Gene description for DAB2
Gene name Dab, mitogen-responsive phosphoprotein, homolog 2 (Drosophila)
Gene symbol DAB2
Other names/aliases DOC-2
DOC2
Species Homo sapiens
 Database cross references - DAB2
ExoCarta ExoCarta_1601
Vesiclepedia VP_1601
Entrez Gene 1601
HGNC 2662
MIM 601236
UniProt P98082  
 DAB2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for DAB2
Molecular Function
    protein C-terminus binding GO:0008022 IPI
    cargo receptor activity GO:0038024 IMP
    protein binding GO:0005515 IPI
    integrin binding GO:0005178 IEA
    clathrin adaptor activity GO:0035615 IMP
    AP-2 adaptor complex binding GO:0035612 IEA
    SMAD binding GO:0046332 IDA
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IEA
Biological Process
    Wnt signaling pathway GO:0016055 IEA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IMP
    integrin-mediated signaling pathway GO:0007229 IMP
    negative regulation of protein localization to plasma membrane GO:1903077 IMP
    negative regulation of apoptotic process GO:0043066 IDA
    negative regulation of extrinsic apoptotic signaling pathway GO:2001237 IEA
    positive regulation of cell migration GO:0030335 IMP
    pinocytosis GO:0006907 IEA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IMP
    clathrin coat assembly GO:0048268 IEA
    positive regulation of Wnt signaling pathway, planar cell polarity pathway GO:2000096 IMP
    cell morphogenesis involved in differentiation GO:0000904 IEA
    positive regulation of early endosome to late endosome transport GO:2000643 IMP
    positive regulation of SMAD protein import into nucleus GO:0060391 IDA
    positive regulation of transcription, DNA-templated GO:0045893 IMP
    apoptotic process GO:0006915 IEA
    positive regulation of epithelial to mesenchymal transition GO:0010718 IDA
    negative regulation of transcription, DNA-templated GO:0045892 IMP
    positive regulation of clathrin-mediated endocytosis GO:2000370 IMP
    cellular response to transforming growth factor beta stimulus GO:0071560 IEA
    positive regulation of transcription elongation from RNA polymerase II promoter GO:0032968 IEA
    negative regulation of androgen receptor signaling pathway GO:0060766 IMP
    excretion GO:0007588 IEA
    positive regulation of integrin-mediated signaling pathway GO:2001046 IEA
    cell proliferation GO:0008283 TAS
    receptor-mediated endocytosis GO:0006898 IEA
    negative regulation of protein binding GO:0032091 IMP
    positive regulation of endocytosis GO:0045807 IMP
    activation of JUN kinase activity GO:0007257 IEA
    positive regulation of receptor recycling GO:0001921 IEA
    protein transport GO:0015031 IEA
    in utero embryonic development GO:0001701 IEA
    leading edge cell differentiation GO:0035026 IMP
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IEA
    positive regulation of pathway-restricted SMAD protein phosphorylation GO:0010862 IDA
    positive regulation of protein phosphorylation GO:0001934 IMP
    myeloid cell differentiation GO:0030099 IEA
    membrane organization GO:0061024 TAS
    positive regulation of transforming growth factor beta receptor signaling pathway GO:0030511 IDA
    endoderm development GO:0007492 IEA
Subcellular Localization
    focal adhesion GO:0005925 IDA
    apical plasma membrane GO:0016324 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    transforming growth factor beta receptor homodimeric complex GO:0070022 IDA
    extracellular exosome GO:0070062 IDA
    lysosomal membrane GO:0005765 TAS
    nucleolus GO:0005730 IDA
    coated pit GO:0005905 IDA
    clathrin-coated vesicle GO:0030136 IDA
    plasma membrane GO:0005886 TAS
    clathrin coat of coated pit GO:0030132 IEA
    clathrin-coated vesicle membrane GO:0030665 IEA
 Experiment description of studies that identified DAB2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 231
MISEV standards
✘ Biophysical techniques
✔
Alix|CD63|CD9
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ Biophysical techniques
✘ Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for DAB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 APP 351
Reconstituted Complex Homo sapiens
2 APLP1 333
Reconstituted Complex Homo sapiens
3 FGR 2268
Invitro Homo sapiens
4 DVL1 1855
Reconstituted Complex Homo sapiens
5 GRB2 2885
Affinity Capture-MS Homo sapiens
6 DVL2 1856
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
7 CSK 1445
Invivo Homo sapiens
Invitro Homo sapiens
8 AXIN1  
Reconstituted Complex Homo sapiens
9 PIN1 5300
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
10 LRP2 4036
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
11 DAB2IP 153090
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
12 DIP2A 23181
Affinity Capture-MS Homo sapiens
13 DVL3 1857
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
14 CDK1 983
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
15 AP2M1 1173
Affinity Capture-MS Homo sapiens
16 TGFBR1 7046
Affinity Capture-Western Homo sapiens
17 LRP1 4035
Reconstituted Complex Homo sapiens
18 APLP2 334
Reconstituted Complex Homo sapiens
19 MYO6 4646
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
20 LDLR 3949
Reconstituted Complex Homo sapiens
21 SMAD3 4088
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
22 AP2A2 161
Affinity Capture-Western Homo sapiens
23 SMAD2 4087
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
24 SRC 6714
Invivo Homo sapiens
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
25 INPP5D 3635
Reconstituted Complex Homo sapiens
26 TGFBR2 7048
Affinity Capture-Western Homo sapiens
View the network image/svg+xml
 Pathways in which DAB2 is involved
PathwayEvidenceSource
Formation of annular gap junctions TAS Reactome
Gap junction degradation TAS Reactome





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