Gene description for ARHGAP26
Gene name Rho GTPase activating protein 26
Gene symbol ARHGAP26
Other names/aliases GRAF
GRAF1
OPHN1L
OPHN1L1
Species Homo sapiens
 Database cross references - ARHGAP26
ExoCarta ExoCarta_23092
Entrez Gene 23092
HGNC 17073
MIM 605370
UniProt Q9UNA1  
 ARHGAP26 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Thymus 23844026    
 Gene ontology annotations for ARHGAP26
Molecular Function
    GTPase activator activity GO:0005096 NAS
    protein binding GO:0005515 IPI
    phospholipid binding GO:0005543 IDA
Biological Process
    regulation of small GTPase mediated signal transduction GO:0051056 TAS
    positive regulation of GTPase activity GO:0043547 NAS
    plasma membrane organization GO:0007009 IEA
    actin cytoskeleton organization GO:0030036 NAS
    nervous system development GO:0007399 NAS
    small GTPase mediated signal transduction GO:0007264 TAS
Subcellular Localization
    cellular_component GO:0005575 ND
    focal adhesion GO:0005925 IEA
    cytosol GO:0005829 TAS
    cytoskeleton GO:0005856 IEA
 Experiment description of studies that identified ARHGAP26 in exosomes
1
Experiment ID 235
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
✘ EV Membrane markers
✔
HSP90B1
EV Negative markers
✔
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 217
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101
EV Cytosolic markers
✔
CD81|CD9|CD63
EV Membrane markers
✘ EV Negative markers
✔
NTA
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23844026    
Organism Homo sapiens
Experiment description Characterization of human thymic exosomes.
Authors Skogberg G, Gudmundsdottir J, van der Post S, Sandstrom K, Bruhn S, Benson M, Mincheva-Nilsson L, Baranov V, Telemo E, Ekwall O.
Journal name PLoS One
Publication year 2013
Sample Thymus
Sample name Normal-Thymus
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for ARHGAP26
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PTK2 5747
Reconstituted Complex Homo sapiens
2 RHOA 387
Biochemical Activity Homo sapiens
3 PKN3 29941
Two-hybrid Homo sapiens
Affinity Capture-Western Homo sapiens
Reconstituted Complex Homo sapiens
4 CDC42 998
Biochemical Activity Homo sapiens
View the network image/svg+xml
 Pathways in which ARHGAP26 is involved
PathwayEvidenceSource
Rho GTPase cycle TAS Reactome





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