Gene description for HIF1AN
Gene name hypoxia inducible factor 1, alpha subunit inhibitor
Gene symbol HIF1AN
Other names/aliases FIH1
Species Homo sapiens
 Database cross references - HIF1AN
ExoCarta ExoCarta_55662
Vesiclepedia VP_55662
Entrez Gene 55662
HGNC 17113
MIM 606615
UniProt Q9NWT6  
 HIF1AN identified in exosomes derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
 Gene ontology annotations for HIF1AN
Molecular Function
    peptidyl-histidine dioxygenase activity GO:0036139 IDA
    cofactor binding GO:0048037 IDA
    NF-kappaB binding GO:0051059 IPI
    oxygen sensor activity GO:0019826 NAS
    protein binding GO:0005515 IPI
    protein homodimerization activity GO:0042803 IDA
    zinc ion binding GO:0008270 IDA
    Notch binding GO:0005112 IPI
    oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, 2-oxoglutarate as one donor, and incorporation of one atom each of oxygen into both donors GO:0016706 EXP
    ankyrin repeat binding GO:0071532 IPI
    carboxylic acid binding GO:0031406 IDA
    peptidyl-asparagine 3-dioxygenase activity GO:0036140 IMP
    iron ion binding GO:0005506 IDA
Biological Process
    negative regulation of Notch signaling pathway GO:0045746 IDA
    positive regulation of vasculogenesis GO:2001214 NAS
    positive regulation of myoblast differentiation GO:0045663 IDA
    oxidation-reduction process GO:0055114 IDA
    peptidyl-aspartic acid hydroxylation GO:0042264 IDA
    peptidyl-asparagine hydroxylation GO:0042265 IMP
    transcription, DNA-templated GO:0006351 IEA
    peptidyl-histidine hydroxylation GO:0036138 IDA
    negative regulation of transcription from RNA polymerase II promoter in response to hypoxia GO:0061428 IDA
    cellular response to hypoxia GO:0071456 TAS
    regulation of transcription from RNA polymerase II promoter in response to hypoxia GO:0061418 TAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    perinuclear region of cytoplasm GO:0048471 IDA
    nucleus GO:0005634 IDA
    nucleoplasm GO:0005654 IDA
 Experiment description of studies that identified HIF1AN in exosomes
1
Experiment ID 488
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
 Protein-protein interactions for HIF1AN
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 INVS  
Two-hybrid Homo sapiens
2 HDAC3 8841
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
3 ASB9  
Two-hybrid Homo sapiens
4 HDAC2 3066
Reconstituted Complex Homo sapiens
5 VHL  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
6 HDAC1 3065
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
7 FBF1  
Two-hybrid Homo sapiens
8 ASB13  
Two-hybrid Homo sapiens
9 HIF1A 3091
Affinity Capture-MS Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
View the network image/svg+xml
 Pathways in which HIF1AN is involved
PathwayEvidenceSource
Oxygen-dependent asparagine hydroxylation of Hypoxia-inducible Factor Alpha TAS Reactome





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