Gene description for SH3KBP1
Gene name SH3-domain kinase binding protein 1
Gene symbol SH3KBP1
Other names/aliases CD2BP3
CIN85
GIG10
HSB-1
HSB1
MIG18
Species Homo sapiens
 Database cross references - SH3KBP1
ExoCarta ExoCarta_30011
Entrez Gene 30011
HGNC 13867
MIM 300374
UniProt Q96B97  
 SH3KBP1 identified in exosomes derived from the following tissue/cell type
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Prostate cancer cells 25844599    
Prostate cancer cells 25844599    
 Gene ontology annotations for SH3KBP1
Molecular Function
    protein binding GO:0005515 IPI
    SH3 domain binding GO:0017124 IEA
Biological Process
    endocytosis GO:0006897 IEA
    cytoskeleton organization GO:0007010 IMP
    cell migration GO:0016477 IMP
    regulation of cell shape GO:0008360 IMP
    negative regulation of epidermal growth factor receptor signaling pathway GO:0042059 TAS
    cell-cell signaling GO:0007267 NAS
    apoptotic process GO:0006915 IEA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
Subcellular Localization
    cytoskeleton GO:0005856 IEA
    focal adhesion GO:0005925 IEA
    synapse GO:0045202 IEA
    cytosol GO:0005829 TAS
    neuron projection GO:0043005 IEA
    cell-cell junction GO:0005911 IEA
    plasma membrane GO:0005886 TAS
    cytoplasmic vesicle membrane GO:0030659 IEA
 Experiment description of studies that identified SH3KBP1 in exosomes
1
Experiment ID 207
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 208
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 209
ISEV standards
✘ EV Biophysical techniques
✔
TSG101|HSP70
EV Cytosolic markers
✔
FLOT1
EV Membrane markers
✔
VDAC
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ.
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 275
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel sensitive
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.12-1.19 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry/Flow cytometry/Western blotting
5
Experiment ID 274
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|RAB5A
EV Cytosolic markers
✔
CD9|CD82|CD63|CD81
EV Membrane markers
✔
AIF
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25844599    
Organism Homo sapiens
Experiment description Molecular profiling of prostate cancer derived exosomes may reveal a predictive signature for response to docetaxel.
Authors Kharaziha P, Chioureas D, Rutishauser D, Baltatzis G, Lennartsson L, Fonseca P, Azimi A, Hultenby K, Zubarev R, Ullen A, Yachnin J, Nilsson S, Panaretakis T.
Journal name Oncotarget
Publication year 2015
Sample Prostate cancer cells
Sample name DU145 - Docetaxel resistant
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.18 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
Flow cytometry
Western blotting
 Protein-protein interactions for SH3KBP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 SHKBP1  
Invitro Homo sapiens
Invivo Homo sapiens
2 TAF6 6878
Affinity Capture-Western Homo sapiens
3 SYNJ1  
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
4 NEDD4 4734
Invivo Homo sapiens
Two-hybrid Homo sapiens
5 GRB2 2885
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invivo Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
6 ARHGAP27  
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
7 SH3GL1 6455
Affinity Capture-MS Homo sapiens
8 CRK 1398
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
9 BCAR1 9564
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
10 PAK2 5062
Affinity Capture-Western Homo sapiens
Far Western Homo sapiens
11 PDCD6 10016
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
12 CBLB  
Invivo Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
13 CLYBL  
Affinity Capture-Western Homo sapiens
14 PDGFRB 5159
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
15 SH3GLB2  
Reconstituted Complex Homo sapiens
16 CBL 867
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
17 SH3GL3  
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
18 SH3GL2  
Invitro Homo sapiens
19 ABI2 10152
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
20 TJP2 9414
Affinity Capture-Western Homo sapiens
21 SH3GLB1 51100
Reconstituted Complex Homo sapiens
22 PIK3CA 5290
Invivo Homo sapiens
Invitro Homo sapiens
23 CD2 914
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
24 SH3KBP1 30011
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
25 BLNK  
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
26 SOS1  
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
27 PIK3R1 5295
Invivo Homo sapiens
28 MET 4233
Affinity Capture-Western Homo sapiens
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