Gene description for NCK2
Gene name NCK adaptor protein 2
Gene symbol NCK2
Other names/aliases GRB4
NCKbeta
Species Homo sapiens
 Database cross references - NCK2
ExoCarta ExoCarta_8440
Vesiclepedia VP_8440
Entrez Gene 8440
HGNC 7665
MIM 604930
UniProt O43639  
 NCK2 identified in exosomes derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Colorectal cancer cells 34887515    
Platelets 25332113    
Platelets 25332113    
Platelets 25332113    
 Gene ontology annotations for NCK2
Molecular Function
    receptor signaling complex scaffold activity GO:0030159 NAS
    protein binding GO:0005515 IPI
    SH3/SH2 adaptor activity GO:0005070 NAS
    cytoskeletal adaptor activity GO:0008093 NAS
Biological Process
    negative regulation of endoplasmic reticulum stress-induced eIF2 alpha phosphorylation GO:1903912 IDA
    epidermal growth factor receptor signaling pathway GO:0007173 TAS
    positive regulation of T cell proliferation GO:0042102 IMP
    positive regulation of signal transduction GO:0009967 NAS
    negative regulation of cell proliferation GO:0008285 TAS
    negative regulation of peptidyl-serine phosphorylation GO:0033137 IDA
    signal complex assembly GO:0007172 NAS
    axon guidance GO:0007411 TAS
    positive regulation of actin filament polymerization GO:0030838 IMP
    lamellipodium assembly GO:0030032 IEA
    vascular endothelial growth factor receptor signaling pathway GO:0048010 TAS
    cell migration GO:0016477 IEA
    ephrin receptor signaling pathway GO:0048013 TAS
    regulation of epidermal growth factor-activated receptor activity GO:0007176 TAS
    regulation of translation GO:0006417 IEA
    positive regulation of transcription from RNA polymerase II promoter GO:0045944 IDA
    signal transduction GO:0007165 TAS
    T cell activation GO:0042110 NAS
    actin filament organization GO:0007015 IEA
Subcellular Localization
    endoplasmic reticulum GO:0005783 IDA
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 TAS
    vesicle membrane GO:0012506 IEA
 Experiment description of studies that identified NCK2 in exosomes
1
Experiment ID 489
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
EV Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
EV Enriched markers
✔
Canx
EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ.
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 1203
MISEV standards
✔
EM
EV Biophysical techniques
✔
SDCBP|FLOT1|CD9|CD81|CD63|EPCAM|GAPDH|LAMP1|TFRC|CD151|CD82|LAMP2|RAB35|TSG101|FLOT2|RAB5B|ICAM1|RAB5A
EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 34887515    
Organism Homo sapiens
Experiment description Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets
Authors Zhang Q, Jeppesen DK, Higginbotham JN, Graves-Deal R, Trinh VQ, Ramirez MA, Sohn Y, Neininger AC, Taneja N, McKinley ET, Niitsu H, Cao Z, Evans R, Glass SE, Ray KC, Fissell WH, Hill S, Rose KL, Huh WJ, Washington MK, Ayers GD, Burnette DT, Sharma S, Rome LH, Franklin JL, Lee YA, Liu Q, Coffey RJ.
Journal name Nat Cell Biol
Publication year 2021
Sample Colorectal cancer cells
Sample name DiFi
Isolation/purification methods Differential centrifugation
Filtration
Centrifugal ultrafiltration
Ultracentrifugation
OptiPrep density gradient centrifugation
Flotation density -
Molecules identified in the study Protein
miRNA
Methods used in the study Western blotting
Mass spectrometry
RNA sequencing
6
Experiment ID 231
MISEV standards
✘ EV Biophysical techniques
✔
Alix|CD63|CD9
EV Enriched markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 1
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Lipids
Methods used in the study Western blotting
Mass spectrometry
7
Experiment ID 232
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 2
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
8
Experiment ID 233
MISEV standards
✘ EV Biophysical techniques
✘ EV Enriched markers
✘ EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 25332113    
Organism Homo sapiens
Experiment description Lipidomic and proteomic characterization of platelet extracellular vesicle subfractions from senescent platelets
Authors Pienimaeki-Roemer A, Kuhlmann K, Bottcher A, Konovalova T, Black A, Orso E, Liebisch G, Ahrens M, Eisenacher M, Meyer HE, Schmitz G.
Journal name Transfusion
Publication year 2015
Sample Platelets
Sample name PL-Exs - Rep 3
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Optiprep density gradient
Flotation density 1.12-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for NCK2
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 DOCK1 1793
Reconstituted Complex Homo sapiens
2 PKN2 5586
Invitro Homo sapiens
3 CBLB 868
Two-hybrid Homo sapiens
4 EEF1G 1937
Two-hybrid Homo sapiens
5 WIPF2 147179
Invivo Homo sapiens
6 CPSF6 11052
Two-hybrid Homo sapiens
7 PAK1 5058
Invivo Homo sapiens
Invitro Homo sapiens
8 WBP11  
Two-hybrid Homo sapiens
9 SF3B4 10262
Two-hybrid Homo sapiens
10 KIAA1217 56243
Two-hybrid Homo sapiens
11 LIMS1 3987
Affinity Capture-MS Homo sapiens
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
12 EFNB1 1947
Affinity Capture-MS Homo sapiens
13 SOS1 6654
Invivo Homo sapiens
Invitro Homo sapiens
14 NCKIPSD 51517
Two-hybrid Homo sapiens
15 PTK2 5747
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
16 PDGFRB 5159
Affinity Capture-MS Homo sapiens
Invitro Homo sapiens
Reconstituted Complex Homo sapiens
17 HNRNPK 3190
Two-hybrid Homo sapiens
Affinity Capture-MS Homo sapiens
18 DOK1 1796
Reconstituted Complex Homo sapiens
19 ABI2 10152
Two-hybrid Homo sapiens
20 TRIP6  
Two-hybrid Homo sapiens
21 FXR2 9513
Two-hybrid Homo sapiens
22 EGFR 1956
Invivo Homo sapiens
Reconstituted Complex Homo sapiens
23 CPSF7 79869
Two-hybrid Homo sapiens
24 ILK 3611
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
25 CD3E 916
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
26 NTRK2 4915
Invitro Homo sapiens
Two-hybrid Homo sapiens
View the network image/svg+xml



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