Gene description for Rpl12
Gene name ribosomal protein L12
Gene symbol Rpl12
Other names/aliases E430018F03
Species Mus musculus
 Database cross references - Rpl12
ExoCarta ExoCarta_269261
Vesiclepedia VP_269261
Entrez Gene 269261
UniProt P35979  
 Rpl12 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rpl12
Molecular Function
    RNA binding GO:0003723 IEA
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IEA
    structural constituent of ribosome GO:0003735 ISO
    protein binding GO:0005515 IPI
    large ribosomal subunit rRNA binding GO:0070180 IBA
    large ribosomal subunit rRNA binding GO:0070180 ISO
Biological Process
    cytoplasmic translation GO:0002181 NAS
    translation GO:0006412 IBA
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
Subcellular Localization
    nucleus GO:0005634 IDA
    nucleolus GO:0005730 IDA
    cytoplasm GO:0005737 IDA
    cytosol GO:0005829 TAS
    ribosome GO:0005840 NAS
    postsynaptic density GO:0014069 IEA
    postsynaptic density GO:0014069 ISO
    cytosolic large ribosomal subunit GO:0022625 IBA
    cytosolic large ribosomal subunit GO:0022625 IPI
    cytosolic large ribosomal subunit GO:0022625 ISO
    cytosolic ribosome GO:0022626 ISO
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
 Experiment description of studies that identified Rpl12 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rpl12
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Rps3 27050
Co-fractionation Mus musculus
3 Rpl11 67025
Co-fractionation Mus musculus
4 Kctd13  
Affinity Capture-MS Mus musculus
5 Fxr1 14359
Affinity Capture-MS Mus musculus
6 Rps18 20084
Co-fractionation Mus musculus
7 Rps25 75617
Co-fractionation Mus musculus
8 Rpl28 19943
Co-fractionation Mus musculus
9 Rpl13a 22121
Co-fractionation Mus musculus
10 Rpl15 66480
Co-fractionation Mus musculus
11 Rplp2 67186
Co-fractionation Mus musculus
12 Rpl38  
Co-fractionation Mus musculus
13 Rps4x 20102
Co-fractionation Mus musculus
14 Rpl14 67115
Co-fractionation Mus musculus
15 Rps2 16898
Co-fractionation Mus musculus
16 Kcnma1  
Affinity Capture-MS Mus musculus
17 Rps23 66475
Co-fractionation Mus musculus
18 Rpl7a 27176
Co-fractionation Mus musculus
19 Rpl27 19942
Co-fractionation Mus musculus
20 Rpl3 27367
Co-fractionation Mus musculus
21 Rpl32 19951
Co-fractionation Mus musculus
22 Rpl6 19988
Co-fractionation Mus musculus
23 Fzr1  
Affinity Capture-MS Mus musculus
24 Rps19 20085
Co-fractionation Mus musculus
25 Rps26 27370
Co-fractionation Mus musculus
26 Tfe3  
Affinity Capture-MS Mus musculus
27 Rpl9 20005
Co-fractionation Mus musculus
28 Ola1 67059
Co-fractionation Mus musculus
29 Rps24 20088
Co-fractionation Mus musculus
30 Rpl18a 76808
Co-fractionation Mus musculus
31 Rpl23 65019
Co-fractionation Mus musculus
32 Rps5 20103
Co-fractionation Mus musculus
33 Rps10 67097
Co-fractionation Mus musculus
34 Rpl17 319195
Co-fractionation Mus musculus
35 Rpl22 19934
Co-fractionation Mus musculus
36 Rpl5 100503670
Co-fractionation Mus musculus
37 Eif6 16418
Co-fractionation Mus musculus
38 Rps20 67427
Co-fractionation Mus musculus
39 Pa2g4 18813
Co-fractionation Mus musculus
40 Rpl7 19989
Co-fractionation Mus musculus
41 Rpl18 19899
Co-fractionation Mus musculus
42 Rps9 76846
Co-fractionation Mus musculus
43 Rps13 68052
Co-fractionation Mus musculus
44 Rps21 66481
Co-fractionation Mus musculus
45 Rpl12 269261
Cross-Linking-MS (XL-MS) Mus musculus
Cross-Linking-MS (XL-MS) Mus musculus
46 Rps15a 267019
Co-fractionation Mus musculus
47 Gnb2l1 14694
Co-fractionation Mus musculus
48 Rpl35 66489
Co-fractionation Mus musculus
49 Rps7 20115
Co-fractionation Mus musculus
50 Rps3a1 20091
Co-fractionation Mus musculus
51 Rpl36a 19982
Co-fractionation Mus musculus
52 Rpl26 19941
Co-fractionation Mus musculus
53 Rpl13 270106
Co-fractionation Mus musculus
54 Rplp0 11837
Co-fractionation Mus musculus
55 Rpl23a 268449
Co-fractionation Mus musculus
56 Rps14 20044
Co-fractionation Mus musculus
57 Rpl30 19946
Co-fractionation Mus musculus
58 Eed  
Affinity Capture-MS Mus musculus
59 Rpsa 16785
Co-fractionation Mus musculus
60 Eef1b2 55949
Co-fractionation Mus musculus
61 Mapt  
Affinity Capture-MS Mus musculus
62 Canx 12330
Co-fractionation Mus musculus
63 Skiv2l2  
Co-fractionation Mus musculus
64 Nedd8  
Co-fractionation Mus musculus
65 Rpl4 67891
Co-fractionation Mus musculus
66 Fancd2  
Affinity Capture-MS Mus musculus
67 Rpl8 26961
Co-fractionation Mus musculus
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