Gene ontology annotations for TUBB4B
Experiment description of studies that identified TUBB4B in sEVs
1
Experiment ID
79
MISEV standards
✘
Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 1
Authors
"Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
2
Experiment ID
80
MISEV standards
✘
Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis -Sample 2
Authors
"Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
3
Experiment ID
81
MISEV standards
✘
Biophysical techniques
✔
CD81|MHCII
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20458337
Organism
Homo sapiens
Experiment description
MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis - Sample 3
Authors
"Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W."
Journal name
ICB
Publication year
2010
Sample
B cells
Sample name
RN (HLA-DR15)
Isolation/purification methods
Differential centrifugation Sucrose density gradient Immunobeads (MHC Class II)
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [FT-ICR] Western blotting
4
Experiment ID
76
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH|HSP90|CD81|CD9|CD63|LAMP1|MHCI
Enriched markers
✔
HSP90B1
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
20224111
Organism
Homo sapiens
Experiment description
Proteomics analysis of bladder cancer exosomes.
Authors
"Welton JL, Khanna S, Giles PJ, Brennan P, Brewis IA, Staffurth J, Mason MD, Clayton A."
Journal name
MCP
Publication year
2010
Sample
Bladder cancer cells
Sample name
HT1376
Isolation/purification methods
Differential centrifugation Sucrose density gradient
Flotation density
1.10-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF/TOF] Western blotting FACS
5
Experiment ID
489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 6
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
6
Experiment ID
490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
7
Experiment ID
491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 8
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
8
Experiment ID
492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36408942
Organism
Rattus norvegicus
Experiment description
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors
"Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name
Proteomics
Publication year
2023
Sample
Bone marrow mesenchymal stem cells
Sample name
BMSC - Passage 9
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
9
Experiment ID
412
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MCF7
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
10
Experiment ID
414
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
11
Experiment ID
426
MISEV standards
✘
Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
12
Experiment ID
427
MISEV standards
✘
Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Breast cancer cells
Sample name
MDA-MB-231 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
13
Experiment ID
1221
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
31054213
Organism
Homo sapiens
Experiment description
Phosphoproteome Profiling of Isogenic Cancer Cell-Derived Exosome Reveals HSP90 as a Potential Marker for Human Cholangiocarcinoma
Authors
"Weeraphan C, Phongdara A, Chaiyawat P, Diskul-Na-Ayudthaya P, Chokchaichamnankit D, Verathamjamras C, Netsirisawan P, Yingchutrakul Y, Roytrakul S, Champattanachai V, Svasti J, Srisomsap C."
Journal name
Proteomics
Publication year
2019
Sample
Cholangiocarcinoma cells
Sample name
KKU-M213
Isolation/purification methods
Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Sucrose density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
14
Experiment ID
1222
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|GAPDH
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
31054213
Organism
Homo sapiens
Experiment description
Phosphoproteome Profiling of Isogenic Cancer Cell-Derived Exosome Reveals HSP90 as a Potential Marker for Human Cholangiocarcinoma
Authors
"Weeraphan C, Phongdara A, Chaiyawat P, Diskul-Na-Ayudthaya P, Chokchaichamnankit D, Verathamjamras C, Netsirisawan P, Yingchutrakul Y, Roytrakul S, Champattanachai V, Svasti J, Srisomsap C."
Journal name
Proteomics
Publication year
2019
Sample
Cholangiocarcinoma cells
Sample name
KKU-M213D5
Isolation/purification methods
Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation Sucrose density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
15
Experiment ID
494
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD151|CD63|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
Osteoarthritic cartilage
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
16
Experiment ID
496
MISEV standards
✘
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|SDCBP|TFRC|TSG101
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
Healthy cartilage
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
17
Experiment ID
497
MISEV standards
✘
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
18
Experiment ID
498
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD151|CD63|CD81|CD82|FLOT1|FLOT2|GAPDH|LAMP1|LAMP2|RAB5A|SDCBP|TFRC|TSG101|UCHL1
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35931686
Organism
Homo sapiens
Experiment description
Extracellular vesicles enriched in connexin 43 promote a senescent phenotype in bone and synovial cells contributing to osteoarthritis progression
Authors
"Varela-EirÃÂn M, Carpintero-Fernández P, Guitián-Caamaño A, Varela-Vázquez A, GarcÃÂa-Yuste A, Sánchez-Temprano A, Bravo-López SB, Yañez-Cabanas J, Fonseca E, Largo R, Mobasheri A, Caeiro JR, Mayán MD."
Journal name
Cell Death Dis
Publication year
2022
Sample
Chondrocytes
Sample name
T/C-28a2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectometry
19
Experiment ID
20
MISEV standards
✔
EM
Biophysical techniques
✔
HSP90|CD63|CD81|LAMP1
Enriched markers
✔
GOLGA2|cytochrome c
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
17956143
Organism
Homo sapiens
Experiment description
Proteomic analysis of microvesicles derived from human colorectal cancer cells.
Authors
"Choi DS, Lee JM, Park GW, Lim HW, Bang JY, Kim YK, Kwon KH, Kwon HJ, Kim KP, Gho YS"
Journal name
JPR
Publication year
2007
Sample
Colorectal cancer cells
Sample name
HT29
Isolation/purification methods
Differential centrifugation Sucrose density gradient Diafiltration
Flotation density
1.16 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [LTQ] Western blotting
20
Experiment ID
21
MISEV standards
✔
EM|IEM
Biophysical techniques
✔
Alix|TSG101|HSP70|CD63
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
19837982
Organism
Homo sapiens
Experiment description
Proteomic and bioinformatic analysis of immunoaffinity-purified exosomes derived from the human colon tumor cell line LIM1215.
Authors
"Suresh Mathivanan, Justin W.E. Lim, Bow J. Tauro, Hong Ji, Robert L. Moritz and Richard J. Simpson"
Journal name
MCP
Publication year
2009
Sample
Colorectal cancer cells
Sample name
LIM1215
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.10-1.12 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [Orbitrap] Western blotting
21
Experiment ID
1203
MISEV standards
✔
EM
Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
34887515
Organism
Homo sapiens
Experiment description
Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors
"Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Colorectal cancer cells
Sample name
DiFi
Isolation/purification methods
Differential centrifugation Filtration Centrifugal ultrafiltration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein miRNA
Methods used in the study
Western blotting Mass spectrometry RNA sequencing
22
Experiment ID
407
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|TSG101|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
23
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
24
Experiment ID
419
MISEV standards
✘
Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
25
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
26
Experiment ID
420
MISEV standards
✘
Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Embryonic kidney cells
Sample name
HEK293T - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
27
Experiment ID
1275
MISEV standards
Biophysical techniques
✔
CD9|TSG101|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36706192
Organism
Homo sapiens
Experiment description
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name
Sci Adv
Publication year
2023
Sample
Embryonic kidney cells
Sample name
HEK293T
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
28
Experiment ID
1280
MISEV standards
Biophysical techniques
✔
TSG101|CD9|FLOT1|FLOT2|RAB35|RAB5A|GAPDH|TFRC|CD63|Alix
Enriched markers
✔
PCNA|CANX|GAPDH|HSP90AA1|HSP90B1
Negative markers
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
36706192
Organism
Homo sapiens
Experiment description
Displaying and delivering viral membrane antigens via WW domain-activated extracellular vesicles
Authors
"Choi S, Yang Z, Wang Q, Qiao Z, Sun M, Wiggins J, Xiang SH, Lu Q."
Journal name
Sci Adv
Publication year
2023
Sample
Embryonic kidney cells
Sample name
HEK293T - Fraction 5
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
1.151 g/mL
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
29
Experiment ID
226
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|CD9|FLOT1
Enriched markers
✔
LMNA|H2AFX|ATP5A1|TOMM20
Negative markers
✘
Particle analysis
Identified molecule
mRNA
Identification method
Small RNA sequencing (Illumina HiSeq 2000 (Solexa)
PubMed ID
26027894
Organism
Homo sapiens
Experiment description
"Quantitative and qualitative analysis of small RNAs in human endothelial cells and exosomes provides insights into localized RNA processing, degradation and sorting"
Authors
"Bas W. M. van Balkom, Almut S. Eisele, D. Michiel Pegtel, Sander Bervoets, Marianne C. Verhaar"
Journal name
Journal of Extracellular Vesicles
Publication year
2015
Sample
Endothelial cells
Sample name
HMEC-1
Isolation/purification methods
Differential ultracentrifugation Sucrose density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
miRNA Protein snoRNA lncRNA yRNA snRNA mRNA ncRNA mtRNA vtRNA scaRNA lincRNA
Methods used in the study
Small RNA sequencing (Illumina HiSeq 2000 (Solexa) Western blotting
30
Experiment ID
405
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Foreskin fibroblasts
Sample name
BJ
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
31
Experiment ID
363
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
32
Experiment ID
364
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
33
Experiment ID
365
MISEV standards
✘
Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
33991177
Organism
Homo sapiens
Experiment description
Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors
"Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name
Proteomics
Publication year
2021
Sample
Gastric cancer cells
Sample name
MKN1 - 100K pellet
Isolation/purification methods
Differential centrifugation Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
34
Experiment ID
4
MISEV standards
✔
IEM
Biophysical techniques
✔
CD63|MHCI|MHCII
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
11487543
Organism
Homo sapiens
Experiment description
Intestinal epithelial cells secrete exosome-like vesicles.
Authors
"van Niel G, Raposo G, Candalh C, Boussac M, Hershberg R, Cerf-Bensussan N, Heyman M"
Journal name
Not applicable
Publication year
2001
Sample
Intestinal epithelial cells
Sample name
HT29-19A T84-DRB1*0401/CIITA
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [MALDI TOF] Western blotting
35
Experiment ID
417
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Lymphoma cells
Sample name
Raji
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
36
Experiment ID
411
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Mammary cancer-associated fibroblasts
Sample name
mCAF
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
37
Experiment ID
126
MISEV standards
✘
Biophysical techniques
✔
GAPDH
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry [LTQ-FT Ultra]
PubMed ID
Unpublished / Not applicable
Organism
Homo sapiens
Experiment description
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy?
Authors
"Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim"
Journal name
Mesenchymal Stem Cell Therapy
Publication year
2011
Sample
Mesenchymal stem cells
Sample name
huES9.E1
Isolation/purification methods
HPLC
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Antobody array Mass spectrometry
38
Experiment ID
418
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Monocytic leukemia cells
Sample name
THP-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
39
Experiment ID
224
MISEV standards
✔
EM|AFM
Biophysical techniques
✔
Alix|TSG101|CD63|CD81
Enriched markers
✔
GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25944692
Organism
Homo sapiens
Experiment description
Proteogenomic analysis reveals exosomes are more oncogenic than ectosomes
Authors
"Keerthikumar S, Gangoda L, Liem M, Fonseka P, Atukorala I, Ozcitti C, Mechler A, Adda CG, Ang CS, Mathivanan S"
Journal name
Oncotarget
Publication year
2015
Sample
Neuroblastoma cells
Sample name
SH-SY5Y
Isolation/purification methods
Differential centrifugation Ultracentrifugation OptiPrep density gradient
Flotation density
1.10 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Western blotting
40
Experiment ID
413
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Normal mammary epithelial cells
Sample name
MCF10A
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
41
Experiment ID
211
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
cytochrome c|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
IGROV1
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
42
Experiment ID
212
MISEV standards
✔
CEM
Biophysical techniques
✔
TSG101|Alix|EpCAM|TFRC
Enriched markers
✔
Cytochrome C|GOLGA2
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23333927
Organism
Homo sapiens
Experiment description
Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors
"Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K."
Journal name
J Proteomics
Publication year
2013
Sample
Ovarian cancer cells
Sample name
OVCAR-3
Isolation/purification methods
Differential centrifugation Ultracentrifugation Sucrose density gradient
Flotation density
1.09-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
43
Experiment ID
406
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35|CD81
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
BxPC3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
44
Experiment ID
415
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
45
Experiment ID
434
MISEV standards
✘
Biophysical techniques
✔
SDCBP|FLOT1|CD81|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 7-10 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Size exclusion chromatography
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
46
Experiment ID
435
MISEV standards
✘
Biophysical techniques
✔
CD81|SDCBP|FLOT1|CD9|CD151|FLOT2|TSG101|LAMP1|TFRC|RAB5A|RAB35|GAPDH|UCHL1|ICAM1
Enriched markers
✔
CANX
Negative markers
✘
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic cancer cells
Sample name
PANC-1 - Exo-rich fractions 1-6 pooled
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation OptiPrep density gradient centrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
47
Experiment ID
408
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD63|CD81|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX|ACTB
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPDE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
48
Experiment ID
409
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pancreatic duct epithalial cells
Sample name
HPNE
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
49
Experiment ID
231
MISEV standards
✘
Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 1
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein Lipids
Methods used in the study
Western blotting Mass spectrometry
50
Experiment ID
232
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 2
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
51
Experiment ID
233
MISEV standards
✘
Biophysical techniques
✘
Enriched markers
✘
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25332113
Organism
Homo sapiens
Experiment description
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors
"Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name
Transfusion
Publication year
2015
Sample
Platelets
Sample name
PL-Exs - Rep 3
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation Optiprep density gradient
Flotation density
1.12-1.15 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
52
Experiment ID
416
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD9|CD63|SDCBP|LAMP1|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
Pluripotent stem cells
Sample name
PSC
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
53
Experiment ID
138
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
"Hosseini-Beheshti E, Guns ES."
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
DU145 - Rep 2
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
54
Experiment ID
139
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
"Hosseini-Beheshti E, Guns ES."
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
DU145 - Rep 3
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
55
Experiment ID
140
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|RAB5|LAMP2|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
"Hosseini-Beheshti E, Guns ES."
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
VCaP - Rep 2
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
56
Experiment ID
146
MISEV standards
✔
EM
Biophysical techniques
✔
HSP70|HSP90|CD9
Enriched markers
✔
HSP90B1
Negative markers
✘
Particle analysis
Identified molecule
protein
Identification method
PubMed ID
22723089
Organism
Homo sapiens
Experiment description
Prostate cancer cell derived exosomes
Authors
"Hosseini-Beheshti E, Guns ES."
Journal name
MCP
Publication year
2012
Sample
Prostate cancer cells
Sample name
RWPE - Rep 2
Isolation/purification methods
Sucrose density gradient
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry [QTOF]
57
Experiment ID
275
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel sensitive
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.12-1.19 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry/Flow cytometry/Western blotting
58
Experiment ID
274
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|RAB5A|CD9|CD82|CD63|CD81
Enriched markers
✔
AIF
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
25844599
Organism
Homo sapiens
Experiment description
Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors
"Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T."
Journal name
Oncotarget
Publication year
2015
Sample
Prostate cancer cells
Sample name
DU145 - Docetaxel resistant
Isolation/purification methods
Filtration Ultracentrifugation Sucrose density gradient
Flotation density
1.13-1.18 g/mL
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry Flow cytometry Western blotting
59
Experiment ID
834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
60
Experiment ID
835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
35333565
Organism
Homo sapiens
Experiment description
LAMP2A regulates the loading of proteins into exosomes
Authors
"Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name
Sci Adv
Publication year
2022
Sample
Retinal pigment epithelial cells
Sample name
ARPE-19
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Western blotting Mass spectrometry
61
Experiment ID
410
MISEV standards
✔
EM
Biophysical techniques
✔
CD81|CD63|SDCBP|LAMP1|CD9|GAPDH|FLOT1|TFRC|FLOT2|TSG101|RAB35
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule
Protein
Identification method
Mass spectrometry
PubMed ID
34108659
Organism
Homo sapiens
Experiment description
Quantitative Proteomics Identifies the Core Proteome of Exosomes with Syntenin-1 as the highest abundant protein and a Putative Universal Biomarker
Authors
"Kugeratski FG, Hodge K, Lilla S, McAndrews KM, Zhou X, Hwang RF, Zanivan S, Kalluri R."
Journal name
Nat Cell Biol
Publication year
2021
Sample
T lymphocytes
Sample name
Jurkat
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein mRNA
Methods used in the study
RT-qPCR Western blotting Mass spectrometry Flow cytometry
62
Experiment ID
217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘
Negative markers
✔
NTA
Particle analysis
Identified molecule
protein
Identification method
Mass spectrometry
PubMed ID
23844026
Organism
Homo sapiens
Experiment description
Characterization of human thymic exosomes.
Authors
"Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name
PLoS One
Publication year
2013
Sample
Thymus
Sample name
Normal-Thymus
Isolation/purification methods
Differential centrifugation Filtration Ultracentrifugation
Flotation density
-
Molecules identified in the study
Protein
Methods used in the study
Mass spectrometry
Protein-protein interactions for TUBB4B
Protein Interactor
ExoCarta ID
Identification method
PubMed
Species
1
UBL4A
8266
Affinity Capture-MS
Homo sapiens
2
SHC1
6464
Affinity Capture-MS
Homo sapiens
3
RIN3
Affinity Capture-MS
Homo sapiens
4
DYNC1I2
1781
Affinity Capture-MS
Homo sapiens
5
CRK
1398
Affinity Capture-MS
Homo sapiens
6
TUBB8
347688
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
7
SETX
Affinity Capture-MS
Homo sapiens
8
VHL
Affinity Capture-MS
Homo sapiens
9
MID1
4281
Affinity Capture-MS
Homo sapiens
10
SCG2
Two-hybrid
Homo sapiens
11
TACC2
10579
Affinity Capture-MS
Homo sapiens
12
HMMR
Affinity Capture-MS
Homo sapiens
13
EFNA3
Affinity Capture-MS
Homo sapiens
14
WDR62
Affinity Capture-MS
Homo sapiens
15
WDR76
Affinity Capture-MS
Homo sapiens
16
ZBTB21
Affinity Capture-MS
Homo sapiens
17
GNS
2799
Co-fractionation
Homo sapiens
18
GLMP
112770
Affinity Capture-MS
Homo sapiens
19
PFDN4
5203
Affinity Capture-MS
Homo sapiens
20
POU5F1
Affinity Capture-MS
Homo sapiens
21
PPEF1
Affinity Capture-MS
Homo sapiens
22
MAGED2
10916
Affinity Capture-MS
Homo sapiens
23
HERC2
8924
Affinity Capture-MS
Homo sapiens
24
ARIH2
10425
Affinity Capture-MS
Homo sapiens
25
PFDN6
10471
Affinity Capture-MS
Homo sapiens
26
DYNLT1
6993
Affinity Capture-MS
Homo sapiens
27
KIF14
9928
Affinity Capture-MS
Homo sapiens
28
CLASP2
23122
Affinity Capture-MS
Homo sapiens
29
BAG5
9529
Affinity Capture-MS
Homo sapiens
30
AURKA
6790
Affinity Capture-MS
Homo sapiens
31
DNAL4
Affinity Capture-MS
Homo sapiens
32
USP11
8237
Affinity Capture-MS
Homo sapiens
33
MAP1B
4131
Co-fractionation
Homo sapiens
34
PSME1
5720
Two-hybrid
Homo sapiens
35
CDH1
999
Proximity Label-MS
Homo sapiens
36
YAP1
10413
Affinity Capture-MS
Homo sapiens
37
MYH9
4627
Co-fractionation
Homo sapiens
38
TRIM21
6737
Affinity Capture-MS
Homo sapiens
39
SLX4
Affinity Capture-MS
Homo sapiens
40
PEX14
5195
Co-purification
Homo sapiens
41
BICD2
23299
Affinity Capture-MS
Homo sapiens
42
YWHAQ
10971
Affinity Capture-MS
Homo sapiens
43
HECTD1
25831
Affinity Capture-MS
Homo sapiens
44
CUL2
8453
Affinity Capture-MS
Homo sapiens
45
TRMT2A
27037
Affinity Capture-MS
Homo sapiens
46
KCNH5
Affinity Capture-MS
Homo sapiens
47
CCNT1
Co-fractionation
Homo sapiens
48
ID2
Affinity Capture-MS
Homo sapiens
49
MGMT
4255
Affinity Capture-MS
Homo sapiens
50
ACD
Two-hybrid
Homo sapiens
51
PHLPP1
Proximity Label-MS
Homo sapiens
52
HADHA
3030
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
53
AHCYL1
10768
Co-fractionation
Homo sapiens
54
ZNF622
90441
Affinity Capture-MS
Homo sapiens
55
IQCB1
Affinity Capture-MS
Homo sapiens
56
MADD
8567
Affinity Capture-MS
Homo sapiens
57
FN1
2335
Affinity Capture-MS
Homo sapiens
58
TXNDC9
10190
Affinity Capture-MS
Homo sapiens
59
TUBA4A
7277
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
60
PCBP1
5093
Affinity Capture-MS
Homo sapiens
61
RPA3
6119
Affinity Capture-MS
Homo sapiens
62
DLST
1743
Two-hybrid
Homo sapiens
63
CDC20
991
Affinity Capture-MS
Homo sapiens
64
TCP11L2
Affinity Capture-MS
Homo sapiens
65
STMN1
3925
Affinity Capture-MS
Homo sapiens
66
TBCA
6902
Affinity Capture-MS
Homo sapiens
67
CTNNA1
1495
Affinity Capture-MS
Homo sapiens
68
PAFAH1B1
5048
Affinity Capture-MS
Homo sapiens
69
TUBGCP2
10844
Affinity Capture-MS
Homo sapiens
70
Tubg1
103733
Affinity Capture-MS
Mus musculus
71
CCT7
10574
Affinity Capture-MS
Homo sapiens
72
GRB2
2885
Affinity Capture-MS
Homo sapiens
73
ZEB1
Affinity Capture-MS
Homo sapiens
74
CENPJ
55835
Affinity Capture-MS
Homo sapiens
75
TUBGCP3
10426
Affinity Capture-MS
Homo sapiens
76
PFDN1
5201
Affinity Capture-MS
Homo sapiens
77
ZC2HC1A
51101
Affinity Capture-MS
Homo sapiens
78
DCTN2
10540
Affinity Capture-MS
Homo sapiens
79
HSP90AA1
3320
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
80
RPS15A
6210
Co-fractionation
Homo sapiens
81
SSBP1
6742
Affinity Capture-MS
Homo sapiens
82
PSMA1
5682
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
83
Tuba3a
22144
Affinity Capture-MS
Mus musculus
84
GPR50
Affinity Capture-MS
Homo sapiens
85
CNTRL
Affinity Capture-MS
Homo sapiens
86
CLASP1
23332
Affinity Capture-MS
Homo sapiens
87
CAPS
828
Affinity Capture-MS
Homo sapiens
88
CYLD
Affinity Capture-MS
Homo sapiens
89
STMN3
Affinity Capture-MS
Homo sapiens
90
TXNIP
10628
Affinity Capture-MS
Homo sapiens
91
SNW1
22938
Affinity Capture-MS
Homo sapiens
92
CLIP4
Affinity Capture-MS
Homo sapiens
93
CEP170
9859
Affinity Capture-MS
Homo sapiens
94
RANGAP1
5905
Affinity Capture-MS
Homo sapiens
95
TRAF1
7185
Affinity Capture-MS
Homo sapiens
96
PTPRF
5792
Two-hybrid
Homo sapiens
Affinity Capture-MS
Homo sapiens
97
SPRTN
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
98
CLIP1
6249
Affinity Capture-MS
Homo sapiens
99
EML2
24139
Affinity Capture-MS
Homo sapiens
100
RPA2
6118
Affinity Capture-MS
Homo sapiens
101
ENOX2
Two-hybrid
Homo sapiens
102
APC
Affinity Capture-MS
Homo sapiens
103
HSP90AB1
3326
Affinity Capture-MS
Homo sapiens
104
FDXR
2232
Affinity Capture-MS
Homo sapiens
105
LOC260334
Affinity Capture-MS
Homo sapiens
106
CAND1
55832
Affinity Capture-MS
Homo sapiens
107
AFTPH
Affinity Capture-MS
Homo sapiens
108
MFAP5
Affinity Capture-MS
Homo sapiens
109
DNAJB6
10049
Affinity Capture-MS
Homo sapiens
110
LGALS3BP
3959
Affinity Capture-MS
Homo sapiens
111
LRP1
4035
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
112
PIGN
23556
Affinity Capture-MS
Homo sapiens
113
B3GNT2
10678
Affinity Capture-MS
Homo sapiens
114
JAKMIP1
152789
Affinity Capture-MS
Homo sapiens
115
PSMA3
5684
Affinity Capture-MS
Homo sapiens
116
COPS6
10980
Affinity Capture-MS
Homo sapiens
117
FAM83D
81610
Affinity Capture-MS
Homo sapiens
118
PRC1
9055
Affinity Capture-MS
Homo sapiens
119
ACTR1A
10121
Affinity Capture-MS
Homo sapiens
120
RECQL4
Affinity Capture-MS
Homo sapiens
121
OBSL1
23363
Affinity Capture-MS
Homo sapiens
122
TINF2
Two-hybrid
Homo sapiens
123
STIP1
10963
Affinity Capture-MS
Homo sapiens
124
TUBA3C
7278
Co-fractionation
Homo sapiens
125
VCAM1
7412
Affinity Capture-MS
Homo sapiens
126
TUBB6
84617
Co-fractionation
Homo sapiens
127
CUL1
8454
Affinity Capture-MS
Homo sapiens
128
ZNHIT6
54680
Affinity Capture-MS
Homo sapiens
129
DLGAP5
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
130
DYNC1H1
1778
Affinity Capture-MS
Homo sapiens
131
YWHAB
7529
Co-fractionation
Homo sapiens
132
CCT2
10576
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
133
VRK3
51231
Affinity Capture-MS
Homo sapiens
134
HIST1H4A
8359
Affinity Capture-MS
Homo sapiens
135
TBCE
6905
Affinity Capture-MS
Homo sapiens
136
PSMD14
10213
Affinity Capture-MS
Homo sapiens
137
CBL
867
Affinity Capture-MS
Homo sapiens
138
CAPSL
Affinity Capture-MS
Homo sapiens
139
SNRNP200
23020
Co-fractionation
Homo sapiens
140
TUBB2B
347733
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
141
TUBA1C
84790
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
142
RLIM
51132
Affinity Capture-MS
Homo sapiens
143
ACTR10
55860
Affinity Capture-MS
Homo sapiens
144
Ksr1
Affinity Capture-MS
Mus musculus
145
RAD21
5885
Affinity Capture-Western
Homo sapiens
146
FBXL4
26235
Affinity Capture-MS
Homo sapiens
147
TUBB
203068
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Cross-Linking-MS (XL-MS)
Homo sapiens
Co-fractionation
Homo sapiens
148
MAP7
9053
Affinity Capture-MS
Homo sapiens
149
AIFM1
9131
Affinity Capture-MS
Homo sapiens
150
XPO1
7514
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
151
TPR
7175
Co-fractionation
Homo sapiens
152
IKZF3
Affinity Capture-MS
Homo sapiens
153
PRKAR2A
5576
Affinity Capture-MS
Homo sapiens
154
CDC42EP1
11135
Affinity Capture-MS
Homo sapiens
155
PRCP
5547
Co-fractionation
Homo sapiens
156
TCP11L1
55346
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
157
EGFR
1956
Affinity Capture-MS
Homo sapiens
158
HOOK3
84376
Affinity Capture-MS
Homo sapiens
159
IKZF1
Affinity Capture-MS
Homo sapiens
160
RIPK4
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
161
EML3
256364
Affinity Capture-MS
Homo sapiens
162
CDK2
1017
Affinity Capture-MS
Homo sapiens
163
CEP250
11190
Affinity Capture-MS
Homo sapiens
164
ZMYM6
Affinity Capture-MS
Homo sapiens
165
MYC
Affinity Capture-MS
Homo sapiens
166
IL12RB1
Affinity Capture-MS
Homo sapiens
167
CUL7
9820
Affinity Capture-MS
Homo sapiens
168
DDRGK1
65992
Affinity Capture-MS
Homo sapiens
169
Yap1
Reconstituted Complex
Mus musculus
170
ISOC1
51015
Co-fractionation
Homo sapiens
171
PCM1
5108
Affinity Capture-MS
Homo sapiens
172
PFDN5
5204
Affinity Capture-MS
Homo sapiens
173
HUWE1
10075
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
174
TACC3
Affinity Capture-MS
Homo sapiens
175
TAF6
6878
Co-fractionation
Homo sapiens
176
EIF3F
8665
Affinity Capture-MS
Homo sapiens
177
KIF21B
23046
Affinity Capture-MS
Homo sapiens
178
SPOP
Affinity Capture-MS
Homo sapiens
179
TTC5
Affinity Capture-MS
Homo sapiens
180
TP53
7157
Affinity Capture-MS
Homo sapiens
181
FOLR1
2348
Affinity Capture-MS
Homo sapiens
182
MELK
Affinity Capture-MS
Homo sapiens
183
PLD6
Affinity Capture-MS
Homo sapiens
184
CLIP2
7461
Affinity Capture-MS
Homo sapiens
185
GNL1
2794
Co-fractionation
Homo sapiens
186
PLD3
23646
Affinity Capture-MS
Homo sapiens
187
SPAG5
10615
Affinity Capture-MS
Homo sapiens
188
C16orf72
29035
Affinity Capture-MS
Homo sapiens
189
DYNLRB1
83658
Affinity Capture-MS
Homo sapiens
190
PRMT1
3276
Affinity Capture-MS
Homo sapiens
191
DYNC1LI1
51143
Affinity Capture-MS
Homo sapiens
192
RANBP2
5903
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
193
TCP1
6950
Affinity Capture-MS
Homo sapiens
194
ATP6V1B2
526
Co-fractionation
Homo sapiens
195
HNRNPC
3183
Co-fractionation
Homo sapiens
196
TRAPPC2
6399
Affinity Capture-MS
Homo sapiens
197
RAB1A
5861
Co-fractionation
Homo sapiens
198
ALMS1
Affinity Capture-MS
Homo sapiens
199
PFKP
5214
Co-fractionation
Homo sapiens
200
YWHAE
7531
Affinity Capture-MS
Homo sapiens
201
RPS19
6223
Co-fractionation
Homo sapiens
202
CAMSAP2
Affinity Capture-MS
Homo sapiens
203
RPS6KB2
Affinity Capture-MS
Homo sapiens
204
CEP97
79598
Affinity Capture-MS
Homo sapiens
205
CAPZB
832
Affinity Capture-MS
Homo sapiens
206
RPL10A
4736
Co-fractionation
Homo sapiens
207
RUVBL1
8607
Co-fractionation
Homo sapiens
208
TPSB2
Affinity Capture-MS
Homo sapiens
209
DYNC1LI2
1783
Affinity Capture-MS
Homo sapiens
210
MCM2
4171
Affinity Capture-MS
Homo sapiens
211
CAMSAP1
157922
Affinity Capture-MS
Homo sapiens
212
COPB2
9276
Co-fractionation
Homo sapiens
213
MCTS1
28985
Co-fractionation
Homo sapiens
214
BAGE2
Affinity Capture-MS
Homo sapiens
215
PLD2
5338
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
216
MID2
11043
Affinity Capture-MS
Homo sapiens
217
ITGA4
3676
Affinity Capture-MS
Homo sapiens
218
CKAP5
9793
Affinity Capture-MS
Homo sapiens
219
RC3H2
Affinity Capture-MS
Homo sapiens
220
TUBGCP4
27229
Affinity Capture-MS
Homo sapiens
221
TFCP2
7024
Affinity Capture-MS
Homo sapiens
222
CEP131
22994
Affinity Capture-MS
Homo sapiens
223
GPRIN1
114787
Affinity Capture-MS
Homo sapiens
224
HSPA8
3312
Affinity Capture-MS
Homo sapiens
225
FAS
355
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
226
KLK5
25818
Affinity Capture-MS
Homo sapiens
227
BSG
682
Affinity Capture-MS
Homo sapiens
228
FOXC1
Affinity Capture-MS
Homo sapiens
229
EML4
27436
Affinity Capture-MS
Homo sapiens
230
SOCS6
Affinity Capture-MS
Homo sapiens
231
CUL4B
8450
Affinity Capture-MS
Homo sapiens
232
PARK2
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
233
B4GALT2
Affinity Capture-MS
Homo sapiens
234
TUBA1A
7846
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
235
ASNA1
439
Co-fractionation
Homo sapiens
236
PDCD5
9141
Affinity Capture-MS
Homo sapiens
237
SLAIN1
122060
Affinity Capture-MS
Homo sapiens
238
TNPO2
30000
Co-fractionation
Homo sapiens
239
BTF3
689
Affinity Capture-MS
Homo sapiens
240
NTRK1
4914
Affinity Capture-MS
Homo sapiens
241
U2AF2
11338
Co-fractionation
Homo sapiens
242
DNAJA1
3301
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
243
TACC1
6867
Affinity Capture-MS
Homo sapiens
244
KPNB1
3837
Affinity Capture-MS
Homo sapiens
245
TUBA1B
10376
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
246
Dlgap4
Affinity Capture-MS
Mus musculus
247
HDAC5
10014
Affinity Capture-MS
Homo sapiens
248
Nek9
Affinity Capture-MS
Mus musculus
249
POLR2B
5431
Co-fractionation
Homo sapiens
Co-fractionation
Homo sapiens
250
TTL
150465
Affinity Capture-MS
Homo sapiens
251
OGT
8473
Reconstituted Complex
Homo sapiens
252
MYCBP2
23077
Affinity Capture-MS
Homo sapiens
253
METTL14
Affinity Capture-MS
Homo sapiens
254
SMARCA5
8467
Co-fractionation
Homo sapiens
255
DIRAS2
54769
Affinity Capture-MS
Homo sapiens
256
MGARP
Affinity Capture-MS
Homo sapiens
257
NR3C1
2908
Affinity Capture-MS
Homo sapiens
258
ACE2
59272
Affinity Capture-MS
Homo sapiens
259
MAP1LC3A
84557
Affinity Capture-MS
Homo sapiens
260
ARL2
402
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
261
VBP1
7411
Affinity Capture-MS
Homo sapiens
262
AP2M1
1173
Affinity Capture-MS
Homo sapiens
263
CEP170B
Affinity Capture-MS
Homo sapiens
264
NLRP1
Affinity Capture-MS
Homo sapiens
265
JUNB
Co-fractionation
Homo sapiens
266
RNF2
Affinity Capture-MS
Homo sapiens
267
KIAA0368
23392
Affinity Capture-MS
Homo sapiens
268
HSPD1
3329
Co-fractionation
Homo sapiens
269
NEUROG3
Affinity Capture-MS
Homo sapiens
270
MPHOSPH9
Affinity Capture-MS
Homo sapiens
271
TUBB2A
7280
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
272
KIF2A
3796
Affinity Capture-MS
Homo sapiens
273
PHB2
11331
Co-fractionation
Homo sapiens
274
CCT8
10694
Affinity Capture-MS
Homo sapiens
275
CDC42SE2
56990
Affinity Capture-MS
Homo sapiens
276
TUBA4B
80086
Co-fractionation
Homo sapiens
277
MCM5
4174
Affinity Capture-MS
Homo sapiens
278
EPHA4
2043
Affinity Capture-MS
Homo sapiens
279
RPA1
6117
Affinity Capture-MS
Homo sapiens
280
NME2P1
283458
Affinity Capture-MS
Homo sapiens
281
TUBB3
10381
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
282
AICDA
Affinity Capture-Western
Homo sapiens
283
PGAM2
5224
Affinity Capture-MS
Homo sapiens
284
ZC2HC1B
Affinity Capture-MS
Homo sapiens
285
UFL1
23376
Affinity Capture-MS
Homo sapiens
286
CDK13
8621
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
287
HDAC6
10013
Affinity Capture-MS
Homo sapiens
288
RPL8
6132
Co-fractionation
Homo sapiens
289
METTL3
Affinity Capture-MS
Homo sapiens
290
SERBP1
26135
Affinity Capture-MS
Homo sapiens
291
ATXN3
4287
Affinity Capture-MS
Homo sapiens
292
KIF4A
24137
Affinity Capture-MS
Homo sapiens
293
CDKN2A
1029
Reconstituted Complex
Homo sapiens
294
UBE2H
7328
Affinity Capture-MS
Homo sapiens
295
INSIG1
Affinity Capture-MS
Homo sapiens
296
SLFN11
91607
Affinity Capture-MS
Homo sapiens
297
CCT3
7203
Affinity Capture-MS
Homo sapiens
298
POT1
Two-hybrid
Homo sapiens
299
SSR2
Affinity Capture-MS
Homo sapiens
300
SMURF1
57154
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
301
NCALD
83988
Affinity Capture-MS
Homo sapiens
302
FBXO25
Affinity Capture-MS
Homo sapiens
303
WNT7A
Affinity Capture-MS
Homo sapiens
304
CCT4
10575
Affinity Capture-MS
Homo sapiens
305
ZBTB1
Affinity Capture-MS
Homo sapiens
306
POTEF
728378
Affinity Capture-MS
Homo sapiens
307
USP36
Affinity Capture-MS
Homo sapiens
308
ASB17
Affinity Capture-MS
Homo sapiens
309
PPP1R12A
4659
Co-fractionation
Homo sapiens
310
DCTN4
51164
Affinity Capture-MS
Homo sapiens
311
COPS5
10987
Affinity Capture-MS
Homo sapiens
312
AGO3
Affinity Capture-MS
Homo sapiens
313
ALK
238
Affinity Capture-MS
Homo sapiens
314
NFXL1
152518
Affinity Capture-MS
Homo sapiens
315
MYCN
Affinity Capture-MS
Homo sapiens
316
HAUS4
Affinity Capture-MS
Homo sapiens
317
STMN2
Affinity Capture-MS
Homo sapiens
318
TUBA3D
113457
Co-fractionation
Homo sapiens
319
HAUS2
Affinity Capture-MS
Homo sapiens
320
EMD
2010
Affinity Capture-MS
Homo sapiens
321
HSP90B1
7184
Affinity Capture-MS
Homo sapiens
322
CCT5
22948
Affinity Capture-MS
Homo sapiens
323
CPVL
54504
Affinity Capture-MS
Homo sapiens
324
GCC2
9648
Affinity Capture-MS
Homo sapiens
325
PFDN2
5202
Affinity Capture-MS
Homo sapiens
326
VCP
7415
Affinity Capture-MS
Homo sapiens
Co-fractionation
Homo sapiens
Affinity Capture-MS
Homo sapiens
327
SUMO2
6613
Reconstituted Complex
Homo sapiens
328
DCTN1
1639
Affinity Capture-MS
Homo sapiens
329
BTK
695
Affinity Capture-MS
Homo sapiens
330
CEP76
Affinity Capture-MS
Homo sapiens
331
CDK9
1025
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
332
EFNB3
1949
Affinity Capture-MS
Homo sapiens
333
H2AFY
9555
Co-fractionation
Homo sapiens
334
CEBPA
Protein-peptide
Homo sapiens
335
SSX2IP
Affinity Capture-MS
Homo sapiens
336
DNAJC7
7266
Affinity Capture-MS
Homo sapiens
337
ZMYM4
Affinity Capture-MS
Homo sapiens
338
NEURL4
Affinity Capture-MS
Homo sapiens
339
DLEU1
Two-hybrid
Homo sapiens
340
GAN
8139
Affinity Capture-MS
Homo sapiens
341
GFER
Affinity Capture-MS
Homo sapiens
342
FANCD2
Affinity Capture-MS
Homo sapiens
343
CUL3
8452
Affinity Capture-MS
Homo sapiens
344
RPL23
9349
Affinity Capture-MS
Homo sapiens
345
OTUD1
220213
Affinity Capture-MS
Homo sapiens
346
LETM1
3954
Affinity Capture-MS
Homo sapiens
347
CCT6A
908
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
348
CAMSAP3
Affinity Capture-MS
Homo sapiens
349
CTNNB1
1499
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
350
ERCC8
Affinity Capture-MS
Homo sapiens
351
CCP110
Affinity Capture-MS
Homo sapiens
352
VARS
7407
Co-fractionation
Homo sapiens
353
RPL7
6129
Co-fractionation
Homo sapiens
354
TBCD
6904
Affinity Capture-MS
Homo sapiens
Affinity Capture-MS
Homo sapiens
355
ADAM22
Affinity Capture-MS
Homo sapiens
356
AKAP8
Co-fractionation
Homo sapiens
357
MAP7D1
55700
Affinity Capture-MS
Homo sapiens
358
AGO4
Affinity Capture-MS
Homo sapiens
359
SLAIN2
Affinity Capture-MS
Homo sapiens
360
DCUN1D1
54165
Affinity Capture-MS
Homo sapiens
361
NIN
51199
Affinity Capture-MS
Homo sapiens
362
TXNL1
9352
Co-fractionation
Homo sapiens
363
NUP214
8021
Affinity Capture-MS
Homo sapiens
364
TMPRSS5
Affinity Capture-MS
Homo sapiens
365
ZMYM2
Affinity Capture-MS
Homo sapiens
366
ARRB2
409
Affinity Capture-MS
Homo sapiens
367
TUBG1
7283
Affinity Capture-MS
Homo sapiens
368
INPPL1
3636
Affinity Capture-MS
Homo sapiens
369
DNAJA2
10294
Co-fractionation
Homo sapiens
370
DSTYK
25778
Affinity Capture-MS
Homo sapiens
371
OSTM1
28962
Affinity Capture-MS
Homo sapiens
372
CUL5
8065
Affinity Capture-MS
Homo sapiens
373
LMAN1
3998
Affinity Capture-MS
Homo sapiens
374
GBA
2629
Affinity Capture-MS
Homo sapiens
375
EPB41L2
2037
Co-fractionation
Homo sapiens
376
TUBB1
81027
Affinity Capture-MS
Homo sapiens
377
TUBB4A
10382
Affinity Capture-MS
Homo sapiens
378
KIAA1429
25962
Affinity Capture-MS
Homo sapiens
379
TERF1
7013
Two-hybrid
Homo sapiens
380
EZH2
Affinity Capture-MS
Homo sapiens
381
SLC25A4
291
Co-fractionation
Homo sapiens
382
CAD
790
Co-fractionation
Homo sapiens
383
AIRE
Affinity Capture-MS
Homo sapiens
384
TRIM31
Affinity Capture-MS
Homo sapiens
385
PINK1
Affinity Capture-MS
Homo sapiens
386
CUL4A
8451
Affinity Capture-MS
Homo sapiens
387
C9orf72
Affinity Capture-MS
Homo sapiens
388
NEDD8
4738
Affinity Capture-MS
Homo sapiens
Reconstituted Complex
Homo sapiens
389
CDC5L
988
Affinity Capture-MS
Homo sapiens
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Pathways in which TUBB4B is involved