Gene description for GBA
Gene name glucosidase, beta, acid
Gene symbol GBA
Other names/aliases GBA1
GCB
GLUC
Species Homo sapiens
 Database cross references - GBA
ExoCarta ExoCarta_2629
Vesiclepedia VP_2629
Entrez Gene 2629
HGNC 4177
MIM 606463
UniProt P04062  
 GBA identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Chondrocytes 35931686    
Chondrocytes 35931686    
Colorectal cancer cells 34887515    
Mesenchymal stem cells 36408942    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for GBA
Molecular Function
    galactosylceramidase activity GO:0004336 IEA
    glucosylceramidase activity GO:0004348 IBA
    glucosylceramidase activity GO:0004348 IDA
    glucosylceramidase activity GO:0004348 IMP
    signaling receptor binding GO:0005102 ISS
    scavenger receptor binding GO:0005124 IPI
    protein binding GO:0005515 IPI
    glucosyltransferase activity GO:0046527 IDA
    steryl-beta-glucosidase activity GO:0050295 IDA
Biological Process
    mitophagy GO:0000423 IEA
    glucosylceramide catabolic process GO:0006680 IBA
    glucosylceramide catabolic process GO:0006680 IDA
    glucosylceramide catabolic process GO:0006680 IMP
    autophagy GO:0006914 IMP
    mitochondrion organization GO:0007005 IMP
    lysosome organization GO:0007040 IMP
    cholesterol metabolic process GO:0008203 IDA
    cholesterol metabolic process GO:0008203 IEA
    determination of adult lifespan GO:0008340 IEA
    cellular response to starvation GO:0009267 IEA
    response to pH GO:0009268 IEA
    microglia differentiation GO:0014004 IEA
    regulation of macroautophagy GO:0016241 TAS
    antigen processing and presentation GO:0019882 IEA
    lipid storage GO:0019915 IEA
    cerebellar Purkinje cell layer formation GO:0021694 IEA
    pyramidal neuron differentiation GO:0021859 IEA
    respiratory electron transport chain GO:0022904 IEA
    termination of signal transduction GO:0023021 IMP
    lipid glycosylation GO:0030259 IDA
    neuron projection development GO:0031175 IMP
    negative regulation of protein-containing complex assembly GO:0031333 IDA
    regulation of TOR signaling GO:0032006 IMP
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IEA
    negative regulation of interleukin-6 production GO:0032715 IDA
    T cell differentiation in thymus GO:0033077 IEA
    response to testosterone GO:0033574 IEA
    proteasome-mediated ubiquitin-dependent protein catabolic process GO:0043161 IEA
    positive regulation of protein-containing complex disassembly GO:0043243 IDA
    negative regulation of MAPK cascade GO:0043409 IMP
    negative regulation of neuron apoptotic process GO:0043524 IEA
    response to estrogen GO:0043627 IEA
    sphingosine biosynthetic process GO:0046512 IMP
    ceramide biosynthetic process GO:0046513 IMP
    cell maturation GO:0048469 IEA
    brain morphogenesis GO:0048854 IEA
    homeostasis of number of cells GO:0048872 IEA
    negative regulation of inflammatory response GO:0050728 IC
    neuromuscular process GO:0050905 IEA
    negative regulation of protein metabolic process GO:0051248 IEA
    neuron apoptotic process GO:0051402 IEA
    establishment of skin barrier GO:0061436 IEA
    microglial cell proliferation GO:0061518 IEA
    motor behavior GO:0061744 IEA
    cellular response to tumor necrosis factor GO:0071356 IMP
    hematopoietic stem cell proliferation GO:0071425 IEA
    response to dexamethasone GO:0071548 IEA
    lymphocyte migration GO:0072676 IEA
    response to thyroid hormone GO:0097066 IEA
    beta-glucoside catabolic process GO:1901805 IEA
    positive regulation of protein lipidation GO:1903061 IGI
    positive regulation of neuronal action potential GO:1904457 IMP
    autophagosome organization GO:1905037 IEA
    positive regulation of type 2 mitophagy GO:1905091 IEA
    regulation of lysosomal protein catabolic process GO:1905165 TAS
Subcellular Localization
    lysosome GO:0005764 IMP
    lysosomal membrane GO:0005765 HDA
    lysosomal membrane GO:0005765 IDA
    lysosomal membrane GO:0005765 ISS
    lysosomal membrane GO:0005765 TAS
    endoplasmic reticulum GO:0005783 ISS
    Golgi apparatus GO:0005794 ISS
    trans-Golgi network GO:0005802 ISS
    lysosomal lumen GO:0043202 ISS
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified GBA in sEVs
1
Experiment ID 489
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 497
MISEV standards
✘ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 498
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35931686    
Organism Homo sapiens
Experiment description Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors "Varela-Eirín M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, García-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name Cell Death Dis
Publication year 2022
Sample Chondrocytes
Sample name T/C-28a2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
7
Experiment ID 1203
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors "Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
8
Experiment ID 488
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 211
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
10
Experiment ID 212
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors "Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
11
Experiment ID 834
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
12
Experiment ID 835
MISEV standards
✔ Biophysical techniques
✔ Enriched markers
✔ Negative markers
✔ Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for GBA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 LAIR2  
Affinity Capture-MS Homo sapiens
2 CCT3 7203
Affinity Capture-MS Homo sapiens
3 HSPA6 3310
Affinity Capture-MS Homo sapiens
4 DHCR24 1718
Affinity Capture-MS Homo sapiens
5 RIN3  
Affinity Capture-MS Homo sapiens
6 MYLK2 85366
Affinity Capture-MS Homo sapiens
7 FMR1 2332
Affinity Capture-MS Homo sapiens
8 JPH1 56704
Affinity Capture-MS Homo sapiens
9 GFAP 2670
Affinity Capture-MS Homo sapiens
10 TUBB8 347688
Affinity Capture-MS Homo sapiens
11 C9orf78 51759
Affinity Capture-MS Homo sapiens
12 VPS13C 54832
Affinity Capture-MS Homo sapiens
13 CFAP44 55779
Affinity Capture-MS Homo sapiens
14 ARL8B 55207
Affinity Capture-MS Homo sapiens
15 PDE2A  
Affinity Capture-MS Homo sapiens
16 HCCS 3052
Affinity Capture-MS Homo sapiens
17 IRX5  
Affinity Capture-MS Homo sapiens
18 RPS6KA3 6197
Affinity Capture-MS Homo sapiens
19 APOC3 345
Affinity Capture-MS Homo sapiens
20 SPIN1  
Affinity Capture-MS Homo sapiens
21 IMP3 55272
Affinity Capture-MS Homo sapiens
22 B4GALT7 11285
Affinity Capture-MS Homo sapiens
23 ZMYND11 10771
Affinity Capture-MS Homo sapiens
24 VPS13D 55187
Affinity Capture-MS Homo sapiens
25 RHBDF1 64285
Affinity Capture-MS Homo sapiens
26 COMT 1312
Affinity Capture-MS Homo sapiens
27 NUDCD1 84955
Affinity Capture-Luminescence Homo sapiens
Affinity Capture-MS Homo sapiens
28 FGD6  
Affinity Capture-MS Homo sapiens
29 INADL 10207
Affinity Capture-MS Homo sapiens
30 POTEE 445582
Affinity Capture-MS Homo sapiens
31 SLC12A7 10723
Affinity Capture-MS Homo sapiens
32 NEDD4 4734
Biochemical Activity Homo sapiens
33 EEA1 8411
Affinity Capture-MS Homo sapiens
34 TCP1 6950
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
35 TOP3B 8940
Affinity Capture-MS Homo sapiens
36 RAB14 51552
Affinity Capture-MS Homo sapiens
37 PARK2  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
38 RAB21 23011
Affinity Capture-MS Homo sapiens
39 HSP90B1 7184
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
40 CBL 867
Affinity Capture-Western Homo sapiens
41 PLOD2 5352
Co-fractionation Homo sapiens
42 MYDGF 56005
Affinity Capture-MS Homo sapiens
43 SDF2L1 23753
Affinity Capture-MS Homo sapiens
44 CTSC 1075
Affinity Capture-MS Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 POTEM 641455
Affinity Capture-MS Homo sapiens
47 OPA1 4976
Affinity Capture-MS Homo sapiens
48 HSD3B1  
Affinity Capture-MS Homo sapiens
49 RDH11 51109
Affinity Capture-MS Homo sapiens
50 BMP2K 55589
Affinity Capture-MS Homo sapiens
51 ZFP91 80829
Affinity Capture-MS Homo sapiens
52 TUBA1B 10376
Affinity Capture-MS Homo sapiens
53 ATP6V1B1 525
Affinity Capture-MS Homo sapiens
54 TMEM192 201931
Affinity Capture-MS Homo sapiens
55 ITCH 83737
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
56 SEC61B 10952
Proximity Label-MS Homo sapiens
57 DAXX  
Affinity Capture-MS Homo sapiens
58 PCBP2 5094
Co-fractionation Homo sapiens
59 ZNF175  
Affinity Capture-MS Homo sapiens
60 ZCCHC8 55596
Affinity Capture-MS Homo sapiens
61 C17orf104  
Affinity Capture-MS Homo sapiens
62 HNRNPD 3184
Affinity Capture-MS Homo sapiens
63 OGT 8473
Reconstituted Complex Homo sapiens
64 KIAA1462 57608
Affinity Capture-MS Homo sapiens
65 TUBB2A 7280
Affinity Capture-MS Homo sapiens
66 IAH1 285148
Affinity Capture-MS Homo sapiens
67 CANX 821
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
68 MYH8 4626
Affinity Capture-MS Homo sapiens
69 PLD4  
Affinity Capture-MS Homo sapiens
70 TUBB4B 10383
Affinity Capture-MS Homo sapiens
71 FBXO6 26270
Affinity Capture-MS Homo sapiens
72 RIPK4  
Affinity Capture-MS Homo sapiens
73 DGKZ  
Affinity Capture-MS Homo sapiens
74 RPA3 6119
Proximity Label-MS Homo sapiens
75 UGT2B15  
Affinity Capture-MS Homo sapiens
76 HSPA1A 3303
Affinity Capture-MS Homo sapiens
77 EEF1A2 1917
Affinity Capture-MS Homo sapiens
78 HSPA4 3308
Affinity Capture-Western Homo sapiens
79 SKIV2L2 23517
Affinity Capture-MS Homo sapiens
80 HYOU1 10525
Affinity Capture-MS Homo sapiens
81 MYH13 8735
Affinity Capture-MS Homo sapiens
82 HSPD1 3329
Affinity Capture-MS Homo sapiens
83 SPDYE4  
Affinity Capture-MS Homo sapiens
84 SETDB1  
Affinity Capture-MS Homo sapiens
85 GBAP1  
Affinity Capture-MS Homo sapiens
86 RAB27A 5873
Affinity Capture-MS Homo sapiens
87 RAE1 8480
Affinity Capture-MS Homo sapiens
88 FMR1NB  
Affinity Capture-MS Homo sapiens
89 DENND4C 55667
Affinity Capture-MS Homo sapiens
90 SNCA 6622
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
91 CEP290  
Affinity Capture-MS Homo sapiens
92 FASN 2194
Positive Genetic Homo sapiens
93 CCT8 10694
Affinity Capture-MS Homo sapiens
94 GPBP1  
Affinity Capture-MS Homo sapiens
95 SNX25 83891
Affinity Capture-MS Homo sapiens
96 SVIL 6840
Affinity Capture-MS Homo sapiens
97 ATP5J 522
Affinity Capture-MS Homo sapiens
98 ZNF273  
Affinity Capture-MS Homo sapiens
99 RAB27B 5874
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
100 TUBA4B 80086
Affinity Capture-MS Homo sapiens
101 CNPY2 10330
Affinity Capture-MS Homo sapiens
102 SCAMP3 10067
Affinity Capture-MS Homo sapiens
103 TUBB3 10381
Affinity Capture-MS Homo sapiens
104 NHLRC2 374354
Affinity Capture-MS Homo sapiens
105 GBA 2629
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
106 RNF20 56254
Affinity Capture-MS Homo sapiens
107 HSP90AA1 3320
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
108 MKI67  
Affinity Capture-MS Homo sapiens
109 TRIP12 9320
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
110 TUBA3E 112714
Affinity Capture-MS Homo sapiens
111 FHAD1 114827
Affinity Capture-MS Homo sapiens
112 POMK  
Affinity Capture-MS Homo sapiens
113 ERP27  
Affinity Capture-MS Homo sapiens
114 SCARB2 950
Affinity Capture-Western Homo sapiens
115 ESYT1 23344
Affinity Capture-MS Homo sapiens
116 GUCA1B  
Affinity Capture-MS Homo sapiens
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