Gene description for MICAL1
Gene name microtubule associated monooxygenase, calponin and LIM domain containing 1
Gene symbol MICAL1
Other names/aliases MICAL
MICAL-1
NICAL
Species Homo sapiens
 Database cross references - MICAL1
ExoCarta ExoCarta_64780
Vesiclepedia VP_64780
Entrez Gene 64780
HGNC 20619
MIM 607129
UniProt Q8TDZ2  
 MICAL1 identified in sEVs derived from the following tissue/cell type
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Bone marrow mesenchymal stem cells 36408942    
Mesenchymal stem cells 36408942    
Thymus 23844026    
 Gene ontology annotations for MICAL1
Molecular Function
    actin binding GO:0003779 IDA
    monooxygenase activity GO:0004497 IDA
    protein binding GO:0005515 IPI
    NAD(P)H oxidase H2O2-forming activity GO:0016174 IDA
    oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen GO:0016709 IDA
    SH3 domain binding GO:0017124 IPI
    protein kinase binding GO:0019901 IEA
    small GTPase binding GO:0031267 IEA
    metal ion binding GO:0046872 IEA
    actin filament binding GO:0051015 IBA
    FAD binding GO:0071949 IDA
    NADPH oxidase H202-forming activity GO:0106294 IEA
    F-actin monooxygenase activity GO:0120501 IEA
Biological Process
    cytoskeleton organization GO:0007010 NAS
    signal transduction GO:0007165 NAS
    sulfur oxidation GO:0019417 ISS
    actin filament depolymerization GO:0030042 IDA
    negative regulation of apoptotic process GO:0043066 IEA
    actin filament bundle assembly GO:0051017 IBA
    regulation of regulated secretory pathway GO:1903305 IEA
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 IDA
    cytosol GO:0005829 TAS
    intermediate filament GO:0005882 NAS
    actin filament GO:0005884 IDA
    plasma membrane GO:0005886 IBA
    endosome membrane GO:0010008 IDA
    actin cytoskeleton GO:0015629 IBA
    midbody GO:0030496 IDA
    intercellular bridge GO:0045171 IDA
    hippocampal mossy fiber expansion GO:1990026 IEA
 Experiment description of studies that identified MICAL1 in sEVs
1
Experiment ID 489
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 6
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
2
Experiment ID 490
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 7
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
3
Experiment ID 491
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
4
Experiment ID 492
MISEV standards
✔
EM
Biophysical techniques
✔
Cd9|Cd81|Cd63|Gapdh|Sdcbp|Lamp1|Aqp1|Rab5a|Icam1|Cd82|Itga2b|Tsg101|Lamp2|Rab35|Flot1|Flot2|Cd151|Rab5b|Tfrc|Uchl1
Enriched markers
✔
Canx
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Rattus norvegicus
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Bone marrow mesenchymal stem cells
Sample name BMSC - Passage 9
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
5
Experiment ID 488
MISEV standards
✔
EM
Biophysical techniques
✔
CD9|CD81|CD63|GAPDH|SDCBP|LAMP1|TFRC|UCHL1|FLOT2|LAMP2|FLOT1|ICAM1|RAB5B|CD151|RAB35|TSG101|RAB5A|CD82
Enriched markers
✔
CANX
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 36408942    
Organism Homo sapiens
Experiment description Quantitative proteomic analysis of exosomes from umbilical cord mesenchymal stem cells and rat bone marrow stem cells
Authors "Xu X, Yin F, Guo M, Gan G, Lin G, Wen C, Wang J, Song P, Wang J, Qi ZQ, Zhong CQ."
Journal name Proteomics
Publication year 2023
Sample Mesenchymal stem cells
Sample name UCMSC
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectometry
6
Experiment ID 217
MISEV standards
✔
EM
Biophysical techniques
✔
TSG101|CD81|CD9|CD63
Enriched markers
✘ Negative markers
✔
NTA
Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors "Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O."
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for MICAL1
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 ATG16L1 55054
Affinity Capture-MS Homo sapiens
2 RAB37 326624
Affinity Capture-MS Homo sapiens
3 SERHL2  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
4 MIER3 166968
Affinity Capture-MS Homo sapiens
5 KIF23 9493
Affinity Capture-MS Homo sapiens
6 NPAS1  
Affinity Capture-MS Homo sapiens
7 USH1G  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
8 MOSPD3  
Affinity Capture-MS Homo sapiens
9 CASS4  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
10 NCSTN 23385
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
11 GRB2 2885
Reconstituted Complex Homo sapiens
12 EHD1 10938
Reconstituted Complex Homo sapiens
Two-hybrid Homo sapiens
Two-hybrid Homo sapiens
13 GSK3B 2932
Two-hybrid Homo sapiens
14 CCDC115 84317
Affinity Capture-MS Homo sapiens
15 TAS2R7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
16 VIM 7431
Affinity Capture-Western Homo sapiens
17 GADD45A  
Two-hybrid Homo sapiens
18 CRK 1398
Reconstituted Complex Homo sapiens
19 ABL1 25
Reconstituted Complex Homo sapiens
20 SPG21 51324
Affinity Capture-MS Homo sapiens
21 DACT1 51339
Affinity Capture-MS Homo sapiens
22 TAF3 83860
Affinity Capture-MS Homo sapiens
23 TMEM88  
Affinity Capture-MS Homo sapiens
24 TAB1 10454
Two-hybrid Homo sapiens
25 GIMAP6  
Affinity Capture-MS Homo sapiens
26 PSORS1C2  
Affinity Capture-MS Homo sapiens
27 GPAM 57678
Affinity Capture-MS Homo sapiens
28 FAM120AOS  
Affinity Capture-MS Homo sapiens
29 FBXL17  
Affinity Capture-MS Homo sapiens
30 GUCY2F  
Affinity Capture-MS Homo sapiens
31 PCGF3  
Affinity Capture-MS Homo sapiens
32 SLC25A41  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
33 KIAA0895  
Affinity Capture-MS Homo sapiens
34 STC2 8614
Affinity Capture-MS Homo sapiens
35 NCK2 8440
Affinity Capture-MS Homo sapiens
36 TLR9  
Affinity Capture-MS Homo sapiens
37 PSPN  
Affinity Capture-MS Homo sapiens
38 CACUL1  
Affinity Capture-MS Homo sapiens
39 CERS2 29956
Affinity Capture-MS Homo sapiens
40 GJB7  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
41 ARHGAP26 23092
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
42 RINL  
Affinity Capture-MS Homo sapiens
43 GP1BB 2812
Affinity Capture-MS Homo sapiens
44 ARMS2 387715
Affinity Capture-MS Homo sapiens
45 NTRK1 4914
Affinity Capture-MS Homo sapiens
46 NEDD9 4739
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
Affinity Capture-Western Homo sapiens
47 GNAO1 2775
Affinity Capture-MS Homo sapiens
48 ZNF517  
Affinity Capture-MS Homo sapiens
Affinity Capture-MS Homo sapiens
49 CENPM  
Affinity Capture-MS Homo sapiens
50 HBG2 3048
Affinity Capture-MS Homo sapiens
51 CSNK2B 1460
Affinity Capture-MS Homo sapiens
52 SORBS3 10174
Affinity Capture-MS Homo sapiens
53 ZNF641  
Affinity Capture-MS Homo sapiens
54 IL1RL2 8808
Affinity Capture-MS Homo sapiens
55 SRC 6714
Reconstituted Complex Homo sapiens
56 TBCA 6902
Affinity Capture-MS Homo sapiens
57 RAB10 10890
Two-hybrid Homo sapiens
58 SIRT3  
Affinity Capture-MS Homo sapiens
59 RPS6KA6 27330
Two-hybrid Homo sapiens
60 RAB1A 5861
Two-hybrid Homo sapiens
Reconstituted Complex Homo sapiens
61 HIST1H2AB 8335
Affinity Capture-MS Homo sapiens
62 CTTN 2017
Cross-Linking-MS (XL-MS) Homo sapiens
63 PIK3R3 8503
Two-hybrid Homo sapiens
View the network image/svg+xml
 Pathways in which MICAL1 is involved
PathwayEvidenceSource
Factors involved in megakaryocyte development and platelet production TAS Reactome
Hemostasis TAS Reactome





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