Gene description for DNASE1L1
Gene name deoxyribonuclease I-like 1
Gene symbol DNASE1L1
Other names/aliases DNAS1L1
DNASEX
DNL1L
G4.8
XIB
Species Homo sapiens
 Database cross references - DNASE1L1
ExoCarta ExoCarta_1774
Vesiclepedia VP_1774
Entrez Gene 1774
HGNC 2957
MIM 300081
UniProt P49184  
 DNASE1L1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Mesenchymal stem cells Unpublished / Not applicable
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for DNASE1L1
Molecular Function
    DNA binding GO:0003677 IBA
    deoxyribonuclease I activity GO:0004530 IBA
    DNA nuclease activity GO:0004536 TAS
    protein binding GO:0005515 IPI
Biological Process
    DNA metabolic process GO:0006259 TAS
    DNA catabolic process GO:0006308 IBA
Subcellular Localization
    extracellular region GO:0005576 TAS
    nucleus GO:0005634 IBA
    endoplasmic reticulum GO:0005783 IDA
    specific granule lumen GO:0035580 TAS
 Experiment description of studies that identified DNASE1L1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 126
MISEV standards
✘ Biophysical techniques
✔
GAPDH
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry [LTQ-FT Ultra]
PubMed ID Unpublished / Not applicable
Organism Homo sapiens
Experiment description Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors "Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name Mesenchymal Stem Cell Therapy
Publication year 2011
Sample Mesenchymal stem cells
Sample name huES9.E1
Isolation/purification methods HPLC
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Antobody array
Mass spectrometry
7
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
8
Experiment ID 211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DNASE1L1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 UGGT2 55757
Affinity Capture-MS Homo sapiens
2 CNTNAP3  
Affinity Capture-MS Homo sapiens
3 CACNA2D2 9254
Affinity Capture-MS Homo sapiens
4 IDUA 3425
Affinity Capture-MS Homo sapiens
5 CHST14 113189
Affinity Capture-MS Homo sapiens
6 GFAP 2670
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
7 GPR110  
Affinity Capture-MS Homo sapiens
8 THSD4 79875
Affinity Capture-MS Homo sapiens
9 POMGNT2  
Affinity Capture-MS Homo sapiens
10 TRIM54  
Two-hybrid Homo sapiens
11 FGFR3 2261
Affinity Capture-MS Homo sapiens
12 RPS6KA3 6197
Affinity Capture-MS Homo sapiens
13 ITGA7 3679
Affinity Capture-MS Homo sapiens
14 B3GNT2 10678
Affinity Capture-MS Homo sapiens
15 PLAU 5328
Affinity Capture-MS Homo sapiens
16 CEMIP 57214
Affinity Capture-MS Homo sapiens
17 HYAL2 8692
Affinity Capture-MS Homo sapiens
18 CD109 135228
Affinity Capture-MS Homo sapiens
19 SEL1L 6400
Affinity Capture-MS Homo sapiens
20 CRELD2 79174
Affinity Capture-MS Homo sapiens
21 FGFR4 2264
Affinity Capture-MS Homo sapiens
22 ST14 6768
Affinity Capture-MS Homo sapiens
23 SUN2 25777
Affinity Capture-MS Homo sapiens
24 NLGN2 57555
Affinity Capture-MS Homo sapiens
25 DGCR2 9993
Affinity Capture-MS Homo sapiens
26 ST8SIA4 7903
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
27 SEC11C 90701
Affinity Capture-MS Homo sapiens
28 GALNT18  
Affinity Capture-MS Homo sapiens
29 IL17RA 23765
Affinity Capture-MS Homo sapiens
30 TMEM2 23670
Affinity Capture-MS Homo sapiens
31 KIF14 9928
Affinity Capture-MS Homo sapiens
32 TAF5L  
Affinity Capture-MS Homo sapiens
33 MICA 100507436
Affinity Capture-MS Homo sapiens
34 SEMA3B 7869
Affinity Capture-MS Homo sapiens
35 RPS4Y2 140032
Affinity Capture-MS Homo sapiens
36 BANP  
Affinity Capture-MS Homo sapiens
37 POMGNT1 55624
Affinity Capture-MS Homo sapiens
38 TOR1B 27348
Affinity Capture-MS Homo sapiens
39 LRIG2  
Affinity Capture-MS Homo sapiens
40 IGF1R 3480
Affinity Capture-MS Homo sapiens
41 CLSTN1 22883
Affinity Capture-MS Homo sapiens
42 HSP90B1 7184
Affinity Capture-MS Homo sapiens
43 CHPF2 54480
Affinity Capture-MS Homo sapiens
44 CSPG4 1464
Affinity Capture-MS Homo sapiens
45 MAN2A2 4122
Affinity Capture-MS Homo sapiens
46 GLB1L 79411
Affinity Capture-MS Homo sapiens
47 PCSK9 255738
Affinity Capture-MS Homo sapiens
48 B4GALT3 8703
Affinity Capture-MS Homo sapiens
49 ART5  
Affinity Capture-MS Homo sapiens
50 PLXNA3 55558
Affinity Capture-MS Homo sapiens
51 TMEM131  
Affinity Capture-MS Homo sapiens
52 ICAM5 7087
Affinity Capture-MS Homo sapiens
53 ATRN 8455
Affinity Capture-MS Homo sapiens
54 MESDC2 23184
Affinity Capture-MS Homo sapiens
55 MEGF8 1954
Affinity Capture-MS Homo sapiens
56 LRRC15 131578
Affinity Capture-MS Homo sapiens
57 HSPA5 3309
Affinity Capture-MS Homo sapiens
58 QSOX1 5768
Affinity Capture-MS Homo sapiens
59 RGL3  
Affinity Capture-MS Homo sapiens
60 MAN2B2 23324
Affinity Capture-MS Homo sapiens
61 ICAM1 3383
Affinity Capture-MS Homo sapiens
62 METRNL 284207
Affinity Capture-MS Homo sapiens
63 ABHD12 26090
Affinity Capture-MS Homo sapiens
64 PLXNB2 23654
Affinity Capture-MS Homo sapiens
65 SEMA6B 10501
Affinity Capture-MS Homo sapiens
66 PLXND1 23129
Affinity Capture-MS Homo sapiens
67 GANAB 23193
Affinity Capture-MS Homo sapiens
68 TMEM132A 54972
Affinity Capture-MS Homo sapiens
69 CRLF1 9244
Affinity Capture-MS Homo sapiens
70 CHRD  
Affinity Capture-MS Homo sapiens
71 HLA-F 3134
Affinity Capture-MS Homo sapiens
72 MSLN 10232
Affinity Capture-MS Homo sapiens
73 HLA-DQB1 3119
Affinity Capture-MS Homo sapiens
74 SENP5  
Affinity Capture-MS Homo sapiens
75 BMP1 649
Affinity Capture-MS Homo sapiens
76 HLA-A 3105
Affinity Capture-MS Homo sapiens
77 TTC13  
Affinity Capture-MS Homo sapiens
78 ADAMTS7  
Affinity Capture-MS Homo sapiens
79 MR1  
Affinity Capture-MS Homo sapiens
80 ADAM17 6868
Affinity Capture-MS Homo sapiens
81 TTC17 55761
Affinity Capture-MS Homo sapiens
82 FOXRED2  
Affinity Capture-MS Homo sapiens
83 METRN 79006
Affinity Capture-MS Homo sapiens
84 PDIA4 9601
Affinity Capture-MS Homo sapiens
85 KDELC1 79070
Affinity Capture-MS Homo sapiens
86 SEC11A 23478
Affinity Capture-MS Homo sapiens
87 ADAMTS14  
Affinity Capture-MS Homo sapiens
88 PLXNA1 5361
Affinity Capture-MS Homo sapiens
89 SEMA4G  
Affinity Capture-MS Homo sapiens
90 SIL1 64374
Affinity Capture-MS Homo sapiens
91 PPOX 5498
Affinity Capture-MS Homo sapiens
92 INSR 3643
Affinity Capture-MS Homo sapiens
93 GPR107 57720
Affinity Capture-MS Homo sapiens
94 NDUFAF7  
Affinity Capture-MS Homo sapiens
95 BDNF 627
Affinity Capture-MS Homo sapiens
96 OS9 10956
Affinity Capture-MS Homo sapiens
97 DDX58 23586
Affinity Capture-RNA Homo sapiens
98 EDEM2  
Affinity Capture-MS Homo sapiens
99 PGAM2 5224
Affinity Capture-MS Homo sapiens
100 COL6A2 1292
Affinity Capture-MS Homo sapiens
101 ITGA2 3673
Affinity Capture-MS Homo sapiens
102 FUT11 170384
Affinity Capture-MS Homo sapiens
103 SPCS1 28972
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 B4GALT4 8702
Affinity Capture-MS Homo sapiens
105 RPL23 9349
Affinity Capture-MS Homo sapiens
106 LAMB2 3913
Affinity Capture-MS Homo sapiens
107 GPC3 2719
Affinity Capture-MS Homo sapiens
108 OLFM2  
Affinity Capture-MS Homo sapiens
109 LAMA5 3911
Affinity Capture-MS Homo sapiens
110 PTPRK 5796
Affinity Capture-MS Homo sapiens
111 CLPTM1L 81037
Affinity Capture-MS Homo sapiens
112 ANKRD46 157567
Affinity Capture-MS Homo sapiens
113 SPCS2 9789
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
114 HLA-C 3107
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which DNASE1L1 is involved
PathwayEvidenceSource
Immune System TAS Reactome
Innate Immune System TAS Reactome
Neutrophil degranulation TAS Reactome





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