Gene description for DDX47
Gene name DEAD (Asp-Glu-Ala-Asp) box polypeptide 47
Gene symbol DDX47
Other names/aliases E4-DBP
HQ0256
MSTP162
RRP3
Species Homo sapiens
 Database cross references - DDX47
ExoCarta ExoCarta_51202
Entrez Gene 51202
HGNC 18682
MIM 615428
UniProt Q9H0S4  
 DDX47 identified in exosomes derived from the following tissue/cell type
Ovarian cancer cells 23333927    
Ovarian cancer cells 23333927    
 Gene ontology annotations for DDX47
Molecular Function
    ATP-dependent RNA helicase activity GO:0004004 IBA
    protein binding GO:0005515 IPI
    ATP binding GO:0005524 IEA
    poly(A) RNA binding GO:0044822 IDA
Biological Process
    mRNA processing GO:0006397 IEA
    rRNA processing GO:0006364 IMP
    extrinsic apoptotic signaling pathway via death domain receptors GO:0008625 IDA
    RNA splicing GO:0008380 IMP
    RNA secondary structure unwinding GO:0010501 IBA
Subcellular Localization
    membrane GO:0016020 IDA
    nucleolus GO:0005730 IDA
 Experiment description of studies that identified DDX47 in exosomes
1
Experiment ID 211
ISEV standards
✔
EM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
cytochrome c|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name IGROV1
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
2
Experiment ID 212
ISEV standards
✔
CEM
EV Biophysical techniques
✔
TSG101|Alix
EV Cytosolic markers
✔
EpCAM|TFRC
EV Membrane markers
✔
Cytochrome C|GOLGA2
EV Negative markers
✘ EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 23333927    
Organism Homo sapiens
Experiment description Characterization and proteomic analysis of ovarian cancer-derived exosomes.
Authors Liang B, Peng P, Chen S, Li L, Zhang M, Cao D, Yang J, Li H, Gui T, Li X, Shen K.
Journal name J Proteomics
Publication year 2013
Sample Ovarian cancer cells
Sample name OVCAR-3
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Sucrose density gradient
Flotation density 1.09-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for DDX47
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.
 Pathways in which DDX47 is involved
No pathways found





Perform bioinformatics analysis of your extracellular vesicle data set using FunRich, a open access standalone tool. NEW UPDATED VERSION OF FunRich available for download (12/09/2016) from here