Gene description for DAB2
Gene name Dab, mitogen-responsive phosphoprotein, homolog 2 (Drosophila)
Gene symbol DAB2
Other names/aliases DOC-2
DOC2
Species Homo sapiens
 Database cross references - DAB2
ExoCarta ExoCarta_1601
Vesiclepedia VP_1601
Entrez Gene 1601
HGNC 2662
MIM 601236
UniProt P98082  
 DAB2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for DAB2
Molecular Function
    protein binding GO:0005515 IPI
    clathrin adaptor activity GO:0035615 IBA
    clathrin adaptor activity GO:0035615 IMP
    cargo receptor activity GO:0038024 IBA
    cargo receptor activity GO:0038024 IMP
    SMAD binding GO:0046332 IDA
    low-density lipoprotein particle receptor binding GO:0050750 IEA
Biological Process
    negative regulation of transcription by RNA polymerase II GO:0000122 IMP
    positive regulation of protein phosphorylation GO:0001934 IMP
    receptor-mediated endocytosis GO:0006898 IBA
    apoptotic process GO:0006915 IEA
    transforming growth factor beta receptor signaling pathway GO:0007179 IDA
    integrin-mediated signaling pathway GO:0007229 IMP
    positive regulation of epithelial to mesenchymal transition GO:0010718 IBA
    positive regulation of epithelial to mesenchymal transition GO:0010718 IDA
    negative regulation of neuron projection development GO:0010977 IEA
    protein transport GO:0015031 IEA
    Wnt signaling pathway GO:0016055 IEA
    negative regulation of cell growth GO:0030308 IEA
    positive regulation of cell migration GO:0030335 IMP
    negative regulation of protein binding GO:0032091 IMP
    positive regulation of aldosterone biosynthetic process GO:0032349 IEA
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 IMP
    leading edge cell differentiation GO:0035026 IMP
    negative regulation of apoptotic process GO:0043066 IDA
    positive regulation of endocytosis GO:0045807 IBA
    positive regulation of endocytosis GO:0045807 IMP
    positive regulation of transcription by RNA polymerase II GO:0045944 IMP
    clathrin coat assembly GO:0048268 IEA
    response to steroid hormone GO:0048545 IEA
    negative regulation of epithelial cell proliferation GO:0050680 IEA
    positive regulation of SMAD protein signal transduction GO:0060391 IDA
    negative regulation of androgen receptor signaling pathway GO:0060766 IMP
    negative regulation of ERK1 and ERK2 cascade GO:0070373 IEA
    cellular response to epidermal growth factor stimulus GO:0071364 IEA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IBA
    negative regulation of canonical Wnt signaling pathway GO:0090090 IMP
    negative regulation of canonical Wnt signaling pathway GO:0090090 ISS
    positive regulation of substrate adhesion-dependent cell spreading GO:1900026 IEA
    response to salt GO:1902074 IEA
    negative regulation of protein localization to plasma membrane GO:1903077 IMP
    positive regulation of Wnt signaling pathway, planar cell polarity pathway GO:2000096 IMP
    positive regulation of clathrin-dependent endocytosis GO:2000370 IMP
    positive regulation of early endosome to late endosome transport GO:2000643 IMP
    positive regulation of aldosterone secretion GO:2000860 IEA
Subcellular Localization
    fibrillar center GO:0001650 IDA
    cytoplasm GO:0005737 IBA
    lysosomal membrane GO:0005765 TAS
    cytosol GO:0005829 TAS
    plasma membrane GO:0005886 IDA
    plasma membrane GO:0005886 TAS
    clathrin-coated pit GO:0005905 IBA
    clathrin-coated pit GO:0005905 IDA
    focal adhesion GO:0005925 HDA
    clathrin-coated vesicle GO:0030136 IDA
    clathrin-coated vesicle membrane GO:0030665 IEA
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
 Experiment description of studies that identified DAB2 in exosomes
1
Experiment ID 489
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
EM
Biophysical techniques
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
Canx
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
9
Experiment ID 834
MISEV standards
EM
Biophysical techniques
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
10
Experiment ID 835
MISEV standards
EM
Biophysical techniques
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for DAB2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ACTR2 10097
Affinity Capture-MS Homo sapiens
2 APLP1 333
Reconstituted Complex Homo sapiens
3 ISG15 9636
Affinity Capture-MS Homo sapiens
4 AP2B1 163
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 PPP1CB 5500
Affinity Capture-MS Homo sapiens
6 SERPINB4 6318
Affinity Capture-MS Homo sapiens
7 Tpm1 22003
Affinity Capture-MS Mus musculus
8 CSK 1445
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
9 ATG9A 79065
Proximity Label-MS Homo sapiens
10 ACOT9 23597
Affinity Capture-MS Homo sapiens
11 Calml3  
Affinity Capture-MS Mus musculus
12 DBN1 1627
Affinity Capture-MS Homo sapiens
13 Myo6  
Reconstituted Complex Rattus norvegicus
14 PYCR1 5831
Affinity Capture-MS Homo sapiens
15 DVL3 1857
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
16 Actb 11461
Affinity Capture-MS Mus musculus
17 CLTB 1212
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
18 APP 351
Reconstituted Complex Homo sapiens
19 TGFBR1 7046
Affinity Capture-Western Homo sapiens
20 APEX1 328
Affinity Capture-RNA Homo sapiens
21 LRP1 4035
Reconstituted Complex Homo sapiens
22 CDC16 8881
Two-hybrid Homo sapiens
23 AP2A1 160
Affinity Capture-MS Homo sapiens
24 IQGAP1 8826
Affinity Capture-MS Homo sapiens
25 CPSF7 79869
Affinity Capture-MS Homo sapiens
26 SRC 6714
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
27 HSPH1 10808
Affinity Capture-MS Homo sapiens
28 STX6 10228
Proximity Label-MS Homo sapiens
29 GTSE1 51512
Affinity Capture-MS Homo sapiens
30 Sec24c  
Affinity Capture-MS Mus musculus
31 DVL2 1856
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
32 LAMTOR1 55004
Proximity Label-MS Homo sapiens
33 NUP155 9631
Affinity Capture-MS Homo sapiens
34 TFAP2A  
Affinity Capture-Western Homo sapiens
35 AURKA 6790
Affinity Capture-MS Homo sapiens
36 MYO18A 399687
Affinity Capture-MS Homo sapiens
37 MAPRE1 22919
Affinity Capture-MS Homo sapiens
38 MCAM 4162
Proximity Label-MS Homo sapiens
39 APLP2 334
Reconstituted Complex Homo sapiens
40 MYH9 4627
Affinity Capture-MS Homo sapiens
41 SLC43A3 29015
Affinity Capture-MS Homo sapiens
42 SMAD3 4088
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
43 DTX2 113878
Proximity Label-MS Homo sapiens
44 SMAD2 4087
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
45 BMP2K 55589
Affinity Capture-MS Homo sapiens
46 MALT1 10892
Affinity Capture-MS Homo sapiens
47 ACTB 60
Affinity Capture-MS Homo sapiens
48 PICALM 8301
Affinity Capture-MS Homo sapiens
49 GAK 2580
Affinity Capture-MS Homo sapiens
50 CCNT1  
Affinity Capture-MS Homo sapiens
51 LRP8 7804
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
52 ANLN 54443
Affinity Capture-MS Homo sapiens
53 LIMA1 51474
Affinity Capture-MS Homo sapiens
54 SF3B2 10992
Affinity Capture-MS Homo sapiens
55 AHCY 191
Affinity Capture-MS Homo sapiens
56 Myh9 17886
Affinity Capture-MS Mus musculus
57 XPO1 7514
Affinity Capture-MS Homo sapiens
58 CLTA 1211
Proximity Label-MS Homo sapiens
59 TRIO 7204
Affinity Capture-MS Homo sapiens
60 DAB2IP 153090
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
61 YLPM1 56252
Affinity Capture-MS Homo sapiens
62 MYO19  
Affinity Capture-MS Homo sapiens
63 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 FN1 2335
Affinity Capture-MS Homo sapiens
65 AP2M1 1173
Affinity Capture-MS Homo sapiens
66 DCAF6 55827
Affinity Capture-MS Homo sapiens
67 SYNPO 11346
Affinity Capture-MS Homo sapiens
68 MYO6 4646
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Co-localization Homo sapiens
FRET Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
69 FCHO2 115548
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 AP2A2 161
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
71 CLTC 1213
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
72 INPP5D 3635
Reconstituted Complex Homo sapiens
73 Ppp1cb 19046
Affinity Capture-MS Mus musculus
74 TGFBR2 7048
Affinity Capture-Western Homo sapiens
75 SEC24B 10427
Affinity Capture-MS Homo sapiens
76 Lima1  
Affinity Capture-MS Mus musculus
77 FGR 2268
Reconstituted Complex Homo sapiens
78 DVL1 1855
Reconstituted Complex Homo sapiens
79 Tmod3 50875
Affinity Capture-MS Mus musculus
80 Tfap2a  
Reconstituted Complex Rattus norvegicus
81 KIF14 9928
Affinity Capture-MS Homo sapiens
82 ITSN1 6453
Two-hybrid Homo sapiens
83 GRB2 2885
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
84 CPSF6 11052
Affinity Capture-MS Homo sapiens
85 ATXN1L  
Two-hybrid Homo sapiens
86 PIN1 5300
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
87 SEC23B 10483
Affinity Capture-MS Homo sapiens
88 Myo1c 17913
Affinity Capture-MS Mus musculus
89 CAPZA2 830
Affinity Capture-MS Homo sapiens
90 AXIN1  
Reconstituted Complex Homo sapiens
91 CDK1 983
Affinity Capture-Western Homo sapiens
Biochemical Activity Homo sapiens
92 Myh10 77579
Affinity Capture-MS Mus musculus
93 Fcho2  
Affinity Capture-MS Mus musculus
94 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
95 HNRNPL 3191
Affinity Capture-MS Homo sapiens
96 REPS1 85021
Affinity Capture-MS Homo sapiens
97 FLNA 2316
Affinity Capture-MS Homo sapiens
98 LRP2 4036
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
99 PRDX1 5052
Affinity Capture-MS Homo sapiens
100 C14orf166 51637
Affinity Capture-MS Homo sapiens
101 LDLR 3949
Reconstituted Complex Homo sapiens
102 Flnb 286940
Affinity Capture-MS Mus musculus
103 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
104 SMURF2 64750
Two-hybrid Homo sapiens
105 CFTR 1080
Affinity Capture-Western Homo sapiens
106 HSPA1B 3304
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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