Gene description for IFT88
Gene name intraflagellar transport 88
Gene symbol IFT88
Other names/aliases D13S1056E
DAF19
TG737
TTC10
hTg737
Species Homo sapiens
 Database cross references - IFT88
ExoCarta ExoCarta_8100
Vesiclepedia VP_8100
Entrez Gene 8100
HGNC 20606
MIM 600595
UniProt Q13099  
 IFT88 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for IFT88
Molecular Function
    kinesin binding GO:0019894 IEA
Biological Process
    regulation of smoothened signaling pathway GO:0008589 IEA
    response to fluid shear stress GO:0034405 IEA
    anterior/posterior pattern specification GO:0009952 IEA
    cardiac septum morphogenesis GO:0060411 IEA
    spermatid nucleus elongation GO:0007290 IEA
    cilium morphogenesis GO:0060271 ISS
    positive regulation of insulin-like growth factor receptor signaling pathway GO:0043568 IEA
    smoothened signaling pathway GO:0007224 IEA
    palate development GO:0060021 IEA
    pancreas development GO:0031016 IEA
    Notch signaling pathway GO:0007219 IEA
    inner ear receptor stereocilium organization GO:0060122 IEA
    negative regulation of epithelial cell proliferation GO:0050680 IEA
    cytoplasmic microtubule organization GO:0031122 IEA
    sperm axoneme assembly GO:0007288 IEA
    determination of left/right symmetry GO:0007368 IEA
    regulation of autophagosome assembly GO:2000785 ISS
    kidney development GO:0001822 IEA
    telencephalon development GO:0021537 IEA
    regulation of protein processing GO:0070613 IEA
    epithelial cell morphogenesis GO:0003382 IEA
    organelle organization GO:0006996 TAS
    heart formation GO:0060914 IEA
    eye development GO:0001654 IEA
    epidermal stem cell homeostasis GO:0036334 IEA
    regulation of cilium assembly GO:1902017 ISS
    liver development GO:0001889 IEA
    cochlea development GO:0090102 IEA
    cardiac muscle cell differentiation GO:0055007 IEA
    regulation of odontogenesis of dentin-containing tooth GO:0042487 IEA
    lung vasculature development GO:0060426 IEA
    regulation of fat cell differentiation GO:0045598 IEA
    protein localization GO:0008104 IEA
    forebrain morphogenesis GO:0048853 IEA
    embryonic digit morphogenesis GO:0042733 IEA
    epidermis development GO:0008544 IEA
    spinal cord dorsal/ventral patterning GO:0021513 IEA
Subcellular Localization
    ciliary tip GO:0097542 TAS
    intraciliary transport particle B GO:0030992 ISS
    ciliary basal body GO:0036064 ISS
    primary cilium GO:0072372 TAS
    motile cilium GO:0031514 ISS
    kinocilium GO:0060091 IEA
    acrosomal membrane GO:0002080 IEA
    apical part of cell GO:0045177 IEA
    ciliary base GO:0097546 IEA
    photoreceptor connecting cilium GO:0032391 IEA
    cilium GO:0005929 ISS
    axonemal basal plate GO:0097541 IEA
    motile primary cilium GO:0031512 IEA
    centriole GO:0005814 ISS
 Experiment description of studies that identified IFT88 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
EV Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
EV Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
EV Enriched markers
✔
CANX
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for IFT88
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PRRC2A 7916
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which IFT88 is involved
PathwayEvidenceSource
Hedgehog 'off' state TAS Reactome
Intraflagellar transport TAS Reactome





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