Gene description for TUBGCP4
Gene name tubulin, gamma complex associated protein 4
Gene symbol TUBGCP4
Other names/aliases 76P
GCP-4
GCP4
Grip76
MCCRP3
Species Homo sapiens
 Database cross references - TUBGCP4
ExoCarta ExoCarta_27229
Vesiclepedia VP_27229
Entrez Gene 27229
HGNC 16691
MIM 609610
UniProt Q9UGJ1  
 TUBGCP4 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for TUBGCP4
Molecular Function
    structural constituent of cytoskeleton GO:0005200 NAS
    protein binding GO:0005515 IPI
    gamma-tubulin binding GO:0043015 IBA
    microtubule minus-end binding GO:0051011 IBA
Biological Process
    mitotic cell cycle GO:0000278 IBA
    microtubule nucleation GO:0007020 IBA
    microtubule nucleation GO:0007020 NAS
    cytoplasmic microtubule organization GO:0031122 IBA
    spindle assembly GO:0051225 IBA
    meiotic cell cycle GO:0051321 IBA
    protein-containing complex assembly GO:0065003 NAS
Subcellular Localization
    spindle pole GO:0000922 IEA
    gamma-tubulin complex GO:0000930 IBA
    gamma-tubulin ring complex GO:0000931 NAS
    centrosome GO:0005813 IDA
    cytosol GO:0005829 TAS
    microtubule GO:0005874 IEA
    microtubule cytoskeleton GO:0015630 TAS
    membrane GO:0016020 HDA
    recycling endosome GO:0055037 IDA
 Experiment description of studies that identified TUBGCP4 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for TUBGCP4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 FDXACB1  
Two-hybrid Homo sapiens
2 RIT1 6016
Negative Genetic Homo sapiens
3 HSD17B11 51170
Affinity Capture-MS Homo sapiens
4 AP2B1 163
Two-hybrid Homo sapiens
5 Mzt1  
Affinity Capture-MS Mus musculus
6 KRT6B 3854
Two-hybrid Homo sapiens
7 RPS3A 6189
Affinity Capture-MS Homo sapiens
8 CD68 968
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 WWP1 11059
Affinity Capture-MS Homo sapiens
10 CMTM8  
Affinity Capture-MS Homo sapiens
11 HCST  
Affinity Capture-MS Homo sapiens
12 GLYCTK  
Two-hybrid Homo sapiens
13 SPERT  
Two-hybrid Homo sapiens
14 PDCD1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 UBA1 7317
Affinity Capture-MS Homo sapiens
16 RPS19 6223
Affinity Capture-MS Homo sapiens
17 C9orf78 51759
Affinity Capture-MS Homo sapiens
18 EFTUD1 79631
Affinity Capture-MS Homo sapiens
19 TMEM9B 56674
Affinity Capture-MS Homo sapiens
20 RPL14 9045
Affinity Capture-MS Homo sapiens
21 PYCR1 5831
Affinity Capture-MS Homo sapiens
22 TRIM55  
Two-hybrid Homo sapiens
23 TXNDC12 51060
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
24 MRPS14  
Affinity Capture-MS Homo sapiens
25 Tubgcp6  
Affinity Capture-MS Mus musculus
26 ATP5L 10632
Affinity Capture-MS Homo sapiens
27 POLR3K  
Affinity Capture-MS Homo sapiens
28 LGALS3BP 3959
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 SLC18A2  
Affinity Capture-MS Homo sapiens
30 NME7 29922
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
31 MRPS23 51649
Affinity Capture-MS Homo sapiens
32 NUDT3 11165
Two-hybrid Homo sapiens
33 OPRM1 4988
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
34 MRPS24 64951
Affinity Capture-MS Homo sapiens
35 Mzt2  
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
36 POU5F1  
Affinity Capture-MS Homo sapiens
37 SIGMAR1 10280
Affinity Capture-MS Homo sapiens
38 Tubgcp3  
Affinity Capture-MS Mus musculus
39 USHBP1  
Two-hybrid Homo sapiens
40 MZT2B 80097
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 TUBGCP6 85378
Affinity Capture-MS Homo sapiens
42 PZP 5858
Affinity Capture-MS Homo sapiens
43 MRPS7 51081
Affinity Capture-MS Homo sapiens
44 BTRC 8945
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 UQCC2  
Affinity Capture-MS Homo sapiens
46 GPR17 2840
Affinity Capture-MS Homo sapiens
47 KIF14 9928
Affinity Capture-MS Homo sapiens
48 MZT1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 HSPA8 3312
Affinity Capture-MS Homo sapiens
50 NINL  
Affinity Capture-Western Homo sapiens
51 HSPA9 3313
Affinity Capture-MS Homo sapiens
52 VSIG1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 EMD 2010
Affinity Capture-MS Homo sapiens
54 SLC5A6 8884
Affinity Capture-MS Homo sapiens
55 COPE 11316
Affinity Capture-MS Homo sapiens
56 TUBA1A 7846
Affinity Capture-MS Homo sapiens
57 GOLGA2 2801
Two-hybrid Homo sapiens
58 RNF146  
Two-hybrid Homo sapiens
59 RPL18A 6142
Affinity Capture-MS Homo sapiens
60 TUBA1C 84790
Affinity Capture-MS Homo sapiens
61 AMPD2 271
Affinity Capture-MS Homo sapiens
62 C16orf58 64755
Affinity Capture-MS Homo sapiens
63 RPL38 6169
Affinity Capture-MS Homo sapiens
64 IGF2BP1 10642
Affinity Capture-MS Homo sapiens
65 GFAP 2670
Two-hybrid Homo sapiens
66 GPR182  
Affinity Capture-MS Homo sapiens
67 C5orf42  
Affinity Capture-MS Homo sapiens
68 MOGS 7841
Affinity Capture-MS Homo sapiens
69 SCAF11  
Affinity Capture-MS Homo sapiens
70 MZT2A  
Affinity Capture-MS Homo sapiens
71 LZTR1 8216
Two-hybrid Homo sapiens
72 PAK7  
Two-hybrid Homo sapiens
73 ACTG1 71
Affinity Capture-MS Homo sapiens
74 SLC2A9  
Affinity Capture-MS Homo sapiens
75 TRIM63  
Two-hybrid Homo sapiens
76 MRPL41 64975
Affinity Capture-MS Homo sapiens
77 MRPL28 10573
Affinity Capture-MS Homo sapiens
78 HSPA5 3309
Affinity Capture-MS Homo sapiens
79 SLC22A6 9356
Affinity Capture-MS Homo sapiens
80 TUBB 203068
Affinity Capture-MS Homo sapiens
81 SPTLC1 10558
Affinity Capture-MS Homo sapiens
82 APLNR  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
83 TUBB2A 7280
Affinity Capture-MS Homo sapiens
84 RPLP2 6181
Affinity Capture-MS Homo sapiens
85 TUBGCP5 114791
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
86 RPL13 6137
Affinity Capture-MS Homo sapiens
87 FPR1  
Affinity Capture-MS Homo sapiens
88 HIGD1A 25994
Affinity Capture-MS Homo sapiens
89 SDSL 113675
Two-hybrid Homo sapiens
90 MAD1L1  
Two-hybrid Homo sapiens
91 CD2BP2 10421
Affinity Capture-MS Homo sapiens
92 TUBB4B 10383
Affinity Capture-MS Homo sapiens
93 CMKLR1 1240
Affinity Capture-MS Homo sapiens
94 CUL3 8452
Affinity Capture-MS Homo sapiens
95 ATP5F1 515
Affinity Capture-MS Homo sapiens
96 RPL21 6144
Affinity Capture-MS Homo sapiens
97 RPL3 6122
Affinity Capture-MS Homo sapiens
98 EGFR 1956
Negative Genetic Homo sapiens
99 KRT4 3851
Two-hybrid Homo sapiens
100 OBFC1 79991
Two-hybrid Homo sapiens
101 OPRL1 4987
Affinity Capture-MS Homo sapiens
102 RPL7 6129
Affinity Capture-MS Homo sapiens
103 RPS27 6232
Affinity Capture-MS Homo sapiens
104 KRT15 3866
Two-hybrid Homo sapiens
105 ATP5J2 9551
Affinity Capture-MS Homo sapiens
106 RPA3 6119
Proximity Label-MS Homo sapiens
107 GPR45  
Affinity Capture-MS Homo sapiens
108 WWC1  
Affinity Capture-MS Homo sapiens
109 CCDC85B  
Two-hybrid Homo sapiens
110 ZNF785  
Affinity Capture-MS Homo sapiens
111 DGCR6  
Two-hybrid Homo sapiens
112 HSPA1A 3303
Affinity Capture-MS Homo sapiens
113 CHD8 57680
Affinity Capture-MS Homo sapiens
114 P2RY10  
Affinity Capture-MS Homo sapiens
115 Nedd1  
Affinity Capture-MS Mus musculus
116 FBF1  
Two-hybrid Homo sapiens
117 MRPL38  
Affinity Capture-MS Homo sapiens
118 RPS16 6217
Affinity Capture-MS Homo sapiens
119 AVPR2  
Affinity Capture-MS Homo sapiens
120 SYNRG 11276
Affinity Capture-MS Homo sapiens
121 PRICKLE3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
122 KRT18 3875
Two-hybrid Homo sapiens
123 RBM14 10432
Affinity Capture-MS Homo sapiens
124 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
125 FBXW11  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
126 Tubgcp5  
Affinity Capture-MS Mus musculus
127 TUBGCP2 10844
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
128 Tubg1 103733
Affinity Capture-MS Mus musculus
Affinity Capture-MS Mus musculus
129 TMEM9 252839
Affinity Capture-MS Homo sapiens
130 RPS3 6188
Affinity Capture-MS Homo sapiens
131 TOR1AIP2 163590
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
132 TUBG1 7283
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Co-fractionation Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
133 TUBG2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
134 YBEY  
Affinity Capture-MS Homo sapiens
135 OPALIN  
Affinity Capture-MS Homo sapiens
136 TUBGCP3 10426
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
137 CHRM4  
Affinity Capture-MS Homo sapiens
138 RPS26P50  
Affinity Capture-MS Homo sapiens
139 UBB 7314
Affinity Capture-MS Homo sapiens
140 MRPS18B 28973
Affinity Capture-MS Homo sapiens
141 NEDD1 121441
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
142 MGME1  
Affinity Capture-MS Homo sapiens
143 RPS8 6202
Affinity Capture-MS Homo sapiens
144 RPL27 6155
Affinity Capture-MS Homo sapiens
145 RPRD1B 58490
Two-hybrid Homo sapiens
146 KCTD17 79734
Two-hybrid Homo sapiens
147 MAS1  
Affinity Capture-MS Homo sapiens
148 KRAS 3845
Negative Genetic Homo sapiens
149 HNRNPU 3192
Affinity Capture-MS Homo sapiens
150 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
151 VIM 7431
Two-hybrid Homo sapiens
152 ALDH1L2 160428
Affinity Capture-MS Homo sapiens
153 FPR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
154 CDC5L 988
Affinity Capture-MS Homo sapiens
155 ADGRG2 10149
Affinity Capture-MS Homo sapiens
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