Gene description for HIP1
Gene name huntingtin interacting protein 1
Gene symbol HIP1
Other names/aliases HIP-I
ILWEQ
SHON
Species Homo sapiens
 Database cross references - HIP1
ExoCarta ExoCarta_3092
Vesiclepedia VP_3092
Entrez Gene 3092
HGNC 4913
MIM 601767
UniProt O00291  
 HIP1 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Hepatocytes 26054723    
Mesenchymal stem cells 36408942    
 Gene ontology annotations for HIP1
Molecular Function
    epidermal growth factor receptor binding GO:0005154 TAS
    structural constituent of cytoskeleton GO:0005200 TAS
    protein binding GO:0005515 IPI
    phosphatidylinositol-4,5-bisphosphate binding GO:0005546 IDA
    clathrin binding GO:0030276 IDA
    clathrin light chain binding GO:0032051 IBA
    clathrin light chain binding GO:0032051 IPI
    phosphatidylinositol-3-phosphate binding GO:0032266 IDA
    phosphatidylinositol binding GO:0035091 IDA
    glutamate receptor binding GO:0035254 TAS
    AP-2 adaptor complex binding GO:0035612 IPI
    clathrin adaptor activity GO:0035615 IBA
    clathrin adaptor activity GO:0035615 IC
    protein homodimerization activity GO:0042803 IPI
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IBA
    phosphatidylinositol-3,4-bisphosphate binding GO:0043325 IDA
    protein heterodimerization activity GO:0046982 IPI
    actin filament binding GO:0051015 IBA
    actin filament binding GO:0051015 IDA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IBA
    phosphatidylinositol-3,5-bisphosphate binding GO:0080025 IDA
Biological Process
    endocytosis GO:0006897 IBA
    apoptotic process GO:0006915 IDA
    actin filament organization GO:0007015 IBA
    regulation of endocytosis GO:0030100 NAS
    cell differentiation GO:0030154 IEA
    regulation of apoptotic process GO:0042981 IBA
    regulation of apoptotic process GO:0042981 IDA
    positive regulation of epidermal growth factor receptor signaling pathway GO:0045742 IBA
    positive regulation of epidermal growth factor receptor signaling pathway GO:0045742 IMP
    positive regulation of receptor-mediated endocytosis GO:0048260 IMP
    clathrin coat assembly GO:0048268 IBA
    clathrin coat assembly GO:0048268 IDA
    clathrin coat assembly GO:0048268 IDA
    protein stabilization GO:0050821 IDA
    positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction GO:0051897 IC
    apoptotic signaling pathway GO:0097190 IDA
    regulation of postsynaptic neurotransmitter receptor internalization GO:0099149 IEA
    presynaptic modulation of chemical synaptic transmission GO:0099171 IEA
    neurotransmitter receptor transport GO:0099637 TAS
    positive regulation of platelet-derived growth factor receptor-beta signaling pathway GO:2000588 IBA
    positive regulation of platelet-derived growth factor receptor-beta signaling pathway GO:2000588 IMP
    positive regulation of cysteine-type endopeptidase activity GO:2001056 IDA
Subcellular Localization
    nucleus GO:0005634 TAS
    cytoplasm GO:0005737 IDA
    Golgi apparatus GO:0005794 IDA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 TAS
    membrane GO:0016020 TAS
    clathrin-coated vesicle GO:0030136 IBA
    clathrin-coated vesicle GO:0030136 IDA
    clathrin-coated vesicle membrane GO:0030665 IEA
    cortical actin cytoskeleton GO:0030864 IBA
    presynaptic membrane GO:0042734 IEA
    intracellular membrane-bounded organelle GO:0043231 IDA
    postsynaptic membrane GO:0045211 IEA
    Schaffer collateral - CA1 synapse GO:0098685 IEA
    presynapse GO:0098793 IBA
    presynapse GO:0098793 IDA
    postsynapse GO:0098794 TAS
    glutamatergic synapse GO:0098978 IEA
 Experiment description of studies that identified HIP1 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 237
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocytes
Sample name MIHA
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RNA Sequencing
6
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HIP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 GTSE1 51512
Affinity Capture-MS Homo sapiens
2 GJD3  
Proximity Label-MS Homo sapiens
3 PFDN1 5201
Two-hybrid Homo sapiens
4 PLEKHA4 57664
Affinity Capture-MS Homo sapiens
5 AP2B1 163
Proximity Label-MS Homo sapiens
6 SERBP1 26135
Affinity Capture-MS Homo sapiens
7 PARP1 142
Affinity Capture-MS Homo sapiens
8 ITSN1 6453
Two-hybrid Homo sapiens
9 CFAP53  
Two-hybrid Homo sapiens
10 BFAR  
Affinity Capture-Western Homo sapiens
11 ATG9A 79065
Proximity Label-MS Homo sapiens
12 EIF6 3692
Two-hybrid Homo sapiens
13 Hip1r  
Two-hybrid Mus musculus
14 REL 5966
Two-hybrid Homo sapiens
15 Calml3  
Affinity Capture-MS Mus musculus
16 EHHADH 1962
Two-hybrid Homo sapiens
17 RAD23B 5887
Two-hybrid Homo sapiens
18 CPNE2 221184
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
19 GLS2 27165
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
20 CASP8 841
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
21 ZFP1  
Affinity Capture-MS Homo sapiens
22 LITAF 9516
Two-hybrid Homo sapiens
23 SMURF1 57154
Biochemical Activity Homo sapiens
24 DAZAP2  
Two-hybrid Homo sapiens
25 EGFR 1956
Affinity Capture-Western Homo sapiens
26 ARHGAP18 93663
Affinity Capture-MS Homo sapiens
27 MAP3K1 4214
Biochemical Activity Homo sapiens
28 HIST1H2BD 3017
Affinity Capture-MS Homo sapiens
29 CLTB 1212
Reconstituted Complex Homo sapiens
Proximity Label-MS Homo sapiens
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
Co-crystal Structure Homo sapiens
30 HIP1R 9026
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-MS Homo sapiens
31 Ap2b1 140670
Two-hybrid Rattus norvegicus
32 CLTB 281698
Two-hybrid Bos taurus
33 HTT 3064
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
34 SYT17  
Two-hybrid Homo sapiens
35 CAPZB 832
Affinity Capture-MS Homo sapiens
36 MAPK6  
Two-hybrid Homo sapiens
37 C10orf88  
Affinity Capture-MS Homo sapiens
38 B3GAT1  
Proximity Label-MS Homo sapiens
39 UBASH3A 53347
Affinity Capture-MS Homo sapiens
40 MYH9 4627
Affinity Capture-MS Homo sapiens
41 CASP1  
Co-localization Homo sapiens
42 RPRM  
Two-hybrid Homo sapiens
43 NTRK1 4914
Affinity Capture-MS Homo sapiens
44 CLTA 1211
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 AP2A1 160
Reconstituted Complex Homo sapiens
46 KXD1 79036
Affinity Capture-MS Homo sapiens
47 DNAJC6  
Affinity Capture-MS Homo sapiens
48 RPS10 6204
Two-hybrid Homo sapiens
49 HIP1 3092
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
50 FBXO25  
Biochemical Activity Homo sapiens
51 CALM3 808
Affinity Capture-MS Homo sapiens
52 RHOB 388
Proximity Label-MS Homo sapiens
53 CLTC 1213
Affinity Capture-MS Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Co-localization Homo sapiens
Reconstituted Complex Homo sapiens
54 TCEANC  
Two-hybrid Homo sapiens
55 CCDC67  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
56 HNRNPA2B1 3181
Affinity Capture-MS Homo sapiens
57 FAM49B 51571
Two-hybrid Homo sapiens
58 IFT57 55081
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
59 TCEA2  
Two-hybrid Homo sapiens
60 GAK 2580
Affinity Capture-MS Homo sapiens
61 DIRAS3  
Proximity Label-MS Homo sapiens
62 STX6 10228
Proximity Label-MS Homo sapiens
63 PLCD3 113026
Affinity Capture-MS Homo sapiens
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