Gene description for GAK
Gene name cyclin G associated kinase
Gene symbol GAK
Other names/aliases DNAJ26
DNAJC26
Species Homo sapiens
 Database cross references - GAK
ExoCarta ExoCarta_2580
Vesiclepedia VP_2580
Entrez Gene 2580
HGNC 4113
MIM 602052
UniProt O14976  
 GAK identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Colorectal cancer cells 23161513    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for GAK
Molecular Function
    protein serine/threonine kinase activity GO:0004674 TAS
    3-phosphoinositide-dependent protein kinase activity GO:0004676 IEA
    DNA-dependent protein kinase activity GO:0004677 IEA
    AMP-activated protein kinase activity GO:0004679 IEA
    eukaryotic translation initiation factor 2alpha kinase activity GO:0004694 IEA
    ribosomal protein S6 kinase activity GO:0004711 IEA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    clathrin binding GO:0030276 IBA
    cyclin binding GO:0030332 ISS
    histone H3S10 kinase activity GO:0035175 IEA
    histone H3T11 kinase activity GO:0035402 IEA
    histone H3T6 kinase activity GO:0035403 IEA
    histone H2AXS139 kinase activity GO:0035979 IEA
    histone H3S28 kinase activity GO:0044022 IEA
    histone H4S1 kinase activity GO:0044023 IEA
    histone H2AS1 kinase activity GO:0044024 IEA
    histone H2BS14 kinase activity GO:0044025 IEA
    protein-folding chaperone binding GO:0051087 TAS
    histone H3T3 kinase activity GO:0072354 IEA
    histone H2AS121 kinase activity GO:0072371 IEA
    Rho-dependent protein serine/threonine kinase activity GO:0072518 IEA
    protein serine kinase activity GO:0106310 IEA
    histone H2BS36 kinase activity GO:0140823 IEA
    histone H3S57 kinase activity GO:0140855 IEA
    histone H3T45 kinase activity GO:0140857 IEA
    histone H2AT120 kinase activity GO:1990244 IEA
Biological Process
    chromatin remodeling GO:0006338 IEA
    receptor-mediated endocytosis GO:0006898 IMP
    endoplasmic reticulum organization GO:0007029 ISS
    Golgi organization GO:0007030 IMP
    negative regulation of neuron projection development GO:0010977 IDA
    synaptic vesicle uncoating GO:0016191 ISS
    protein localization to Golgi apparatus GO:0034067 IMP
    intracellular transport GO:0046907 IMP
    clathrin coat assembly GO:0048268 IMP
    chaperone cofactor-dependent protein refolding GO:0051085 TAS
    clathrin coat disassembly GO:0072318 IBA
    clathrin coat disassembly GO:0072318 IMP
    clathrin-dependent endocytosis GO:0072583 IBA
    clathrin-dependent endocytosis GO:0072583 IMP
    protein localization to plasma membrane GO:0072659 IMP
    Golgi to lysosome transport GO:0090160 IMP
    regulation of clathrin coat assembly GO:1905443 IMP
Subcellular Localization
    cytoplasm GO:0005737 IBA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 TAS
    focal adhesion GO:0005925 IEA
    membrane GO:0016020 HDA
    clathrin-coated vesicle GO:0030136 IDA
    vesicle GO:0031982 IBA
    vesicle GO:0031982 IDA
    intracellular membrane-bounded organelle GO:0043231 IBA
    intracellular membrane-bounded organelle GO:0043231 IDA
    perinuclear region of cytoplasm GO:0048471 IEA
    presynapse GO:0098793 IEA
 Experiment description of studies that identified GAK in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 207
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DKO-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
6
Experiment ID 208
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name Dks-8
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
7
Experiment ID 209
MISEV standards
✘ Biophysical techniques
✔
TSG101|HSP70|FLOT1
Enriched markers
✔
VDAC
Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23161513    
Organism Homo sapiens
Experiment description Proteomic analysis of exosomes from mutant KRAS colon cancer cells identifies intercellular transfer of mutant KRAS.
Authors "Demory Beckler M, Higginbotham JN, Franklin JL, Ham AJ, Halvey PJ, Imasuen IE, Whitwell C, Li M, Liebler DC, Coffey RJ."
Journal name Mol Cell Proteomics
Publication year 2012
Sample Colorectal cancer cells
Sample name DLD-1
Isolation/purification methods Differential centrifugation
Filtration
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
9
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for GAK
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 C5AR2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 AP2B1 163
Affinity Capture-MS Homo sapiens
3 LGR4 55366
Affinity Capture-MS Homo sapiens
4 CIB2  
Affinity Capture-MS Homo sapiens
5 ATG9A 79065
Proximity Label-MS Homo sapiens
6 WNK1 65125
Affinity Capture-MS Homo sapiens
7 ATP13A2  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
8 SP1  
Affinity Capture-MS Homo sapiens
9 DBN1 1627
Affinity Capture-MS Homo sapiens
10 LIMA1 51474
Affinity Capture-MS Homo sapiens
11 EPS15 2060
Affinity Capture-MS Homo sapiens
12 VHL  
Negative Genetic Homo sapiens
13 CLTB 1212
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 APP 351
Reconstituted Complex Homo sapiens
15 CAPZB 832
Affinity Capture-MS Homo sapiens
16 S100A4 6275
Affinity Capture-MS Homo sapiens
17 Eef1a1 13627
Affinity Capture-MS Mus musculus
18 TNK1  
Affinity Capture-MS Homo sapiens
19 USO1 8615
Two-hybrid Homo sapiens
20 IARS2 55699
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 MPP5 64398
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
22 ACTR3 10096
Proximity Label-MS Homo sapiens
23 AP2A1 160
Affinity Capture-MS Homo sapiens
24 FAM174A 345757
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 SEC24C 9632
Affinity Capture-MS Homo sapiens
26 FBXW7  
Synthetic Lethality Homo sapiens
27 IMPDH2 3615
Proximity Label-MS Homo sapiens
28 CPD 1362
Proximity Label-MS Homo sapiens
29 CPSF7 79869
Affinity Capture-MS Homo sapiens
30 SLC31A1 1317
Affinity Capture-MS Homo sapiens
31 AXIN1  
Two-hybrid Homo sapiens
32 CLINT1 9685
Affinity Capture-MS Homo sapiens
33 CEP162  
Affinity Capture-MS Homo sapiens
34 ALK 238
Affinity Capture-MS Homo sapiens
35 STX6 10228
Proximity Label-MS Homo sapiens
36 SRP68 6730
Affinity Capture-MS Homo sapiens
37 CHMP4A 29082
Affinity Capture-MS Homo sapiens
38 GTSE1 51512
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
39 AAK1 22848
Affinity Capture-MS Homo sapiens
40 RBM42  
Affinity Capture-MS Homo sapiens
41 NPAS1  
Affinity Capture-MS Homo sapiens
42 EBAG9 9166
Proximity Label-MS Homo sapiens
43 KIF3A 11127
Affinity Capture-MS Homo sapiens
44 RBM14 10432
Affinity Capture-MS Homo sapiens
45 LAMTOR1 55004
Proximity Label-MS Homo sapiens
46 METTL10  
Affinity Capture-MS Homo sapiens
47 HSPA8 3312
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
48 KIDINS220 57498
Affinity Capture-MS Homo sapiens
49 RB1 5925
Negative Genetic Homo sapiens
50 BAG5 9529
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
51 CPM 1368
Affinity Capture-MS Homo sapiens
52 FAM96A  
Affinity Capture-MS Homo sapiens
53 MRE11A 4361
Affinity Capture-MS Homo sapiens
54 CDK5 1020
Affinity Capture-Western Homo sapiens
55 Sec24c  
Affinity Capture-MS Mus musculus
56 CLTCL1 8218
Affinity Capture-MS Homo sapiens
57 RAB4A 5867
Proximity Label-MS Homo sapiens
58 RPL5 6125
Affinity Capture-MS Homo sapiens
59 NUMB 8650
Affinity Capture-MS Homo sapiens
60 NTRK1 4914
Affinity Capture-MS Homo sapiens
61 PARP1 142
Affinity Capture-MS Homo sapiens
62 PEX14 5195
Proximity Label-MS Homo sapiens
63 BMP2K 55589
Affinity Capture-MS Homo sapiens
64 PARP10 84875
Affinity Capture-MS Homo sapiens
65 PLXNA3 55558
Affinity Capture-MS Homo sapiens
66 PIK3C2A 5286
Affinity Capture-MS Homo sapiens
67 STON2 85439
Affinity Capture-MS Homo sapiens
68 STAMBP 10617
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
69 PICALM 8301
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
70 ATG7 10533
Affinity Capture-MS Homo sapiens
71 UBXN6 80700
Affinity Capture-MS Homo sapiens
72 Ppp2r1a 51792
Affinity Capture-MS Mus musculus
73 PSG11  
Affinity Capture-MS Homo sapiens
74 CALML3 810
Affinity Capture-MS Homo sapiens
75 PPM1B 5495
Co-fractionation Homo sapiens
76 Pafah1b1 18472
Affinity Capture-MS Mus musculus
77 WDR11 55717
Proximity Label-MS Homo sapiens
78 FAM83B  
Affinity Capture-MS Homo sapiens
79 PPM1M  
Affinity Capture-MS Homo sapiens
80 AHCY 191
Affinity Capture-MS Homo sapiens
81 BAG1 573
Affinity Capture-MS Homo sapiens
82 BAG2 9532
Affinity Capture-MS Homo sapiens
83 SEC13 6396
Affinity Capture-MS Homo sapiens
84 ZNF106  
Affinity Capture-MS Homo sapiens
85 SCAMP1 9522
Proximity Label-MS Homo sapiens
86 RAB29 8934
Affinity Capture-Western Homo sapiens
87 DAPP1 27071
Affinity Capture-MS Homo sapiens
88 NIPAL1  
Affinity Capture-MS Homo sapiens
89 PHKG2 5261
Affinity Capture-MS Homo sapiens
90 METTL21B  
Affinity Capture-MS Homo sapiens
91 HIP1R 9026
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
92 CALM1 801
Affinity Capture-MS Homo sapiens
93 Cep152  
Affinity Capture-MS Mus musculus
94 AP2M1 1173
Affinity Capture-MS Homo sapiens
95 PUS7L  
Affinity Capture-MS Homo sapiens
96 ACTRT1  
Affinity Capture-MS Homo sapiens
97 SYNPO 11346
Affinity Capture-MS Homo sapiens
98 DAB2 1601
Affinity Capture-MS Homo sapiens
99 MYO6 4646
Affinity Capture-MS Homo sapiens
100 RPA3 6119
Proximity Label-MS Homo sapiens
101 SEC16A 9919
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
102 AP2A2 161
Affinity Capture-MS Homo sapiens
103 CLASRP  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
104 HSPA1A 3303
Affinity Capture-MS Homo sapiens
105 TGFA  
Affinity Capture-MS Homo sapiens
106 HIP1 3092
Affinity Capture-MS Homo sapiens
107 HYOU1 10525
Proximity Label-MS Homo sapiens
108 PRKY  
Affinity Capture-MS Homo sapiens
109 CCNG1  
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
110 CLTC 1213
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
111 STAMBPL1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
112 DNAJC6  
Affinity Capture-MS Homo sapiens
Negative Genetic Homo sapiens
113 PHGDH 26227
Affinity Capture-MS Homo sapiens
114 GOLGB1 2804
Affinity Capture-MS Homo sapiens
115 CBLC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
116 MYO1C 4641
Affinity Capture-MS Homo sapiens
117 TNK2 10188
Affinity Capture-MS Homo sapiens
118 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
119 SPTAN1 6709
Affinity Capture-MS Homo sapiens
120 LGI1  
Affinity Capture-MS Homo sapiens
121 DENND1A 57706
Affinity Capture-MS Homo sapiens
122 DNAJA2 10294
Affinity Capture-MS Homo sapiens
123 MISP 126353
Affinity Capture-MS Homo sapiens
124 XPR1 9213
Affinity Capture-MS Homo sapiens
125 CDC37 11140
Affinity Capture-MS Homo sapiens
126 HNRNPA2B1 3181
Affinity Capture-MS Homo sapiens
127 DMWD  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
128 ARHGAP18 93663
Affinity Capture-MS Homo sapiens
129 FAM83H 286077
Affinity Capture-MS Homo sapiens
130 FEM1B 10116
Affinity Capture-MS Homo sapiens
131 DDX58 23586
Affinity Capture-RNA Homo sapiens
132 ANAPC2 29882
Proximity Label-MS Homo sapiens
133 C10orf88  
Affinity Capture-MS Homo sapiens
134 RABGEF1 27342
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
135 AFAP1 60312
Affinity Capture-MS Homo sapiens
136 PSMA5 5686
Affinity Capture-MS Homo sapiens
137 INF2 64423
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
138 LRRK2 120892
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Affinity Capture-Western Homo sapiens
139 CLTA 1211
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
140 HSPBP1 23640
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
141 GNB2L1 10399
Affinity Capture-MS Homo sapiens
142 GPX7 2882
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
143 Uso1 56041
Affinity Capture-MS Mus musculus
144 EDRF1  
Affinity Capture-MS Homo sapiens
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