Gene description for GTPBP4
Gene name GTP binding protein 4
Gene symbol GTPBP4
Other names/aliases CRFG
NGB
NOG1
Species Homo sapiens
 Database cross references - GTPBP4
ExoCarta ExoCarta_23560
Entrez Gene 23560
HGNC 21535
UniProt Q9BZE4  
 GTPBP4 identified in exosomes derived from the following tissue/cell type
Hepatocellular carcinoma cells 26054723    
 Gene ontology annotations for GTPBP4
Molecular Function
    GTPase activity GO:0003924 IDA
    poly(A) RNA binding GO:0044822 IDA
    GTP binding GO:0005525 IDA
    protein binding GO:0005515 IPI
Biological Process
    negative regulation of cell-cell adhesion GO:0022408 IDA
    negative regulation of cell proliferation GO:0008285 IMP
    protein stabilization GO:0050821 IDA
    ribosome biogenesis GO:0042254 IEA
    negative regulation of cell migration GO:0030336 IDA
    osteoblast differentiation GO:0001649 IDA
    negative regulation of DNA replication GO:0008156 IMP
    negative regulation of collagen binding GO:0033342 IMP
    metabolic process GO:0008152 IDA
    regulation of cyclin-dependent protein serine/threonine kinase activity GO:0000079 IDA
    negative regulation of protein ubiquitination GO:0031397 IDA
Subcellular Localization
    membrane GO:0016020 IDA
    nucleus GO:0005634 IDA
    perinuclear region of cytoplasm GO:0048471 IDA
    nucleolus GO:0005730 IDA
    Golgi apparatus GO:0005794 IDA
    cytoplasm GO:0005737 IDA
    nuclear membrane GO:0031965 IDA
 Experiment description of studies that identified GTPBP4 in exosomes
1
Experiment ID 235
ISEV standards
EM
EV Biophysical techniques
TSG101|Alix|HSC70|GAPDH
EV Cytosolic markers
EV Membrane markers
HSP90B1
EV Negative markers
qNano
EV Particle analysis
Identified molecule mRNA
Identification method RNA Sequencing
PubMed ID 26054723    
Organism Homo sapiens
Experiment description Hepatocellular carcinoma-derived exosomes promote motility of immortalized hepatocyte through transfer of oncogenic proteins and RNAs
Authors He M, Qin H, Poon TC, Sze SC, Ding X, Co NN, Ngai SM, Chan TF, Wong N
Journal name Carcinogenesis
Publication year 2015
Sample Hepatocellular carcinoma cells
Sample name HKCI-8
Isolation/purification methods Differential centrifugation
Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.13-1.19 g/mL
Molecules identified in the study Protein
RNA
Methods used in the study Western blotting
Mass spectrometry
RT-PCR
RNA Sequencing
 Protein-protein interactions for GTPBP4
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 EIF6 3692
Affinity Capture-MS Homo sapiens
2 PINX1  
Affinity Capture-MS Homo sapiens
3 LYAR 55646
Affinity Capture-MS Homo sapiens
View the network image/svg+xml
 Pathways in which GTPBP4 is involved
No pathways found





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