Gene description for RPS6KA3
Gene name ribosomal protein S6 kinase, 90kDa, polypeptide 3
Gene symbol RPS6KA3
Other names/aliases CLS
HU-3
ISPK-1
MAPKAPK1B
MRX19
RSK
RSK2
S6K-alpha3
p90-RSK2
pp90RSK2
Species Homo sapiens
 Database cross references - RPS6KA3
ExoCarta ExoCarta_6197
Entrez Gene 6197
HGNC 10432
MIM 300075
UniProt P51812  
 RPS6KA3 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Hepatocytes 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Squamous carcinoma cells 20124223    
Thymus 23844026    
 Gene ontology annotations for RPS6KA3
Molecular Function
    protein kinase binding GO:0019901 IEA
    ribosomal protein S6 kinase activity GO:0004711 IBA
    ATP binding GO:0005524 IEA
    protein kinase activity GO:0004672 TAS
    protein binding GO:0005515 IPI
    magnesium ion binding GO:0000287 IEA
    protein serine/threonine kinase activity GO:0004674 TAS
    cysteine-type endopeptidase inhibitor activity involved in apoptotic process GO:0043027 IDA
Biological Process
    positive regulation of cell differentiation GO:0045597 TAS
    central nervous system development GO:0007417 TAS
    regulation of translation in response to stress GO:0043555 TAS
    negative regulation of cysteine-type endopeptidase activity involved in apoptotic process GO:0043154 IDA
    positive regulation of cell growth GO:0030307 TAS
    regulation of DNA-templated transcription in response to stress GO:0043620 TAS
    toll-like receptor TLR6:TLR2 signaling pathway GO:0038124 TAS
    neurotrophin TRK receptor signaling pathway GO:0048011 TAS
    toll-like receptor 3 signaling pathway GO:0034138 TAS
    innate immune response GO:0045087 TAS
    intracellular signal transduction GO:0035556 IBA
    toll-like receptor 10 signaling pathway GO:0034166 TAS
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IMP
    MyD88-independent toll-like receptor signaling pathway GO:0002756 TAS
    synaptic transmission GO:0007268 TAS
    toll-like receptor 5 signaling pathway GO:0034146 TAS
    signal transduction GO:0007165 TAS
    skeletal system development GO:0001501 TAS
    toll-like receptor TLR1:TLR2 signaling pathway GO:0038123 TAS
    cell cycle GO:0007049 IEA
    toll-like receptor 4 signaling pathway GO:0034142 TAS
    stress-activated MAPK cascade GO:0051403 TAS
    toll-like receptor 2 signaling pathway GO:0034134 TAS
    TRIF-dependent toll-like receptor signaling pathway GO:0035666 TAS
    MyD88-dependent toll-like receptor signaling pathway GO:0002755 TAS
    protein phosphorylation GO:0006468 IBA
    axon guidance GO:0007411 TAS
    toll-like receptor signaling pathway GO:0002224 TAS
    negative regulation of apoptotic process GO:0043066 TAS
    toll-like receptor 9 signaling pathway GO:0034162 TAS
    response to lipopolysaccharide GO:0032496 ISS
Subcellular Localization
    cytosol GO:0005829 TAS
    cytoplasm GO:0005737 IBA
    nucleoplasm GO:0005654 TAS
 Experiment description of studies that identified RPS6KA3 in exosomes
1
Experiment ID 207
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
EM
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
EV Biophysical techniques
TSG101|HSP70
EV Cytosolic markers
FLOT1
EV Membrane markers
VDAC
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 237
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
5
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 191
ISEV standards
EV Biophysical techniques
Alix
EV Cytosolic markers
CD81|CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 20124223    
Organism Homo sapiens
Experiment description Hypoxic tumor cell modulates its microenvironment to enhance angiogenic and metastatic potential by secretion of proteins and exosomes.
Authors Park JE, Tan HS, Datta A, Lai RC, Zhang H, Meng W, Lim SK, Sze SK.
Journal name Mol Cell Proteomics
Publication year 2010
Sample Squamous carcinoma cells
Sample name Squamous carcinoma cell (A431)
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 217
ISEV standards
EM
EV Biophysical techniques
TSG101
EV Cytosolic markers
CD81|CD9|CD63
EV Membrane markers
EV Negative markers
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for RPS6KA3
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PEA15  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
2 MAPK1 5594
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
3 MAPK3 5595
Affinity Capture-Western Homo sapiens
4 CREBBP  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Phenotypic Suppression Homo sapiens
5 PDPK1  
Affinity Capture-Western Homo sapiens
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