Gene description for Rpsa
Gene name ribosomal protein SA
Gene symbol Rpsa
Other names/aliases 67kDa
67lr
AL022858
Lamr
Lamr1
Lamrl1
MLR
P40
P40-3
P40-8
Species Mus musculus
 Database cross references - Rpsa
ExoCarta ExoCarta_16785
Entrez Gene 16785
UniProt P14206  
 Rpsa identified in exosomes derived from the following tissue/cell type
Brain cancer cells 19109410    
Mast cells 17486113    
Pancreatic cells 19351151    
 Gene ontology annotations for Rpsa
Molecular Function
    poly(A) RNA binding GO:0044822 ISO
    ribosome binding GO:0043022 ISO
    laminin binding GO:0043236 IDA
    structural constituent of ribosome GO:0003735 IBA
    laminin receptor activity GO:0005055 IEA
Biological Process
    ribosomal small subunit assembly GO:0000028 IBA
    endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000461 IBA
    single organismal cell-cell adhesion GO:0016337 ISO
    endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) GO:0000447 IBA
    translation GO:0006412 IBA
    rRNA export from nucleus GO:0006407 IBA
Subcellular Localization
    extracellular matrix GO:0031012 ISO
    90S preribosome GO:0030686 IBA
    intracellular GO:0005622 IEA
    nucleus GO:0005634 IDA
    extracellular exosome GO:0070062 ISO
    cytoplasm GO:0005737 ISO
    small ribosomal subunit GO:0015935 IDA
    plasma membrane GO:0005886 ISO
    cytosolic small ribosomal subunit GO:0022627 ISO
    basement membrane GO:0005604 ISO
    ribonucleoprotein complex GO:0030529 IEA
    cytosol GO:0005829 ISO
    neuronal cell body GO:0043025 ISO
    membrane GO:0016020 ISO
    ribosome GO:0005840 IEA
 Experiment description of studies that identified Rpsa in exosomes
1
Experiment ID 26
ISEV standards
EM
EV Biophysical techniques
Alix|GAPDH|HSP70
EV Cytosolic markers
CD9
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19109410    
Organism Mus musculus
Experiment description Proteomic and immunologic analyses of brain tumor exosomes.
Authors Graner MW, Alzate O, Dechkovskaia AM, Keene JD, Sampson JH, Mitchell DA, Bigner DD
Journal name FASEB
Publication year 2008
Sample Brain cancer cells
Sample name SMA560vIII
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density 1.13-1.15 g/mL
Molecules identified in the study Protein
Methods used in the study Mass spectrometry [MALDI TOF/TOF]
Western blotting
2
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
3
Experiment ID 15
ISEV standards
EM
EV Biophysical techniques
EV Cytosolic markers
CD63
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule mrna
Identification method Microarray
PubMed ID 17486113    
Organism Mus musculus
Homo sapiens
Experiment description Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells.
Authors Valadi H, Ekstrom K, Bossios A, Sjostrand M, Lee JJ, Lotvall JO
Journal name NCB
Publication year 2007
Sample Mast cells
Sample name MC9
Bone marrow-derived mast cells
HMC-1
Isolation/purification methods Filtration
Ultracentrifugation
Sucrose density gradient
Flotation density 1.11-1.21 g/mL
Molecules identified in the study Protein
mRNA
miRNA
Methods used in the study Mass spectrometry [MALDI TOF]
Western blotting
Microarray
miRCURY LNA Array
4
Experiment ID 188
ISEV standards
EM
EV Biophysical techniques
GAPDH|UCHL1|HSP90
EV Cytosolic markers
EV Membrane markers
EV Negative markers
EV Particle analysis
Identified molecule protein
Identification method Mass spectrometry
PubMed ID 19351151    
Organism Mus musculus
Experiment description Characterization of vesicles secreted from insulinoma NIT-1 cells.
Authors Lee HS, Jeong J, Lee KJ.
Journal name J Proteome Res
Publication year 2009
Sample Pancreatic cells
Sample name Pancreatic beta cell (NIT-1)
Isolation/purification methods Differential centrifugation
Sucrose density gradient
Flotation density -
Molecules identified in the study Protein
Methods used in the study Mass spectrometry
 Protein-protein interactions for Rpsa
  Protein Interactor ExoCarta ID Identification method PubMed Species
No interactions are found.



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