Gene description for Rpl35
Gene name ribosomal protein L35
Gene symbol Rpl35
Other names/aliases 2410039E09Rik
Species Mus musculus
 Database cross references - Rpl35
ExoCarta ExoCarta_66489
Vesiclepedia VP_66489
Entrez Gene 66489
UniProt Q6ZWV7  
 Rpl35 identified in exosomes derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl35
Molecular Function
    mRNA binding GO:0003729 IBA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
    protein binding GO:0005515 IPI
    ribonucleoprotein complex binding GO:0043021 ISO
Biological Process
    maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000463 IBA
    maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000463 IEA
    cytoplasmic translation GO:0002181 NAS
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
Subcellular Localization
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
 Experiment description of studies that identified Rpl35 in exosomes
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl35
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps3 27050
Co-fractionation Mus musculus
2 Rpl28 19943
Co-fractionation Mus musculus
3 RPS12 6206
Affinity Capture-MS Homo sapiens
4 MPG 4350
Affinity Capture-MS Homo sapiens
5 RPSA 3921
Affinity Capture-MS Homo sapiens
6 WDR6 11180
Affinity Capture-MS Homo sapiens
7 EIF3A 8661
Affinity Capture-MS Homo sapiens
8 PEG10 23089
Affinity Capture-MS Homo sapiens
9 FAM98A 25940
Affinity Capture-MS Homo sapiens
10 GTF3C5 9328
Affinity Capture-MS Homo sapiens
11 RPS3A 6189
Affinity Capture-MS Homo sapiens
12 SKIV2L 6499
Affinity Capture-MS Homo sapiens
13 KAT6A  
Affinity Capture-MS Homo sapiens
14 EIF3C 8663
Affinity Capture-MS Homo sapiens
15 RPL30 6156
Affinity Capture-MS Homo sapiens
16 RPS17 6218
Affinity Capture-MS Homo sapiens
17 NKRF 55922
Affinity Capture-MS Homo sapiens
18 USP10 9100
Affinity Capture-MS Homo sapiens
19 RCC2 55920
Affinity Capture-MS Homo sapiens
20 BTF3 689
Affinity Capture-MS Homo sapiens
21 RPL13A 23521
Affinity Capture-MS Homo sapiens
22 Rpl27 19942
Co-fractionation Mus musculus
23 G3BP1 10146
Affinity Capture-MS Homo sapiens
24 LARP1B 55132
Affinity Capture-MS Homo sapiens
25 Rpl6 19988
Co-fractionation Mus musculus
26 RPS19 6223
Affinity Capture-MS Homo sapiens
27 RPL10 6134
Affinity Capture-MS Homo sapiens
28 TDRD3  
Affinity Capture-MS Homo sapiens
29 GTPBP1 9567
Affinity Capture-MS Homo sapiens
30 MRPL50 54534
Affinity Capture-MS Homo sapiens
31 Rps24 20088
Co-fractionation Mus musculus
32 MED24 9862
Affinity Capture-MS Homo sapiens
33 CCDC124 115098
Affinity Capture-MS Homo sapiens
34 DECR1 1666
Affinity Capture-MS Homo sapiens
35 FAU 2197
Affinity Capture-MS Homo sapiens
36 RPS3 6188
Affinity Capture-MS Homo sapiens
37 DNAJB6 10049
Affinity Capture-MS Homo sapiens
38 Tmem173  
Proximity Label-MS Mus musculus
39 RPS18 6222
Affinity Capture-MS Homo sapiens
40 MGA  
Affinity Capture-MS Homo sapiens
41 SRP54 6729
Affinity Capture-MS Homo sapiens
42 TBL2 26608
Affinity Capture-MS Homo sapiens
43 VRK2 7444
Affinity Capture-MS Homo sapiens
44 Rpl7 19989
Co-fractionation Mus musculus
45 MRPL48  
Affinity Capture-MS Homo sapiens
46 Rps9 76846
Co-fractionation Mus musculus
47 MRPS34 65993
Affinity Capture-MS Homo sapiens
48 RPL7A 6130
Affinity Capture-MS Homo sapiens
49 RPL24 6152
Affinity Capture-MS Homo sapiens
50 LPGAT1 9926
Affinity Capture-MS Homo sapiens
51 RBM3 5935
Affinity Capture-MS Homo sapiens
52 CFAP97  
Affinity Capture-MS Homo sapiens
53 RRP12 23223
Affinity Capture-MS Homo sapiens
54 Rpl36a 19982
Co-fractionation Mus musculus
55 Rpl26 19941
Co-fractionation Mus musculus
56 RFC3 5983
Affinity Capture-MS Homo sapiens
57 BRCC3 79184
Affinity Capture-MS Homo sapiens
58 TIMM23 100287932
Affinity Capture-MS Homo sapiens
59 RIOK2 55781
Affinity Capture-MS Homo sapiens
60 NPM1 4869
Affinity Capture-MS Homo sapiens
61 LSG1  
Affinity Capture-MS Homo sapiens
62 EIF5B 9669
Affinity Capture-MS Homo sapiens
63 RPS5 6193
Affinity Capture-MS Homo sapiens
64 EIF3F 8665
Affinity Capture-MS Homo sapiens
65 Rpl3 27367
Co-fractionation Mus musculus
66 SPIN2A  
Affinity Capture-MS Homo sapiens
67 NSUN4  
Affinity Capture-MS Homo sapiens
68 ADARB1 104
Affinity Capture-MS Homo sapiens
69 EIF3E 3646
Affinity Capture-MS Homo sapiens
70 EED  
Affinity Capture-MS Homo sapiens
71 PTPN14 5784
Affinity Capture-MS Homo sapiens
72 DDX18 8886
Affinity Capture-MS Homo sapiens
73 Rps7 20115
Co-fractionation Mus musculus
74 Rpl11 67025
Co-fractionation Mus musculus
75 MTDH 92140
Affinity Capture-MS Homo sapiens
76 ZNF346  
Affinity Capture-MS Homo sapiens
77 Rpl31 114641
Co-fractionation Mus musculus
78 Rpl17 319195
Co-fractionation Mus musculus
79 ZNF622 90441
Affinity Capture-MS Homo sapiens
80 Rps10 67097
Co-fractionation Mus musculus
81 RPL19 6143
Affinity Capture-MS Homo sapiens
82 Rplp2 67186
Co-fractionation Mus musculus
83 TRMT10C 54931
Affinity Capture-MS Homo sapiens
84 Rps4x 20102
Co-fractionation Mus musculus
85 LARP4B 23185
Affinity Capture-MS Homo sapiens
86 EEFSEC 60678
Affinity Capture-MS Homo sapiens
87 IMPDH1 3614
Affinity Capture-MS Homo sapiens
88 MEPCE 56257
Affinity Capture-MS Homo sapiens
89 TFB1M  
Affinity Capture-MS Homo sapiens
90 HEXIM1 10614
Affinity Capture-MS Homo sapiens
91 Rps23 66475
Co-fractionation Mus musculus
92 TTC37 9652
Affinity Capture-MS Homo sapiens
93 RPL27 6155
Affinity Capture-MS Homo sapiens
94 RPS9 6203
Affinity Capture-MS Homo sapiens
95 EIF3K 27335
Affinity Capture-MS Homo sapiens
96 TRIM7  
Affinity Capture-MS Homo sapiens
97 TSR1 55720
Affinity Capture-MS Homo sapiens
98 Eif3d 55944
Co-fractionation Mus musculus
99 MED9  
Affinity Capture-MS Homo sapiens
100 DDX21 9188
Affinity Capture-MS Homo sapiens
101 MRPL45 84311
Affinity Capture-MS Homo sapiens
102 RFC5 5985
Affinity Capture-MS Homo sapiens
103 RPL4 6124
Affinity Capture-MS Homo sapiens
104 RBM6 10180
Affinity Capture-MS Homo sapiens
105 DRG1 4733
Affinity Capture-MS Homo sapiens
106 DERA 51071
Affinity Capture-MS Homo sapiens
107 BTF3L4 91408
Affinity Capture-MS Homo sapiens
108 BLM 641
Affinity Capture-MS Homo sapiens
109 NONO 4841
Affinity Capture-MS Homo sapiens
110 CPS1 1373
Affinity Capture-MS Homo sapiens
111 BAIAP2 10458
Affinity Capture-MS Homo sapiens
112 ZMYM3  
Affinity Capture-MS Homo sapiens
113 RNF213 57674
Affinity Capture-MS Homo sapiens
114 OSBPL3 26031
Affinity Capture-MS Homo sapiens
115 Rpl24 68193
Co-fractionation Mus musculus
116 RPL5 6125
Affinity Capture-MS Homo sapiens
117 RPL18A 6142
Affinity Capture-MS Homo sapiens
118 Rps5 20103
Co-fractionation Mus musculus
119 MNAT1  
Affinity Capture-MS Homo sapiens
120 CEP170 9859
Affinity Capture-MS Homo sapiens
121 RPS10 6204
Affinity Capture-MS Homo sapiens
122 Rps15a 267019
Co-fractionation Mus musculus
123 RSL1D1 26156
Affinity Capture-MS Homo sapiens
124 RPS24 6229
Affinity Capture-MS Homo sapiens
125 Rps21 66481
Co-fractionation Mus musculus
126 GTF3C2  
Affinity Capture-MS Homo sapiens
127 Snd1 56463
Co-fractionation Mus musculus
128 ZNF598 90850
Affinity Capture-MS Homo sapiens
129 RPL36 25873
Affinity Capture-MS Homo sapiens
130 BANF1 8815
Affinity Capture-MS Homo sapiens
131 Rplp0 11837
Co-fractionation Mus musculus
132 RPL27A 6157
Affinity Capture-MS Homo sapiens
133 RFC4 5984
Affinity Capture-MS Homo sapiens
134 DDX1 1653
Affinity Capture-MS Homo sapiens
135 DHX9 1660
Affinity Capture-MS Homo sapiens
136 RPS16 6217
Affinity Capture-MS Homo sapiens
137 PRPF31 26121
Affinity Capture-MS Homo sapiens
138 SND1 27044
Affinity Capture-MS Homo sapiens
139 RPS15A 6210
Affinity Capture-MS Homo sapiens
140 Rps16 20055
Co-fractionation Mus musculus
141 EIF3I 8668
Affinity Capture-MS Homo sapiens
142 Rps17 20068
Co-fractionation Mus musculus
143 CCNT1  
Affinity Capture-MS Homo sapiens
144 PTGR2 145482
Affinity Capture-MS Homo sapiens
145 RPL11 6135
Affinity Capture-MS Homo sapiens
146 LARP1 23367
Affinity Capture-MS Homo sapiens
147 WDR46  
Affinity Capture-MS Homo sapiens
148 TRIP13 9319
Affinity Capture-MS Homo sapiens
149 Rps18 20084
Co-fractionation Mus musculus
150 EIF3G 8666
Affinity Capture-MS Homo sapiens
151 MRM1  
Affinity Capture-MS Homo sapiens
152 CIRBP 1153
Affinity Capture-MS Homo sapiens
153 RPL32 6161
Affinity Capture-MS Homo sapiens
154 ASCC3 10973
Affinity Capture-MS Homo sapiens
155 Rpl13a 22121
Co-fractionation Mus musculus
156 SRPR 6734
Affinity Capture-MS Homo sapiens
157 CNOT7 29883
Affinity Capture-MS Homo sapiens
158 HBS1L 10767
Affinity Capture-MS Homo sapiens
159 Gnb2l1 14694
Co-fractionation Mus musculus
160 MRTO4 51154
Affinity Capture-MS Homo sapiens
161 RPS2 6187
Affinity Capture-MS Homo sapiens
162 Rpl14 67115
Co-fractionation Mus musculus
163 Rps2 16898
Co-fractionation Mus musculus
164 GTPBP4 23560
Affinity Capture-MS Homo sapiens
165 YTHDC2 64848
Affinity Capture-MS Homo sapiens
166 Rps8 20116
Co-fractionation Mus musculus
167 DHX36 170506
Affinity Capture-MS Homo sapiens
168 Rpl7a 27176
Co-fractionation Mus musculus
169 WBSCR22  
Affinity Capture-MS Homo sapiens
170 DDX50 79009
Affinity Capture-MS Homo sapiens
171 DHX29 54505
Affinity Capture-MS Homo sapiens
172 RFC2 5982
Affinity Capture-MS Homo sapiens
173 Rpl32 19951
Co-fractionation Mus musculus
174 Rps28 54127
Co-fractionation Mus musculus
175 NPM3 10360
Affinity Capture-MS Homo sapiens
176 TJP1 7082
Affinity Capture-MS Homo sapiens
177 DEK 7913
Affinity Capture-MS Homo sapiens
178 RPS8 6202
Affinity Capture-MS Homo sapiens
179 WDR55  
Affinity Capture-MS Homo sapiens
180 Tpt1 22070
Co-fractionation Mus musculus
181 ELAC2 60528
Affinity Capture-MS Homo sapiens
182 Rps11 27207
Co-fractionation Mus musculus
183 Rpl18a 76808
Co-fractionation Mus musculus
184 DHX30 22907
Affinity Capture-MS Homo sapiens
185 HDGFRP2 84717
Affinity Capture-MS Homo sapiens
186 CBX2  
Affinity Capture-MS Homo sapiens
187 RPL7 6129
Affinity Capture-MS Homo sapiens
188 LRRC8A 56262
Affinity Capture-MS Homo sapiens
189 HSD17B10 3028
Affinity Capture-MS Homo sapiens
190 GTF3C4 9329
Affinity Capture-MS Homo sapiens
191 Rpl22 19934
Co-fractionation Mus musculus
192 RFC1 5981
Affinity Capture-MS Homo sapiens
193 NTHL1  
Affinity Capture-MS Homo sapiens
194 TJP3  
Affinity Capture-MS Homo sapiens
195 EIF3H 8667
Affinity Capture-MS Homo sapiens
196 RPS27 6232
Affinity Capture-MS Homo sapiens
197 Rpl19 19921
Co-fractionation Mus musculus
198 MINA 84864
Affinity Capture-MS Homo sapiens
199 MED10  
Affinity Capture-MS Homo sapiens
200 NMT1 4836
Affinity Capture-MS Homo sapiens
201 Rpl21 19933
Co-fractionation Mus musculus
202 IFRD2 7866
Affinity Capture-MS Homo sapiens
203 PSPC1 55269
Affinity Capture-MS Homo sapiens
204 DDX47 51202
Affinity Capture-MS Homo sapiens
205 RPS13 6207
Affinity Capture-MS Homo sapiens
206 ZCCHC6 79670
Affinity Capture-MS Homo sapiens
207 REEP3 221035
Affinity Capture-MS Homo sapiens
208 MRPL46  
Affinity Capture-MS Homo sapiens
209 RPS14 6208
Affinity Capture-MS Homo sapiens
210 FOCAD 54914
Affinity Capture-MS Homo sapiens
211 Rps3a1 20091
Co-fractionation Mus musculus
212 NIPBL 25836
Affinity Capture-MS Homo sapiens
213 RPL22 6146
Affinity Capture-MS Homo sapiens
214 JUNB  
Affinity Capture-MS Homo sapiens
215 MED23 9439
Affinity Capture-MS Homo sapiens
216 RPL23A 6147
Affinity Capture-MS Homo sapiens
217 Rps14 20044
Co-fractionation Mus musculus
218 BMI1  
Affinity Capture-MS Homo sapiens
219 ZC3HAV1 56829
Affinity Capture-MS Homo sapiens
220 KIAA1522 57648
Affinity Capture-MS Homo sapiens
221 EIF2B1 1967
Affinity Capture-MS Homo sapiens
222 POGZ 23126
Affinity Capture-MS Homo sapiens
223 PACSIN3 29763
Affinity Capture-MS Homo sapiens
224 UHRF1 29128
Affinity Capture-MS Homo sapiens
225 HMG20A  
Affinity Capture-MS Homo sapiens
226 ADAR 103
Affinity Capture-MS Homo sapiens
227 Rpl5 100503670
Co-fractionation Mus musculus
228 GNB2L1 10399
Affinity Capture-MS Homo sapiens
229 Fancd2  
Affinity Capture-MS Mus musculus
230 EIF3D 8664
Affinity Capture-MS Homo sapiens
231 Rpl30 19946
Co-fractionation Mus musculus
232 Rps19 20085
Co-fractionation Mus musculus
233 Rps27a 78294
Co-fractionation Mus musculus
234 PA2G4 5036
Affinity Capture-MS Homo sapiens
235 PIP5K1A 8394
Affinity Capture-MS Homo sapiens
236 SUPT16H 11198
Affinity Capture-MS Homo sapiens
237 EIF2S1 1965
Affinity Capture-MS Homo sapiens
238 PRDM2  
Affinity Capture-MS Homo sapiens
239 SFPQ 6421
Affinity Capture-MS Homo sapiens
240 KIF2A 3796
Affinity Capture-MS Homo sapiens
241 Rps25 75617
Co-fractionation Mus musculus
242 GIGYF2 26058
Affinity Capture-MS Homo sapiens
243 RPS25 6230
Affinity Capture-MS Homo sapiens
244 ILF3 3609
Affinity Capture-MS Homo sapiens
245 Rpl15 66480
Co-fractionation Mus musculus
246 FBXL6  
Affinity Capture-MS Homo sapiens
247 AVEN 57099
Affinity Capture-MS Homo sapiens
248 ZBTB7B  
Affinity Capture-MS Homo sapiens
249 JPH1 56704
Affinity Capture-MS Homo sapiens
250 RPS21 6227
Affinity Capture-MS Homo sapiens
251 GTPBP6  
Affinity Capture-MS Homo sapiens
252 Rpl10a 19896
Co-fractionation Mus musculus
253 EIF1AX 1964
Affinity Capture-MS Homo sapiens
254 SSRP1 6749
Affinity Capture-MS Homo sapiens
255 DDX3X 1654
Affinity Capture-MS Homo sapiens
256 RPP25L  
Affinity Capture-MS Homo sapiens
257 CLP1