Gene description for ASAP2
Gene name ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
Gene symbol ASAP2
Other names/aliases AMAP2
CENTB3
DDEF2
PAG3
PAP
Pap-alpha
SHAG1
Species Homo sapiens
 Database cross references - ASAP2
ExoCarta ExoCarta_8853
Vesiclepedia VP_8853
Entrez Gene 8853
HGNC 2721
MIM 603817
UniProt O43150  
 ASAP2 identified in sEVs derived from the following tissue/cell type
Mesenchymal stem cells 36408942    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for ASAP2
Molecular Function
    GTPase activator activity GO:0005096 IBA
    protein binding GO:0005515 IPI
    metal ion binding GO:0046872 IEA
Subcellular Localization
    cytosol GO:0005829 IDA
    plasma membrane GO:0005886 IDA
    Golgi cisterna membrane GO:0032580 IEA
 Experiment description of studies that identified ASAP2 in sEVs
1
Experiment ID 488
MISEV standards
EM
Biophysical techniques
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
CANX
Negative markers
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 231
MISEV standards
Biophysical techniques
Alix|CD63|CD9
Enriched markers
Negative markers
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
3
Experiment ID 232
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
4
Experiment ID 233
MISEV standards
Biophysical techniques
Enriched markers
Negative markers
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors "Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G."
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ASAP2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 KNDC1  
Affinity Capture-MS Homo sapiens
2 THSD7A 221981
Two-hybrid Homo sapiens
3 SEC24A 10802
Co-fractionation Homo sapiens
4 NCK1 4690
Affinity Capture-MS Homo sapiens
5 PARP1 142
Proximity Label-MS Homo sapiens
6 ITSN1 6453
Two-hybrid Homo sapiens
7 GRB2 2885
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 PDAP1 11333
Co-fractionation Homo sapiens
9 CLUAP1 23059
Affinity Capture-MS Homo sapiens
10 APC  
Reconstituted Complex Homo sapiens
11 ARHGAP12  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
12 Asap1  
Two-hybrid Mus musculus
13 ASAP3 55616
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
14 ADD3 120
Co-fractionation Homo sapiens
15 ELAVL1 1994
Affinity Capture-RNA Homo sapiens
16 NCK2 8440
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
17 DHX40  
Co-fractionation Homo sapiens
18 MAP1LC3A 84557
Affinity Capture-MS Homo sapiens
19 TBRG4 9238
Affinity Capture-MS Homo sapiens
20 Reps2  
Affinity Capture-Western Mus musculus
21 NTRK1 4914
Affinity Capture-MS Homo sapiens
22 PXN 5829
Reconstituted Complex Homo sapiens
23 PTK2B 2185
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
24 GAPDH 2597
Cross-Linking-MS (XL-MS) Homo sapiens
25 SRC 6714
Affinity Capture-Western Homo sapiens
26 SH3KBP1 30011
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Two-hybrid Homo sapiens
27 SRM 6723
Co-fractionation Homo sapiens
28 RAB11FIP3  
Affinity Capture-Western Homo sapiens
29 C19orf35  
Affinity Capture-MS Homo sapiens
30 KIAA1522 57648
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which ASAP2 is involved
No pathways found





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