Gene description for Rab6a
Gene name RAB6A, member RAS oncogene family
Gene symbol Rab6a
Other names/aliases 2610028L11Rik
AA419671
Rab6
Species Mus musculus
 Database cross references - Rab6a
ExoCarta ExoCarta_19346
Vesiclepedia VP_19346
Entrez Gene 19346
UniProt P35279  
 Rab6a identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Embryonic fibroblasts 18494037    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rab6a
Molecular Function
    protein domain specific binding GO:0019904 ISO
    GTPase activity GO:0003924 ISO
    ATPase activator activity GO:0001671 ISO
    GTP binding GO:0005525 ISO
    nucleotide binding GO:0000166 IEA
    protein binding GO:0005515 IPI
    ATPase binding GO:0051117 ISO
    myosin V binding GO:0031489 ISO
Biological Process
    retrograde transport, endosome to Golgi GO:0042147 IBA
    vesicle-mediated transport GO:0016192 IEA
    protein targeting to Golgi GO:0000042 ISO
    protein localization to Golgi apparatus GO:0034067 ISO
    intracellular protein transport GO:0006886 TAS
    retrograde vesicle-mediated transport, Golgi to ER GO:0006890 IBA
    transport GO:0006810 IEA
    Rab protein signal transduction GO:0032482 IBA
    antigen processing and presentation GO:0019882 ISO
    small GTPase mediated signal transduction GO:0007264 IEA
    peptidyl-cysteine methylation GO:0018125 ISO
    positive regulation of ATPase activity GO:0032781 ISO
    intra-Golgi vesicle-mediated transport GO:0006891 IBA
    early endosome to Golgi transport GO:0034498 ISO
    protein transport GO:0015031 IEA
Subcellular Localization
    cytoplasmic vesicle GO:0031410 ISO
    Golgi apparatus GO:0005794 ISO
    membrane GO:0016020 ISO
    trans-Golgi network GO:0005802 ISO
    cytosol GO:0005829 ISO
    extracellular exosome GO:0070062 ISO
    cell GO:0005623 IBA
    intracellular GO:0005622 IBA
 Experiment description of studies that identified Rab6a in exosomes
1
Experiment ID 907
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 73
MISEV standards
EM
EV Biophysical techniques
HSP70|HSP90
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 18494037    
Organism Mus musculus
Experiment description Difference gel electrophoresis analysis of Ras-transformed fibroblast cell-derived exosomes.
Authors Ji H, Erfani N, Tauro BJ, Kapp EA, Zhu HJ, Moritz RL, Lim JW, Simpson RJ
Journal name ELEC
Publication year 2008
Sample Embryonic fibroblasts
Sample name NIH3T3
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LCQ DECA]
Western blotting
3
Experiment ID 188
MISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Enriched markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 908
MISEV standards
EM
EV Biophysical techniques
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
EV Enriched markers
HSPA5
EV Negative markers
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S.
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rab6a
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which Rab6a is involved
No pathways found





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