ExoCarta: Gene summary

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Gene description for Rps25
Gene name	ribosomal protein S25
Gene symbol	Rps25
Other names/aliases	2810009D21Rik
Species	Mus musculus
Database cross references - Rps25
ExoCarta	ExoCarta_75617
Entrez Gene	75617
UniProt	P62852
Rps25 identified in exosomes derived from the following tissue/cell type
Mast cells	17486113

Gene ontology annotations for Rps25

Molecular Function
	poly(A) RNA binding	GO:0044822	ISO
	structural constituent of ribosome	GO:0003735	ISO
Biological Process
	ribosomal small subunit assembly	GO:0000028	ISO
Subcellular Localization
	small ribosomal subunit	GO:0015935	ISO
	extracellular exosome	GO:0070062	ISO
	cytosolic small ribosomal subunit	GO:0022627	ISO
	ribonucleoprotein complex	GO:0030529	IEA
	nucleus	GO:0005634	ISO
	nucleolus	GO:0005730	ISO
	cytoplasm	GO:0005737	ISO
	ribosome	GO:0005840	ISO

Experiment description of studies that identified Rps25 in exosomes

Experiment ID

ISEV standards
✔ EM	EV Biophysical techniques
✘	EV Cytosolic markers
✔ CD63	EV Membrane markers
✘	EV Negative markers
✘	EV Particle analysis

Identified molecule

protein

Identification method

Mass spectrometry

PubMed ID

17486113

Organism

Mus musculus
Homo sapiens

Experiment description

Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.

Authors

Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO

Journal name

NCB

Publication year

2007

Sample

Mast cells

Sample name

MC9
Bone marrow-derived mast cells
HMC-1

Isolation/purification methods

Filtration
Ultracentrifugation
Sucrose density gradient

Flotation density

1.11-1.21 g/mL

Molecules identified in the study

Protein
mRNA
miRNA

Methods used in the study

Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array