Gene description for Rpl13
Gene name ribosomal protein L13
Gene symbol Rpl13
Other names/aliases A52
L13
Species Mus musculus
 Database cross references - Rpl13
ExoCarta ExoCarta_270106
Vesiclepedia VP_270106
Entrez Gene 270106
UniProt P47963  
 Rpl13 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
Pancreatic cells 19351151    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl13
Molecular Function
    RNA binding GO:0003723 IBA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
Biological Process
    blastocyst development GO:0001824 IMP
    cytoplasmic translation GO:0002181 NAS
    bone development GO:0060348 IEA
    bone development GO:0060348 ISO
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
Subcellular Localization
    nucleolus GO:0005730 IEA
    nucleolus GO:0005730 ISO
    cytoplasm GO:0005737 IDA
    endoplasmic reticulum GO:0005783 IEA
    endoplasmic reticulum GO:0005783 ISO
    cytosol GO:0005829 ISO
    cytosol GO:0005829 ISS
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IDA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
 Experiment description of studies that identified Rpl13 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 188
MISEV standards
✔
EM
Biophysical techniques
✔
GAPDH|UCHL1|HSP90
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors "Lee HS, Jeong J, Lee KJ."
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
5
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl13
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Rpl28 19943
Co-fractionation Mus musculus
3 Kctd13  
Affinity Capture-MS Mus musculus
4 Elf5  
Affinity Capture-MS Mus musculus
5 Rpl31 114641
Co-fractionation Mus musculus
6 Tubgcp4 51885
Co-fractionation Mus musculus
7 Rps25 75617
Co-fractionation Mus musculus
8 Rps15a 267019
Co-fractionation Mus musculus
9 Rpl11 67025
Co-fractionation Mus musculus
10 Rpl13a 22121
Co-fractionation Mus musculus
11 Rpl15 66480
Co-fractionation Mus musculus
12 Rplp2 67186
Co-fractionation Mus musculus
13 Rpl38  
Co-fractionation Mus musculus
14 Rps4x 20102
Co-fractionation Mus musculus
15 Rpl4 67891
Co-fractionation Mus musculus
16 Rpl14 67115
Co-fractionation Mus musculus
17 Rps2 16898
Co-fractionation Mus musculus
18 Rpl10a 19896
Co-fractionation Mus musculus
19 Rps10 67097
Co-fractionation Mus musculus
20 Rps8 20116
Co-fractionation Mus musculus
21 Rps23 66475
Co-fractionation Mus musculus
22 Rpl7a 27176
Co-fractionation Mus musculus
23 Rps18 20084
Co-fractionation Mus musculus
24 Rpl27 19942
Co-fractionation Mus musculus
25 Rpl32 19951
Co-fractionation Mus musculus
26 Rpl6 19988
Co-fractionation Mus musculus
27 Fzr1  
Affinity Capture-MS Mus musculus
28 Rps19 20085
Co-fractionation Mus musculus
29 Rps26 27370
Co-fractionation Mus musculus
30 Tfe3  
Affinity Capture-MS Mus musculus
31 Rps20 67427
Co-fractionation Mus musculus
32 Rpl24 68193
Co-fractionation Mus musculus
33 Rpl35 66489
Co-fractionation Mus musculus
34 Tpt1 22070
Co-fractionation Mus musculus
35 Rps11 27207
Co-fractionation Mus musculus
36 Rpia  
Co-fractionation Mus musculus
37 Rpl23 65019
Co-fractionation Mus musculus
38 Rps5 20103
Co-fractionation Mus musculus
39 Rpl35a 57808
Co-fractionation Mus musculus
40 Rpl17 319195
Co-fractionation Mus musculus
41 Rpl22 19934
Co-fractionation Mus musculus
42 Rpl5 100503670
Co-fractionation Mus musculus
43 Tmem173  
Proximity Label-MS Mus musculus
44 Eif6 16418
Co-fractionation Mus musculus
45 Rars2  
Co-fractionation Mus musculus
46 Rps24 20088
Co-fractionation Mus musculus
47 Raver1  
Co-fractionation Mus musculus
48 Rps13 68052
Co-fractionation Mus musculus
49 Rpl7 19989
Co-fractionation Mus musculus
50 Rpl18 19899
Co-fractionation Mus musculus
51 Rpl21 19933
Co-fractionation Mus musculus
52 Rpl18a 76808
Co-fractionation Mus musculus
53 Rps21 66481
Co-fractionation Mus musculus
54 Rpl12 269261
Co-fractionation Mus musculus
55 Rpl39 67248
Co-fractionation Mus musculus
56 Rps28 54127
Co-fractionation Mus musculus
57 Gnb2l1 14694
Co-fractionation Mus musculus
58 Rps15 20054
Co-fractionation Mus musculus
59 Eef1a1 13627
Co-fractionation Mus musculus
60 Rps3a1 20091
Co-fractionation Mus musculus
61 Pde12  
Co-fractionation Mus musculus
62 Rpl36a 19982
Co-fractionation Mus musculus
63 Rps9 76846
Co-fractionation Mus musculus
64 Rpl26 19941
Co-fractionation Mus musculus
65 Rpl13 270106
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
66 Rplp0 11837
Co-fractionation Mus musculus
67 Rpl23a 268449
Co-fractionation Mus musculus
68 Rps14 20044
Co-fractionation Mus musculus
69 Rps17 20068
Co-fractionation Mus musculus
70 Rpl30 19946
Co-fractionation Mus musculus
71 Eed  
Affinity Capture-MS Mus musculus
72 Rpsa 16785
Co-fractionation Mus musculus
73 Eef1b2 55949
Co-fractionation Mus musculus
74 Rpl3 27367
Co-fractionation Mus musculus
75 Tia1  
Affinity Capture-MS Mus musculus
76 Rps16 20055
Co-fractionation Mus musculus
77 Fancd2  
Affinity Capture-MS Mus musculus
78 Rpl8 26961
Co-fractionation Mus musculus
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