Experiment description of studies that identified EIF4ENIF1 in exosomes

1 Experiment ID 237 MISEV standards ✔ EM Biophysical techniques ✔ TSG101|Alix|HSC70|GAPDH Enriched markers ✔ HSP90B1 Negative markers ✔ qNano Particle analysis Identified molecule mRNA Identification method RNA Sequencing PubMed ID 26054723     Organism Homo sapiens Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N" Journal name Carcinogenesis Publication year 2015 Sample Hepatocytes Sample name MIHA Isolation/purification methods Differential centrifugation Filtration Ultracentrifugation Sucrose density gradient Flotation density 1.13-1.19 g/mL Molecules identified in the study Protein RNA Methods used in the study Western blotting Mass spectrometry RNA Sequencing

Protein-protein interactions for EIF4ENIF1

Protein Interactor ExoCarta ID Identification method PubMed Species 1 EIF4G3 8672 Affinity Capture-MS Homo sapiens 2 EIF3A 8661 Affinity Capture-MS Homo sapiens 3 C15orf39   Affinity Capture-MS Homo sapiens 4 UBE2H 7328 Affinity Capture-MS Homo sapiens 5 EXOSC7 23016 Two-hybrid Homo sapiens 6 PCGF5   Affinity Capture-MS Homo sapiens 7 HOOK1   Proximity Label-MS Homo sapiens 8 RPL15 6138 Two-hybrid Homo sapiens 9 DEF6 50619 Two-hybrid Homo sapiens 10 HELZ 9931 Affinity Capture-MS Homo sapiens 11 RBM22 55696 Two-hybrid Homo sapiens 12 EIF3C 8663 Affinity Capture-MS Homo sapiens 13 ARID3A   Affinity Capture-MS Homo sapiens 14 PKP2 5318 Two-hybrid Homo sapiens 15 DAPK1 1612 Two-hybrid Homo sapiens 16 Gtf2b   Affinity Capture-MS Mus musculus 17 DST 667 Two-hybrid Homo sapiens 18 QKI 9444 Two-hybrid Homo sapiens 19 MGAT5B   Two-hybrid Homo sapiens 20 Hsp90ab1 15516 Two-hybrid Mus musculus 21 KIF20A 10112 Affinity Capture-MS Homo sapiens 22 KPNA2 3838 Two-hybrid Homo sapiens 23 DHX57 90957 Two-hybrid Homo sapiens 24 EIF4EBP2 1979 Affinity Capture-MS Homo sapiens 25 Actb 11461 Two-hybrid Mus musculus 26 TAF1   Affinity Capture-MS Homo sapiens 27 EIF4E2   Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 28 SRSF8 10929 Two-hybrid Homo sapiens 29 Ssbp1   Two-hybrid Mus musculus 30 WTAP 9589 Two-hybrid Homo sapiens 31 FIS1 51024 Proximity Label-MS Homo sapiens 32 CDC16 8881 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 33 ELAVL3   Two-hybrid Homo sapiens 34 LLPH   Two-hybrid Homo sapiens 35 Tsc1   Affinity Capture-MS Mus musculus 36 Sumo1   Two-hybrid Mus musculus 37 EIF3B 8662 Affinity Capture-MS Homo sapiens 38 C8orf82   Proximity Label-MS Homo sapiens 39 CPSF7 79869 Two-hybrid Homo sapiens 40 SNRPC 6631 Affinity Capture-MS Homo sapiens 41 FBL 2091 Proximity Label-MS Homo sapiens 42 GUSBP5   Affinity Capture-MS Homo sapiens 43 ANGEL1   Affinity Capture-MS Homo sapiens 44 KRT31 3881 Two-hybrid Homo sapiens 45 EIF4G1 1981 Affinity Capture-MS Homo sapiens 46 SF3B3 23450 Two-hybrid Homo sapiens 47 EXD2   Proximity Label-MS Homo sapiens 48 TEX19   Affinity Capture-MS Homo sapiens 49 TRAK2 66008 Two-hybrid Homo sapiens 50 EEF1G 1937 Two-hybrid Homo sapiens 51 DDX6 1656 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens 52 OIP5   Two-hybrid Homo sapiens 53 CUL1 8454 Two-hybrid Homo sapiens 54 Eif1b   Two-hybrid Mus musculus 55 NPAS1   Affinity Capture-MS Homo sapiens 56 Eif5a 276770 Two-hybrid Mus musculus 57 PRR20C   Two-hybrid Homo sapiens 58 RPL19 6143 Two-hybrid Homo sapiens 59 PRR20A   Two-hybrid Homo sapiens 60 CPEB1   Proximity Label-MS Homo sapiens 61 KRT8 3856 Proximity Label-MS Homo sapiens 62 WBP4   Two-hybrid Homo sapiens 63 EIF3K 27335 Affinity Capture-MS Homo sapiens 64 Klc4   Two-hybrid Mus musculus 65 RC3H2   Affinity Capture-MS Homo sapiens 66 Rpl24 68193 Two-hybrid Mus musculus 67 LSM14B 149986 Affinity Capture-MS Homo sapiens 68 NOP9 161424 Two-hybrid Homo sapiens 69 CTBP1 1487 Affinity Capture-MS Homo sapiens 70 PNKP 11284 Two-hybrid Homo sapiens 71 SNRNP40 9410 Two-hybrid Homo sapiens 72 Nop16   Two-hybrid Mus musculus 73 TUBA1A 7846 Two-hybrid Homo sapiens 74 EIF3E 3646 Affinity Capture-MS Homo sapiens 75 PAN2   Affinity Capture-MS Homo sapiens 76 HIF1AN 55662 Affinity Capture-MS Homo sapiens 77 TTC19   Two-hybrid Homo sapiens 78 ANKHD1 54882 Two-hybrid Homo sapiens 79 SRBD1   Two-hybrid Homo sapiens 80 MYO10 4651 Two-hybrid Homo sapiens 81 RPL31 6160 Two-hybrid Homo sapiens 82 GLI3 2737 Proximity Label-MS Homo sapiens 83 TCHP   Two-hybrid Homo sapiens 84 DTX2 113878 Two-hybrid Homo sapiens Two-hybrid Homo sapiens 85 BICD2 23299 Proximity Label-MS Homo sapiens 86 RPA4   Proximity Label-MS Homo sapiens 87 BCL6   Affinity Capture-MS Homo sapiens 88 DDX1 1653 Two-hybrid Homo sapiens 89 SOWAHA   Affinity Capture-MS Homo sapiens 90 Nelfe   Two-hybrid Mus musculus 91 EIF4EBP1   Affinity Capture-MS Homo sapiens 92 LATS2 26524 Proximity Label-MS Homo sapiens 93 RHOT2 89941 Proximity Label-MS Homo sapiens 94 ATP5B 506 Two-hybrid Homo sapiens 95 C6orf165   Two-hybrid Homo sapiens 96 Rab6a 19346 Two-hybrid Mus musculus 97 EIF4A2 1974 Affinity Capture-MS Homo sapiens 98 EFHC1   Two-hybrid Homo sapiens Two-hybrid Homo sapiens 99 ATXN1 6310 Two-hybrid Homo sapiens 100 PHLPP1   Proximity Label-MS Homo sapiens 101 RBBP6 5930 Two-hybrid Homo sapiens 102 EIF3G 8666 Affinity Capture-MS Homo sapiens 103 GOLGA4   Two-hybrid Homo sapiens 104 CLK3   Two-hybrid Homo sapiens 105 XPO1 7514 Affinity Capture-MS Homo sapiens 106 BAG2 9532 Affinity Capture-MS Homo sapiens 107 SUPV3L1 6832 Two-hybrid Homo sapiens 108 RNF43   Proximity Label-MS Homo sapiens 109 Smad3   Affinity Capture-MS Mus musculus 110 CERK 64781 Affinity Capture-MS Homo sapiens 111 PHIP 55023 Affinity Capture-MS Homo sapiens 112 RPS7 6201 Two-hybrid Homo sapiens 113 PRR20D   Two-hybrid Homo sapiens 114 HOOK3 84376 Two-hybrid Homo sapiens 115 NUP43 348995 Proximity Label-MS Homo sapiens 116 EIF4E 1977 Two-hybrid Homo sapiens Two-hybrid Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens Two-hybrid Homo sapiens Far Western Homo sapiens Affinity Capture-Western Homo sapiens 117 DHX37   Two-hybrid Homo sapiens 118 DUSP16   Affinity Capture-MS Homo sapiens 119 POLR2A 5430 Two-hybrid Homo sapiens 120 KIAA0430   Affinity Capture-MS Homo sapiens 121 PRKCI 5584 Affinity Capture-MS Homo sapiens 122 CEP250 11190 Affinity Capture-MS Homo sapiens 123 PRR20B   Two-hybrid Homo sapiens 124 MYC   Affinity Capture-MS Homo sapiens 125 SNRPN 6638 Two-hybrid Homo sapiens 126 SAV1   Proximity Label-MS Homo sapiens 127 SEC16A 9919 Affinity Capture-MS Homo sapiens 128 EHMT2 10919 Two-hybrid Homo sapiens 129 HSPA1A 3303 Two-hybrid Homo sapiens 130 BICD1 636 Proximity Label-MS Homo sapiens 131 KIAA1671   Affinity Capture-MS Homo sapiens 132 Eif2s1 13665 Two-hybrid Mus musculus 133 NXF3   Two-hybrid Homo sapiens 134 CTNNA1 1495 Two-hybrid Homo sapiens 135 KRT19 3880 Two-hybrid Homo sapiens 136 LMNA 4000 Two-hybrid Homo sapiens 137 RPL26 6154 Two-hybrid Homo sapiens 138 FTSJ3 117246 Two-hybrid Homo sapiens 139 RAE1 8480 Two-hybrid Homo sapiens 140 BAG4   Two-hybrid Homo sapiens 141 EIF3D 8664 Affinity Capture-MS Homo sapiens 142 EIF3H 8667 Affinity Capture-MS Homo sapiens 143 TXLNA 200081 Two-hybrid Homo sapiens 144 ALG13 79868 Two-hybrid Homo sapiens 145 EIF3F 8665 Affinity Capture-MS Homo sapiens 146 ASB3 51130 Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 147 FBXW11   Affinity Capture-MS Homo sapiens 148 ANXA2 302 Two-hybrid Homo sapiens 149 BCAS2 10286 Two-hybrid Homo sapiens 150 HDGF 3068 Two-hybrid Homo sapiens 151 Srp54a   Two-hybrid Mus musculus 152 RPS21 6227 Two-hybrid Homo sapiens 153 EIF3I 8668 Affinity Capture-MS Homo sapiens 154 XRCC6 2547 Two-hybrid Homo sapiens 155 Utp18   Two-hybrid Mus musculus 156 KLHDC3   Affinity Capture-MS Homo sapiens Affinity Capture-MS Homo sapiens 157 AURKB 9212 Affinity Capture-MS Homo sapiens 158 FANCL 55120 Two-hybrid Homo sapiens 159 LSM14A 26065 Affinity Capture-MS Homo sapiens 160 BYSL 705 Two-hybrid Homo sapiens 161 ARHGAP18 93663 Affinity Capture-MS Homo sapiens 162 CREBBP   Affinity Capture-MS Homo sapiens 163 CAPZB 832 Affinity Capture-MS Homo sapiens 164 NISCH 11188 Affinity Capture-MS Homo sapiens 165 CDK6 1021 Biochemical Activity Homo sapiens 166 NUP35 129401 Proximity Label-MS Homo sapiens 167 PCGF3   Affinity Capture-MS Homo sapiens 168 RBFOX2 23543 Two-hybrid Homo sapiens 169 NUP50 10762 Affinity Capture-MS Homo sapiens 170 DDX11 1663 Two-hybrid Homo sapiens 171 CLASP1 23332 Two-hybrid Homo sapiens 172 Eif3g 53356 Two-hybrid Mus musculus 173 PRR20E   Two-hybrid Homo sapiens 174 HNRNPLL 92906 Two-hybrid Homo sapiens 175 ERF   Affinity Capture-MS Homo sapiens 176 DYNLRB1 83658 Proximity Label-MS Homo sapiens 177 RPS6 6194 Two-hybrid Homo sapiens 178 HNRNPF 3185 Two-hybrid Homo sapiens 179 RPRD1B 58490 Two-hybrid Homo sapiens 180 RPS27L 51065 Affinity Capture-MS Homo sapiens 181 SERBP1 26135 Proximity Label-MS Homo sapiens 182 EIF3M 10480 Affinity Capture-MS Homo sapiens 183 CCDC96   Affinity Capture-MS Homo sapiens 184 RC3H1 149041 Affinity Capture-MS Homo sapiens 185 POM121C   Affinity Capture-MS Homo sapiens 186 LUC7L 55692 Two-hybrid Homo sapiens 187 DDX28   Two-hybrid Homo sapiens 188 EIF3L 51386 Affinity Capture-MS Homo sapiens View the network

Pathways in which EIF4ENIF1 is involved

No pathways found