Gene description for B3GNT2
Gene name UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 2
Gene symbol B3GNT2
Other names/aliases B3GN-T2
B3GNT
B3GNT-2
B3GNT1
BETA3GNT
BGNT2
BGnT-2
Species Homo sapiens
 Database cross references - B3GNT2
ExoCarta ExoCarta_10678
Entrez Gene 10678
HGNC 15629
MIM 605581
UniProt Q9NY97  
 B3GNT2 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for B3GNT2
Molecular Function
    galactosyltransferase activity GO:0008378 IEA
    N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity GO:0008532 IDA
Biological Process
    keratan sulfate metabolic process GO:0042339 TAS
    axon guidance GO:0007411 IEA
    cellular protein metabolic process GO:0044267 TAS
    O-glycan processing GO:0016266 TAS
    carbohydrate metabolic process GO:0005975 TAS
    glycosaminoglycan metabolic process GO:0030203 TAS
    keratan sulfate biosynthetic process GO:0018146 TAS
    protein O-linked glycosylation GO:0006493 TAS
    poly-N-acetyllactosamine biosynthetic process GO:0030311 IDA
    sensory perception of smell GO:0007608 IEA
    post-translational protein modification GO:0043687 TAS
    small molecule metabolic process GO:0044281 TAS
Subcellular Localization
    integral component of membrane GO:0016021 IEA
    extracellular exosome GO:0070062 IDA
    Golgi membrane GO:0000139 TAS
 Experiment description of studies that identified B3GNT2 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for B3GNT2
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which B3GNT2 is involved
PathwayEvidenceSource
Keratan sulfate biosynthesis TAS Reactome
O-linked glycosylation of mucins TAS Reactome





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