Gene description for IFT22
Gene name intraflagellar transport 22
Gene symbol IFT22
Other names/aliases RABL5
Species Homo sapiens
 Database cross references - IFT22
ExoCarta ExoCarta_64792
Vesiclepedia VP_64792
Entrez Gene 64792
HGNC 21895
UniProt Q9H7X7  
 IFT22 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for IFT22
Molecular Function
    GTPase activity GO:0003924 IBA
    GTP binding GO:0005525 IEA
Biological Process
    intracellular protein transport GO:0006886 IBA
    intraciliary anterograde transport GO:0035720 NAS
    cilium assembly GO:0060271 NAS
Subcellular Localization
    centrosome GO:0005813 IEA
    cilium GO:0005929 NAS
    cilium GO:0005929 TAS
    endomembrane system GO:0012505 IBA
    intraciliary transport particle B GO:0030992 IPI
    intraciliary transport particle B GO:0030992 ISS
    ciliary tip GO:0097542 TAS
 Experiment description of studies that identified IFT22 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IFT22
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IFT80 57560
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
2 HPSE2  
Affinity Capture-MS Homo sapiens
3 CCDC151  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 IFT57 55081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 TTC30A 92104
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
6 HOOK1  
Proximity Label-MS Homo sapiens
7 RAP1GDS1 5910
Affinity Capture-MS Homo sapiens
8 TTC26 79989
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
9 IFT20 90410
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 SMC4 10051
Affinity Capture-MS Homo sapiens
11 JPH4 84502
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 EIF4G1 1981
Affinity Capture-MS Homo sapiens
13 UBXN10  
Affinity Capture-MS Homo sapiens
14 IFT52 51098
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
15 DUSP3 1845
Affinity Capture-MS Homo sapiens
16 HSPB11 51668
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 TRAF3IP1 26146
Affinity Capture-MS Homo sapiens
18 ACAD11 84129
Affinity Capture-MS Homo sapiens
19 CLUAP1 23059
Affinity Capture-MS Homo sapiens
20 IFT27 11020
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
21 TRAT1 50852
Affinity Capture-MS Homo sapiens
22 UACA 55075
Affinity Capture-MS Homo sapiens
23 RBM33  
Co-fractionation Homo sapiens
24 TTC30B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
25 IFT46 56912
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
26 PARD6B 84612
Affinity Capture-MS Homo sapiens
27 IFT74 80173
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
28 BICD1 636
Proximity Label-MS Homo sapiens
29 TFPI2 7980
Affinity Capture-MS Homo sapiens
30 Tmem55a  
Affinity Capture-MS Mus musculus
31 IFT88 8100
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
32 RAP1A 5906
Affinity Capture-MS Homo sapiens
33 PRKY  
Affinity Capture-MS Homo sapiens
34 BICD2 23299
Proximity Label-MS Homo sapiens
35 SF3B6 51639
Affinity Capture-MS Homo sapiens
36 TEX37  
Affinity Capture-MS Homo sapiens
37 ARL13B 200894
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
38 SMC2 10592
Affinity Capture-MS Homo sapiens
39 ZC2HC1C  
Affinity Capture-MS Homo sapiens
40 RHOA 387
Affinity Capture-MS Homo sapiens
41 IFT81 28981
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 Pdlim5 56376
Affinity Capture-MS Mus musculus
43 DACT3  
Affinity Capture-MS Homo sapiens
44 IFT172 26160
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
45 CT45A5  
Affinity Capture-MS Homo sapiens
View the network image/svg+xml



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