Gene description for UACA
Gene name uveal autoantigen with coiled-coil domains and ankyrin repeats
Gene symbol UACA
Other names/aliases NUCLING
Species Homo sapiens
 Database cross references - UACA
ExoCarta ExoCarta_55075
Vesiclepedia VP_55075
Entrez Gene 55075
HGNC 15947
MIM 612516
UniProt Q9BZF9  
 UACA identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Gastric cancer cells 33991177    
Hepatocellular carcinoma cells 26054723    
Mesenchymal stem cells 36408942    
Urine 19056867    
 Gene ontology annotations for UACA
Molecular Function
    actin binding GO:0003779 IEA
    protein binding GO:0005515 IPI
Biological Process
    intrinsic apoptotic signaling pathway in response to DNA damage GO:0008630 IBA
    intrinsic apoptotic signaling pathway in response to oxidative stress GO:0008631 IBA
    negative regulation of inflammatory response GO:0050728 IBA
    negative regulation of non-canonical NF-kappaB signal transduction GO:1901223 IBA
Subcellular Localization
    extracellular region GO:0005576 NAS
    nucleus GO:0005634 IBA
    nucleoplasm GO:0005654 IDA
    cytosol GO:0005829 IBA
    cytosol GO:0005829 IDA
    cytoskeleton GO:0005856 IEA
    extracellular exosome GO:0070062 HDA
 Experiment description of studies that identified UACA in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 363
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 364
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 365
MISEV standards
✘ Biophysical techniques
✔
TSG101|GAPDH|AQP1|CD151|CD81|CD82|CD9|EPCAM|FLOT1|FLOT2|ICAM1|ITGA2B|LAMP2|RAB35|RAB5A|RAB5B|SDCBP|TFRC|UCHL1
Enriched markers
✔
DCLK1
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 33991177    
Organism Homo sapiens
Experiment description Cancer stem cell marker DCLK1 reprograms small extracellular vesicles toward migratory phenotype in gastric cancer cells
Authors "Carli ALE, Afshar-Sterle S, Rai A, Fang H, O'Keefe R, Tse J, Ferguson FM, Gray NS, Ernst M, Greening DW, Buchert M."
Journal name Proteomics
Publication year 2021
Sample Gastric cancer cells
Sample name MKN1 - 100K pellet
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
8
Experiment ID 235
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
Enriched markers
✔
HSP90B1
Negative markers
✔
qNano
Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors "He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N"
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
9
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
10
Experiment ID 63
MISEV standards
✘ Biophysical techniques
✔
AQP2
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19056867    
Organism Homo sapiens
Experiment description Large-scale proteomics and phosphoproteomics of urinary exosomes.
Authors "Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA"
Journal name JASN
Publication year 2009
Sample Urine
Sample name Urine - Normal
Isolation/purification methods Differential centrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [LTQ]
Western blotting
 Protein-protein interactions for UACA
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ISG15 9636
Affinity Capture-MS Homo sapiens
2 PPP1CB 5500
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
3 OCLN 100506658
Proximity Label-MS Homo sapiens
4 RABEP2 79874
Affinity Capture-MS Homo sapiens
5 GJA1 2697
Proximity Label-MS Homo sapiens
6 PARD3 56288
Proximity Label-MS Homo sapiens
7 FBXO25  
Affinity Capture-MS Homo sapiens
8 FGF4  
Affinity Capture-MS Homo sapiens
9 TRIM33 51592
Affinity Capture-MS Homo sapiens
10 STX4 6810
Proximity Label-MS Homo sapiens
11 CORO6  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 CCDC102B  
Affinity Capture-MS Homo sapiens
13 APEX1 328
Affinity Capture-RNA Homo sapiens
14 BTN3A1 11119
Affinity Capture-MS Homo sapiens
15 PPP1R12A 4659
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
16 TRIOBP 11078
Affinity Capture-MS Homo sapiens
17 FBXW7  
Affinity Capture-MS Homo sapiens
18 KRT40  
Two-hybrid Homo sapiens
19 YWHAG 7532
Affinity Capture-MS Homo sapiens
20 YWHAH 7533
Affinity Capture-MS Homo sapiens
21 WIZ 58525
Affinity Capture-MS Homo sapiens
22 RAB35 11021
Proximity Label-MS Homo sapiens
23 SH3BGRL 6451
Affinity Capture-MS Homo sapiens
24 OCLM  
Affinity Capture-MS Homo sapiens
25 KIF14 9928
Affinity Capture-MS Homo sapiens
26 YWHAB 7529
Affinity Capture-MS Homo sapiens
27 C15orf59  
Affinity Capture-MS Homo sapiens
28 VTN 7448
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
29 USP48  
Co-fractionation Homo sapiens
30 MCC 4163
Affinity Capture-MS Homo sapiens
31 CTDP1 9150
Affinity Capture-MS Homo sapiens
32 RABEP1 9135
Co-fractionation Homo sapiens
33 LYN 4067
Proximity Label-MS Homo sapiens
34 MCAM 4162
Proximity Label-MS Homo sapiens
35 BAG6 7917
Affinity Capture-MS Homo sapiens
36 PNMA5 114824
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
37 DNAJC5 80331
Proximity Label-MS Homo sapiens
38 HIF1AN 55662
Two-hybrid Homo sapiens
39 RGS3 5998
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
40 CDH1 999
Proximity Label-MS Homo sapiens
41 YAP1 10413
Affinity Capture-MS Homo sapiens
42 MYH9 4627
Affinity Capture-MS Homo sapiens
43 TRIM21 6737
Affinity Capture-MS Homo sapiens
44 RILP 83547
Affinity Capture-MS Homo sapiens
45 SYNC  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
46 SYNCRIP 10492
Affinity Capture-MS Homo sapiens
47 YWHAQ 10971
Affinity Capture-MS Homo sapiens
48 ENTHD1  
Affinity Capture-MS Homo sapiens
49 STAMBP 10617
Two-hybrid Homo sapiens
50 RNASEH1 246243
Affinity Capture-MS Homo sapiens
51 ANLN 54443
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
52 PICK1  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
53 WHAMMP3  
Affinity Capture-MS Homo sapiens
54 AHCYL1 10768
Two-hybrid Homo sapiens
55 KIF20A 10112
Affinity Capture-MS Homo sapiens
56 JPH4 84502
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
57 RTP2  
Two-hybrid Homo sapiens
58 UBQLN2 29978
Two-hybrid Homo sapiens
59 CTNNB1 1499
Affinity Capture-MS Homo sapiens
60 MIS18A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
61 YWHAZ 7534
Affinity Capture-MS Homo sapiens
62 BTF3 689
Affinity Capture-MS Homo sapiens
63 GUCY1B3  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
64 FUS 2521
Affinity Capture-MS Homo sapiens
65 Otud5  
Affinity Capture-MS Mus musculus
66 NRD1 4898
Affinity Capture-MS Homo sapiens
67 TP63  
Affinity Capture-MS Homo sapiens
68 NDC80 10403
Proximity Label-MS Homo sapiens
69 RNF2  
Affinity Capture-MS Homo sapiens
70 RHOB 388
Proximity Label-MS Homo sapiens
71 L1TD1  
Affinity Capture-MS Homo sapiens
72 Ppp1cb 19046
Affinity Capture-MS Mus musculus
73 CCDC125 202243
Two-hybrid Homo sapiens
74 MED14  
Two-hybrid Homo sapiens
75 IFT22 64792
Affinity Capture-MS Homo sapiens
76 HIF1A 3091
Affinity Capture-MS Homo sapiens
77 WDR77 79084
Affinity Capture-MS Homo sapiens
78 CXCL6  
Affinity Capture-MS Homo sapiens
79 PRKAR1B  
Two-hybrid Homo sapiens
80 C11orf52 91894
Proximity Label-MS Homo sapiens
81 Mprip  
Affinity Capture-MS Mus musculus
82 BRK1 55845
Affinity Capture-MS Homo sapiens
83 WWP2 11060
Affinity Capture-MS Homo sapiens
84 RNF38  
Affinity Capture-MS Homo sapiens
85 TMEM257  
Affinity Capture-MS Homo sapiens
86 SORT1 6272
Affinity Capture-MS Homo sapiens
87 TIGD7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
88 GAGE5  
Affinity Capture-MS Homo sapiens
89 EPHA2 1969
Proximity Label-MS Homo sapiens
90 ZUFSP 221302
Affinity Capture-MS Homo sapiens
91 HNF1B  
Affinity Capture-MS Homo sapiens
92 KRAS 3845
Proximity Label-MS Homo sapiens
93 MYC  
Affinity Capture-MS Homo sapiens
94 C17orf59 54785
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Proximity Label-MS Homo sapiens
Affinity Capture-MS Homo sapiens
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