein
|
Identification method |
Mass spectrometry
|
PubMed ID |
23844026
|
Organism |
Homo sapiens |
Experiment description |
Characterization of human thymic exosomes. |
Authors |
Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O. |
Journal name |
PLoS One
|
Publication year |
2013 |
Sample |
Thymus |
Sample name |
Normal-Thymus |
Isolation/purification methods |
Differential centrifugation Filtration Ultracentrifugation |
Flotation density |
-
|
Molecules identified in the study |
Protein |
Methods used in the study |
Mass spectrometry |
|
|