Gene description for CSNK1D
Gene name casein kinase 1, delta
Gene symbol CSNK1D
Other names/aliases ASPS
CKIdelta
FASPS2
HCKID
Species Homo sapiens
 Database cross references - CSNK1D
ExoCarta ExoCarta_1453
Entrez Gene 1453
HGNC 2452
MIM 600864
UniProt P48730  
 CSNK1D identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for CSNK1D
Molecular Function
    protein serine/threonine kinase activity GO:0004674 IBA
    ATP binding GO:0005524 IEA
    tau-protein kinase activity GO:0050321 IEA
    protein binding GO:0005515 IPI
    peptide binding GO:0042277 IEA
    phosphoprotein binding GO:0051219 IEA
    glycoprotein binding GO:0001948 IEA
    protein kinase activity GO:0004672 TAS
Biological Process
    endocytosis GO:0006897 IBA
    positive regulation of canonical Wnt signaling pathway GO:0090263 IMP
    DNA repair GO:0006281 TAS
    protein phosphorylation GO:0006468 IDA
    circadian regulation of gene expression GO:0032922 ISS
    positive regulation of protein phosphorylation GO:0001934 IMP
    mitotic cell cycle GO:0000278 TAS
    positive regulation of proteasomal ubiquitin-dependent protein catabolic process GO:0032436 ISS
    Wnt signaling pathway GO:0016055 IBA
    signal transduction GO:0007165 TAS
    peptidyl-serine phosphorylation GO:0018105 IBA
    regulation of circadian rhythm GO:0042752 ISS
    organelle organization GO:0006996 TAS
    G2/M transition of mitotic cell cycle GO:0000086 TAS
    spindle assembly GO:0051225 IDA
    regulation of cell shape GO:0008360 IBA
Subcellular Localization
    cytoplasm GO:0005737 IBA
    cytosol GO:0005829 TAS
    centrosome GO:0005813 IDA
    Golgi apparatus GO:0005794 IDA
    plasma membrane GO:0005886 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    spindle microtubule GO:0005876 IDA
    nucleus GO:0005634 ISS
    spindle GO:0005819 IDA
 Experiment description of studies that identified CSNK1D in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
2
Experiment ID 211
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
cytochrome c|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
3
Experiment ID 212
ISEV standards
CEM
EV Biophysical techniques
TSG101|Alix
EV Cytosolic markers
EpCAM|TFRC
EV Membrane markers
Cytochrome C|GOLGA2
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for CSNK1D
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 PER1  
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
2 PER3  
Invivo Homo sapiens
Affinity Capture-MS Homo sapiens
3 AKAP9 10142
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
4 DVL1 1855
Invivo Homo sapiens
Invitro Homo sapiens
Two-hybrid Homo sapiens
5 MCC 4163
Affinity Capture-MS Homo sapiens
6 ARFGAP1  
Invitro Homo sapiens
Affinity Capture-MS Homo sapiens
7 DVL3  
Two-hybrid Homo sapiens
8 TP53  
Affinity Capture-MS Homo sapiens
9 GJA1 2697
Affinity Capture-Western Homo sapiens
Affinity Capture-MS Homo sapiens
Biochemical Activity Homo sapiens
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