Gene description for CLUAP1
Gene name clusterin associated protein 1
Gene symbol CLUAP1
Other names/aliases CFAP22
FAP22
Species Homo sapiens
 Database cross references - CLUAP1
ExoCarta ExoCarta_23059
Vesiclepedia VP_23059
Entrez Gene 23059
HGNC 19009
UniProt Q96AJ1  
 CLUAP1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Retinal pigment epithelial cells 35333565    
Retinal pigment epithelial cells 35333565    
 Gene ontology annotations for CLUAP1
Molecular Function
    protein binding GO:0005515 IPI
Biological Process
    neural tube closure GO:0001843 IEA
    heart looping GO:0001947 IEA
    smoothened signaling pathway GO:0007224 IEA
    floor plate formation GO:0021508 IEA
    axoneme assembly GO:0035082 IEA
    intraciliary anterograde transport GO:0035720 NAS
    cilium assembly GO:0060271 IBA
    cilium assembly GO:0060271 NAS
Subcellular Localization
    nucleoplasm GO:0005654 IDA
    centrosome GO:0005813 IEA
    microtubule organizing center GO:0005815 IBA
    cilium GO:0005929 IBA
    cilium GO:0005929 NAS
    cilium GO:0005929 TAS
    intraciliary transport particle A GO:0030991 IEA
    intraciliary transport particle B GO:0030992 IBA
    intraciliary transport particle B GO:0030992 IPI
    intracellular membrane-bounded organelle GO:0043231 IDA
    ciliary tip GO:0097542 TAS
    ciliary base GO:0097546 IEA
 Experiment description of studies that identified CLUAP1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 834
MISEV standards
✔
EM
Biophysical techniques
✔
LAMP2|CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
6
Experiment ID 835
MISEV standards
✔
EM
Biophysical techniques
✔
CD63|FLOT1|ITGA2B|ICAM1|CD9|CD151|TFRC|RAB5A|GAPDH|AQP1|TSG101
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 35333565    
Organism Homo sapiens
Experiment description LAMP2A regulates the loading of proteins into exosomes
Authors "Ferreira JV, da Rosa Soares A, Ramalho J, Máximo Carvalho C, Cardoso MH, Pintado P, Carvalho AS, Beck HC, Matthiesen R, Zuzarte M, Girão H, van Niel G, Pereira P"
Journal name Sci Adv
Publication year 2022
Sample Retinal pigment epithelial cells
Sample name ARPE-19
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for CLUAP1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 IFT80 57560
Affinity Capture-MS Homo sapiens
2 GOLGA4  
Affinity Capture-MS Homo sapiens
3 IFT57 55081
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 TTC30A 92104
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
5 ICK  
Affinity Capture-MS Homo sapiens
6 IFT20 90410
Affinity Capture-MS Homo sapiens
7 CINP  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
8 MAGEA11  
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
9 UBXN10  
Reconstituted Complex Homo sapiens
Reconstituted Complex Homo sapiens
10 BBS7 55212
Affinity Capture-MS Homo sapiens
11 MYOG  
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
12 HSPB11 51668
Affinity Capture-MS Homo sapiens
13 VRK3 51231
Affinity Capture-MS Homo sapiens
14 IFT27 11020
Affinity Capture-MS Homo sapiens
15 UBE2E1  
Affinity Capture-MS Homo sapiens
16 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
17 CLU 1191
Affinity Capture-Western Homo sapiens
Affinity Capture-Western Homo sapiens
Two-hybrid Homo sapiens
18 SHMT2 6472
Affinity Capture-RNA Homo sapiens
19 TTC30B  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
20 NXF1 10482
Affinity Capture-RNA Homo sapiens
21 ABTB2 25841
Affinity Capture-MS Homo sapiens
22 IFT46 56912
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
23 BBS1 582
Affinity Capture-MS Homo sapiens
24 VCP 7415
Reconstituted Complex Homo sapiens
25 PCK1 5105
Affinity Capture-MS Homo sapiens
26 BBS9 27241
Affinity Capture-MS Homo sapiens
27 TFPI2 7980
Affinity Capture-MS Homo sapiens
28 IFT88 8100
Affinity Capture-MS Homo sapiens
29 KXD1 79036
Affinity Capture-MS Homo sapiens
30 TRAF3IP1 26146
Affinity Capture-MS Homo sapiens
31 IFT172 26160
Affinity Capture-MS Homo sapiens
32 CRACR2A  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 UBA6 55236
Affinity Capture-MS Homo sapiens
34 STX18 53407
Affinity Capture-MS Homo sapiens
35 BBS2 583
Affinity Capture-MS Homo sapiens
36 APP 351
Reconstituted Complex Homo sapiens
37 IFT22 64792
Affinity Capture-MS Homo sapiens
38 ASAP2 8853
Affinity Capture-MS Homo sapiens
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