Gene description for Rps26
Gene name ribosomal protein S26
Gene symbol Rps26
Other names/aliases -
Species Mus musculus
 Database cross references - Rps26
ExoCarta ExoCarta_27370
Vesiclepedia VP_27370
Entrez Gene 27370
UniProt P62855  
 Rps26 identified in sEVs derived from the following tissue/cell type
Colon cancer cells 37309723    
Mast cells 17486113    
T-cell lymphoma cells 37309723    
 Gene ontology annotations for Rps26
Molecular Function
    mRNA binding GO:0003729 IBA
    mRNA binding GO:0003729 IEA
    mRNA binding GO:0003729 ISO
    structural constituent of ribosome GO:0003735 IBA
    structural constituent of ribosome GO:0003735 IDA
    structural constituent of ribosome GO:0003735 ISO
Biological Process
    cytoplasmic translation GO:0002181 ISO
    cytoplasmic translation GO:0002181 ISS
    cytoplasmic translation GO:0002181 NAS
    negative regulation of RNA splicing GO:0033119 IEA
    negative regulation of RNA splicing GO:0033119 ISO
    translation at presynapse GO:0140236 NAS
    translation at postsynapse GO:0140242 NAS
Subcellular Localization
    cytoplasm GO:0005737 NAS
    cytosol GO:0005829 TAS
    ribosome GO:0005840 ISO
    ribosome GO:0005840 ISS
    ribosome GO:0005840 NAS
    cytosolic small ribosomal subunit GO:0022627 IBA
    cytosolic small ribosomal subunit GO:0022627 IDA
    cytosolic small ribosomal subunit GO:0022627 ISO
    cytosolic small ribosomal subunit GO:0022627 ISS
    cytosolic small ribosomal subunit GO:0022627 NAS
    synapse GO:0045202 EXP
    synapse GO:0045202 IDA
    cytoplasmic side of rough endoplasmic reticulum membrane GO:0098556 ISS
    presynapse GO:0098793 NAS
    postsynapse GO:0098794 NAS
 Experiment description of studies that identified Rps26 in sEVs
1
Experiment ID 907
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample Colon cancer cells
Sample name C26
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
2
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
MISEV standards
✔
EM
Biophysical techniques
✔
CD63
Enriched markers
✘ Negative markers
✘ Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors "Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO"
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 908
MISEV standards
✔
EM
Biophysical techniques
✔
Tsg101|Sdcbp|Cd151|Gapdh|Lamp2|Cd81|Cd82|Cd9|Cd63|Cd80|Flot2|Tfrc|Rab35|Rab5a
Enriched markers
✔
HSPA5
Negative markers
✔
NTA
Particle analysis
Identified molecule Protein
Identification method Mass spectrometry
PubMed ID 37309723    
Organism Mus musculus
Experiment description Proteomic analysis of the small extracellular vesicles and soluble secretory proteins from cachexia inducing and non-inducing cancer cells
Authors "Chitti SV, Kang T, Fonseka P, Marzan AL, Stewart S, Shahi S, Bramich K, Ang CS, Pathan M, Gummadi S, Mathivanan S."
Journal name Proteomics
Publication year 2023
Sample T-cell lymphoma cells
Sample name EL4
Isolation/purification methods Differential centrifugation
Ultracentrifugation
Flotation density -
Molecules identified in the study Protein
Methods used in the study Western blotting
Mass spectrometry
 Protein-protein interactions for Rps26
  Protein Interactor ExoCarta ID Identification method PubMed Species
1 Rps27a 78294
Co-fractionation Mus musculus
2 Rps18 20084
Co-fractionation Mus musculus
3 Eif3a 13669
Co-fractionation Mus musculus
4 Rps25 75617
Co-fractionation Mus musculus
5 Rpl15 66480
Co-fractionation Mus musculus
6 Rplp2 67186
Co-fractionation Mus musculus
7 Rpl38  
Co-fractionation Mus musculus
8 Rps4x 20102
Co-fractionation Mus musculus
9 Rpl14 67115
Co-fractionation Mus musculus
10 Rpl32 19951
Co-fractionation Mus musculus
11 Trp53  
Affinity Capture-Western Mus musculus
12 Mapt  
Affinity Capture-MS Mus musculus
13 Dync1i2  
Co-fractionation Mus musculus
14 Kctd13  
Affinity Capture-MS Mus musculus
15 Rpl24 68193
Co-fractionation Mus musculus
16 Rps24 20088
Co-fractionation Mus musculus
17 Rpl18a 76808
Co-fractionation Mus musculus
18 Serbp1 66870
Co-fractionation Mus musculus
19 Rps7 20115
Co-fractionation Mus musculus
20 Rpl22 19934
Co-fractionation Mus musculus
21 Rps6 20104
Co-fractionation Mus musculus
22 Rpl12 269261
Co-fractionation Mus musculus
23 Rps21 66481
Co-fractionation Mus musculus
24 Rps15a 267019
Co-fractionation Mus musculus
25 Gnb2l1 14694
Co-fractionation Mus musculus
26 Rps13 68052
Co-fractionation Mus musculus
27 Fancd2  
Affinity Capture-MS Mus musculus
28 Rps14 20044
Co-fractionation Mus musculus
29 Rpl30 19946
Co-fractionation Mus musculus
30 Eed  
Affinity Capture-MS Mus musculus
31 Tsr2  
Co-fractionation Mus musculus
32 Rps27 57294
Co-fractionation Mus musculus
33 Rpsa 16785
Co-fractionation Mus musculus
34 Eef1b2 55949
Co-fractionation Mus musculus
35 Tpi1 21991
Co-fractionation Mus musculus
36 Rps16 20055
Co-fractionation Mus musculus
37 Otx2  
Affinity Capture-MS Mus musculus
38 Rpl13 270106
Co-fractionation Mus musculus
39 Rpl8 26961
Co-fractionation Mus musculus
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