|
 Gene description for ARHGAP18 |
| Gene name |
Rho GTPase activating protein 18 |
| Gene symbol |
ARHGAP18 |
| Other names/aliases |
MacGAP
SENEX
bA307O14.2 |
| Species |
Homo sapiens |
| Database cross references - ARHGAP18 |
| ExoCarta |
ExoCarta_93663 |
| Vesiclepedia |
VP_93663 |
| Entrez Gene |
93663 |
| HGNC |
21035 |
| MIM |
613351 |
| UniProt |
Q8N392
|
| ARHGAP18 identified in exosomes derived from the following tissue/cell type |
|
Mesenchymal stem cells
|
Unpublished / Not applicable
|
|
Mesenchymal stem cells
|
36408942
|
|
Platelets
|
25332113
|
|
Platelets
|
25332113
|
|
Platelets
|
25332113
|
| Gene ontology annotations for ARHGAP18 |
|
|
| Experiment description of studies that identified ARHGAP18 in exosomes |
| 1 |
| Experiment ID |
126 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
GAPDH
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry [LTQ-FT Ultra]
|
| PubMed ID |
Unpublished / Not applicable
|
| Organism |
Homo sapiens |
| Experiment description |
Mesenchymal Stem Cell Exosomes: The Future MSC-based Therapy? |
| Authors |
Ruenn Chai Lai, Ronne Wee Yeh Yeo, Soon Sim Tan, Bin Zhang, Yijun Yin, Newman Siu Kwan Sze, Andre Choo, and Sai Kiang Lim |
| Journal name |
Mesenchymal Stem Cell Therapy
|
| Publication year |
2011 |
| Sample |
Mesenchymal stem cells |
| Sample name |
huES9.E1 |
| Isolation/purification methods |
HPLC |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Antobody array
Mass spectrometry |
|
|
| 2 |
| Experiment ID |
488 |
| MISEV standards |
|
✔
EM
|
EV Biophysical techniques |
|
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
|
EV Enriched markers |
|
✔
CANX
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
Protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
36408942
|
| Organism |
Homo sapiens |
| Experiment description |
Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells |
| Authors |
Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ. |
| Journal name |
Proteomics
|
| Publication year |
2023 |
| Sample |
Mesenchymal stem cells |
| Sample name |
UCMSC |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation |
| Flotation density |
-
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Western blotting
Mass spectometry |
|
|
| 3 |
| Experiment ID |
231 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✔
Alix|CD63|CD9
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✔
NTA
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25332113
|
| Organism |
Homo sapiens |
| Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
| Authors |
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. |
| Journal name |
Transfusion
|
| Publication year |
2015 |
| Sample |
Platelets |
| Sample name |
PL-Exs - Rep 1 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient |
| Flotation density |
1.12-1.15 g/mL
|
| Molecules identified in the study |
Protein
Lipids |
| Methods used in the study |
Western blotting
Mass spectrometry |
|
|
| 4 |
| Experiment ID |
232 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✘
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25332113
|
| Organism |
Homo sapiens |
| Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
| Authors |
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. |
| Journal name |
Transfusion
|
| Publication year |
2015 |
| Sample |
Platelets |
| Sample name |
PL-Exs - Rep 2 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient |
| Flotation density |
1.12-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| 5 |
| Experiment ID |
233 |
| MISEV standards |
|
✘
|
EV Biophysical techniques |
|
✘
|
EV Enriched markers |
|
✘
|
EV Negative markers |
|
✘
|
EV Particle analysis
|
|
| Identified molecule |
protein
|
| Identification method |
Mass spectrometry
|
| PubMed ID |
25332113
|
| Organism |
Homo sapiens |
| Experiment description |
Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets |
| Authors |
Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G. |
| Journal name |
Transfusion
|
| Publication year |
2015 |
| Sample |
Platelets |
| Sample name |
PL-Exs - Rep 3 |
| Isolation/purification methods |
Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient |
| Flotation density |
1.12-1.15 g/mL
|
| Molecules identified in the study |
Protein |
| Methods used in the study |
Mass spectrometry |
|
|
| Protein-protein interactions for ARHGAP18 |
|
|
| Pathways in which ARHGAP18 is involved |
|
|
|
